High-quality 25-Acetoxy Bivittoside Production with Advanced Technology
At CD BioGlyco, our scientists pay extra attention to Marine Biomolecule Production. From Marine Carbohydrates to Marine Glycosides and Glycolipids, our specialized technical team is constantly expanding the range of marine biomolecules produced. Among marine organisms, we mainly offer the extraction of saponins from sea cucumbers, sea stars, and sponges. Specifically, marine-derived saponins differ significantly from terrestrial saponins.
Here, we not only provide 25-acetoxy bivittoside production service but also include a series of services such as structure identification, molecular weight test, and activity analysis.
Sample Preparation
Our researchers dry the collected marine samples and preserve them. All you need to do is to give us the samples. We have extensive experience in sample pre-processing. For example, from sea cucumbers, we offer saponin extraction services from different parts of the body including, but not limited to, the body wall, digestive glands, and Juvenile ducts.
Extraction and Purification
CD BioGlyco offers a wide range of high-quality extraction services including reflux extraction, enzymatic extraction, semi-bionic extraction, microwave-assisted extraction, organic solvent extraction, and ultrasonic extraction. Depending on the nature of the raw material, our lab provides the most satisfactory extraction solution to our clients.
CD BioGlyco provides high-purity 25-acetoxy bivittoside purification services by high-performance liquid chromatography (HPLC) purification, macroporous adsorbent resin purification, silica gel column chromatography purification, and dextran gel column purification. It is difficult to completely separate the target compounds by a single separation technique. In our lab, the target compounds are enriched by macroporous adsorbent resin, and then coarsely separated by normal-phase silica gel, dextran gel, reversed-phase silica gel, etc., and then purified by preparative HPLC.
Analysis
We use nuclear magnetic resonance (NMR), Fourier-transform-infrared-spectroscopy (FTIR), electrospray ionization-tandem mass spectrometry (ESI-MS/MS), and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) to analyze the structure of the target.
Publication
Technology: High-performance centrifugal partition chromatography (HPCPC), MALDI-MS/MS, ESI-MS/MS
Journal: Marine Drugs
Published: 2018
IF: 5.4
Results: In this work, the researchers used ethanol extraction and HPCPC to isolate high-purity saponins originating from the sea cucumber body wall. The researchers used MALDI-MS/MS and ESI-MS/MS to analyze and characterize the saponins. The results showed that 89 saponin homologs were present in the sea cucumber body wall, including 35 new saponins and 54 known saponins. These include 25-acetoxy bivittoside D, 17α-hydroxy impatienside A, marmoratoside B and so on. In addition, the authors discuss the antifungal activity of four novel acetylated saponins.
Fig.1 ESI-MS detection pattern of Fraction 110 in different ionic modes. (Bahrami, et al., 2018)
Applications
- 25-Acetoxy bivittoside has a wide range of biological activities that can be used for the development of new marine drugs.
- 25-Acetoxy bivittoside has a variety of structurally similar glycosides and can be used for glycoside conformational relationship studies.
- 25-Acetoxy bivittoside provides valuable information for the development of marine health foods.
Advantages of Us
- We not only provide isolation and identification of target molecules but also provide professional analysis and characterization services for components of interest to our customers.
- Combined with multiple separation techniques, we ensure high-purity 25-acetoxy bivittoside for our clients.
- We have a wide range of cell and mouse models to meet our clients' needs for glycoside bioactivity testing.
Frequently Asked Questions
What are the biological activities of sea cucumber saponins?
Sea cucumber saponins have antidiabetic, antioxidant, anti-asthmatic, anti-eczema, anti-arthritic, cholesterol-lowering effects, immunomodulatory, cytotoxic, anti-helminthic, anti-viral, anti-fungal, anti-angiogenic, anti-proliferative, and anti-dementia activities.
How do macroporous adsorbent resin separate saponins?
Macroporous adsorbent resin is a kind of high molecular adsorbent resin without exchange groups and with a large pore structure, which selectively adsorbs target components from aqueous and non-aqueous systems through hydrogen bonding interaction, van der Waals force (hydrophobic interaction), complexation, sieving, and electrostatic force. Separation can be achieved based on differences in the affinity of the adsorbent for the adsorbate molecules.
CD BioGlyco is committed to providing our clients with the most reliable 25-acetoxy bivittoside production solutions possible. Depending on the client's needs, we provide small, medium, and large batch manufacturing services. Our technicians have extensive experience in the production and characterization of marine glycobiology. Please feel free to contact us for more information.
Reference
- Bahrami, Y.; et al. Distribution of saponins in the sea cucumber Holothuria lessoni; the body wall versus the viscera, and their biological activities. Marine Drugs. 2018, 16(11): 423.
