CD BioGlyco's Premier Stichoposide Production: High-Quality Marine Glycosides for Cancer Research
CD BioGlyco provides the services to produce stichoposide D and C, two triterpene glycosides found in a range of sea cucumbers like Stichopus chloronotus, Thelenota ananas, and Thelenota anax. These compounds process remarkable antitumor activity, especially those that can induce apoptosis in leukemia and colorectal cancer cell lines. Our process is to extract it from sea cucumbers, purify the glycosides, and then characterize them in some way that their bioactivity can be demonstrated. CD BioGlyco offers Marine Glycoside Products, engineered using advanced and superior methods which are perfect for research or therapeutic development purposes.
Fig.1 The structures of stichoposide C and stichoposide D. (Yun, et al., 2016)
Stichoposides C and D are valuable for developing new cancer treatments and furthering research into marine-derived anticancer agents. Here are CD BioGlyco's advanced and outstanding details of stichoposide production service. Please feel free to browse our Marine Biomolecule Production Service if you are interested in our other marine biomolecule products!
Collection and Initial Extraction
Sea cucumbers are collected from their natural environments, and their tissues are processed by immersing them in methanol (MeOH) at ambient temperature. This first extraction phase is crucial for extracting the crude mixture of bioactive compounds, particularly glycosides, from the tissues.
Partitioning
The resulting crude extract from the initial extraction is then divided into two phases using a mixture of 90% aqueous methanol (MeOH(aq)) and hexane. This partitioning process is vital for distinguishing between polar and nonpolar substances, thereby concentrating the glycosides in the methanol phase.
Fractionation
The methanol fraction was subsequently fractionated by flash chromatography, on a column of ODS-A. This method separates the mixture into a wide array of fractions based on their chemical properties.
Quality Testing
The combined active fractions are further purified using semi-preparative reverse-phase high-performance liquid chromatography (RP-HPLC). This stage of the process utilizes a Zorbax ODS column, where a gradient of acetonitrile (CH3CN) in water (H2O) with trifluoroacetic acid (TFA) is applied as the mobile phase. This specific setup is critical for effectively separating the components. The stichoposides C and D are isolated during this process by carefully monitoring their unique retention times and interactions with the stationary phase of the column, ensuring high purity and specificity of the glycosides.
Verification
To confirm the purity and molecular weight of the isolated stichoposides, high-resolution electrospray ionization mass spectrometry (HRESIMS) is utilized. This technique provides precise measurements of the molecular weight, ensuring the compounds are correctly identified. Additionally, nuclear magnetic resonance (NMR) spectroscopy, encompassing both 1H and 13C NMR, is conducted to thoroughly verify the chemical structure of the stichoposides. These NMR analyses are crucial for confirming the arrangement of atoms within the molecules, thus guaranteeing the structural integrity and authenticity of the glycosides.
Publication
DOI: https://doi.org/10.3390/md14110205
Technology: RP-HPLC, HRESIMS, NMR
Journal: Marine Drugs
IF: 4.9
Published: 2016
Results: The study demonstrates that stichoposides C and D, triterpene glycosides derived from sea cucumbers, show significant antitumor activity by inducing apoptosis in leukemia and colorectal cancer cells. The research employed various techniques including methanol extraction, partitioning with hexane, flash chromatography, and RP-HPLC for purification. HRESIMS and NMR spectroscopy were used for structural verification. The findings suggest that these glycosides work by activating ceramide pathways, making them promising candidates for cancer treatment.
Applications
- Stichoposides are primarily used in pharmaceutical development for creating new cancer treatments. They are also valuable in biomedical research to study mechanisms of apoptosis and ceramide pathways in cancer cells.
- Stichoposides can be used in various biomedical research applications to explore their effects on cell death pathways, including apoptosis, and their potential uses in treating other diseases where these pathways are involved.
Advantages
- CD BioGlyco uses precise extraction and purification techniques to maintain the bioactive properties of stichoposides, ensuring their effectiveness in research and therapeutic applications.
- Regular quality checks and advanced verification methods are employed throughout CD BioGlyco's production process.
At CD BioGlyco, our advanced production and purification services ensure that researchers and pharmaceutical developers receive the highest quality stichoposides for their scientific and therapeutic development needs. Please do not hesitate to contact us for more information if you are interested in our services and technologies.
Frequently Asked Questions
What is Stichoposide and why is it important?
Stichoposides C and D are triterpene glycosides derived from sea cucumbers, known for their significant antitumor activities, particularly in inducing apoptosis in leukemia and colorectal cancer cells. These compounds hold promise for developing new cancer treatments.
How does CD BioGlyco ensure the purity of stichoposides?
We employ advanced purification techniques, including semi-preparative RP-HPLC. The purity and molecular structure of the stichoposides are verified using HRESIMS and NMR spectroscopy.
How can I start using CD BioGlyco's stichoposide production service?
To get started, contact CD BioGlyco through our website or client service. We will provide guidance on the process, discuss your specific needs, and offer a custom solution for stichoposide production.
References
- Yun, S.; et al. Ceramide as a target of marine triterpene glycosides for treatment of human myeloid leukemia. Marine Drugs. 2016, 14(11): 205.
- Safi, C.; et al. Neochloris oleoabundans from nature to industry: a comprehensive review. Reviews in Environmental Science and Bio/Technology. 2021.
- From Wikimedia: https://upload.wikimedia.org/wikipedia/commons/8/86/Thelenota_ananas.jpg.
