Driving Sokodoside Production with Confidence
Marine Glycosides are a specialized class of Marine Biomolecule found in marine organisms. They have diverse structures and biological activities and are commonly associated with the growth, immune system, and environmental adaptations of marine organisms. At CD BioGlyco, we are committed to providing our clients with structure-specific sokodoside production services such as sokodoside A and sokodoside B.
Biological Material
Depending on the client's needs, Our lab provides professional biological material collection services. We provide raw material cleaning, cutting and homogenizing services.
Isolation of Sokodosides
For homogenized biological samples, we use n-PrOH and water for extraction. After mixing the extract with water and trichloromethane, our researchers quickly isolate the target mixture. It is worth mentioning that we have specialized personnel to provide antifungal fraction isolation services. The antifungal fractions are separated by n-PrOH, water, and trifluoroacetic acid (TFA), and then the antifungal fractions are separated using a phenylhexyl column.
Purification of Sokodosides
CD BioGlyco provides efficient purification services by octadecylsilyl flash chromatography and high-performance liquid chromatography (HPLC). Our lab operators strictly control every step of the separation in order to give our clients the best sokodoside delivery.
Characterization of Sokodosides
Our trained analytical team provides conformational analysis of glycosides, monosaccharide composition, derivatization labeling, and gas chromatography-mass spectrometry (GC-MS) analysis. Moreover, we use nuclear magnetic resonance (NMR) and tandem mass spectrometry to characterize the sokodoside structure. Importantly, we offer bioactivity assay services to ensure the integrity of our services.
Publication
Journal: Marine Drugs
Published: 2010
IF: 4.9
Results: In this study, scholars discuss sokodoside A and sokodoside B derived from marine sponge E. placenta. These two sokodosides showed antifungal activity but no bacteriostatic effect against Mortierella ramanniana and Saccharomyces cereivisiae. In addition, sokodoside A and sokodoside B showed cytotoxic activity against P388 cells.
Fig.1 The structure of sokodoside A and B. (Ebada, et al., 2010)
Applications
- Sokodoside has a variety of biological activities such as antioxidant, anti-inflammatory, anti-tumor, etc. Therefore, it can be used for the research and development of new marine drugs.
- Production of structurally defined sokodosides facilitates in-depth exploration of structure-activity relationships of novel steroidal glycosides.
- Sokodoside may have a protective effect against neurodegenerative diseases therefore it can be used in the study of protective mechanisms in neurodegenerative diseases.
Advantages of Us
- CD BioGlyco has experts to help clients solve problems in marine glycoside production.
- Our researchers have many years of experience in extracting and isolating sokodosides quickly under mild conditions.
- Our company supports clients to provide their raw materials for extraction.
- With strict quality control system, we provide HPLC, GC-MS, NMR and other product data of sokodosides to ensure the purity of our products.
Frequently Asked Questions
What are the structural peculiarities of sokodoside A and B?
Sokodoside A is a branched tetraoside composed of β-D-galacturonic acid, α-L-fucose, β-D-galacturonic acid, and α-L-arabinose. Sokodoside B is a branched trioside with a carbohydrate chain composed of α-L arabinose, β-D-galacturonic acid, and β-D-galactogalacturonic acid.
What are the advantages of the hydro-alcoholic extraction method?
In the water-alcohol extraction method, the solution interacts with the glycoside molecules by forming hydrogen bonds or van der Waals forces, causing the glycosides to dissolve in the solvent. In addition, water and alcohol have some selective extraction effect on glycosides, which can better extract the target substances from the mixture. The use of the water-alcohol extraction method can efficiently extract glycosides and does not cause damage to the glycoside molecules during the extraction process, ensuring the biological activity of glycosides.
Relying on advanced production technology, CD BioGlyco provides high-yield and high-purity sokodoside production services to meet clients' needs in different fields. Please do not hesitate to contact us for more details if you are interested in our glycoside production and characterization.
Reference
- Ebada, S.S.; et al. Bioactive sesterterpenes and triterpenes from marine sponges: Occurrence and pharmacological significance. Marine Drugs. 2010, 8(2): 313-346.
