Heparan Sulfate-based Drug-Delivery Vehicle Development Service

Heparan sulfate (HS) contains a linear chain of alternating iduronic acid or D-glucuronic acid along with acetylated or sulfated D-glucosamine residues. HS is a Marine Carbohydrate with low toxicity, good biodegradability, and high biocompatibility. Moreover, HS is not easily attacked by macrophages or the complement system and thus has long-term circulatory effects. Based on these properties, CD BioGlyco offers a range of services based on Drug-Delivery Vehicle for rapid drug release and improved efficacy.

HS-based Drug-Delivery Vehicle Development Service at CD BioGlyco

• Preparation of HS
  The preparation of high-purity HS is the basis for the development of drug-delivery vehicles. At CD BioGlyco, we offer HS purification services and synthetic services. For the purified HS, we provide some characterization services including agarose-gel electrophoretic analysis, molecular weight analysis, and qualitative and quantitative analysis services.
• Synthesis and Characterization of Drug-delivery Vehicle
  Our researchers have rich experience in drug-delivery vehicle design and synthesis and quickly and accurately synthesize drug drug-delivery vehicles through a two-step amidation reaction. Meanwhile, the drug-loaded micelle is synthesized using the ultrasonic method. We provide the following analysis and detection services:

• In Vitro Assay Service
  We examine micelles' in vitro reduction-triggered release by simulating the in vivo environment. In addition, we provide In vitro cytotoxicity assays and cellular uptake mechanism study services. Our lab has professional data analysts and comprehensive instruments to meet the professional analysis needs.

Fig.1 Schematic illustration of HS-based drug-delivery vehicle synthesis and release. (CD BioGlyco)

Publication

Technology: Dynamic light scattering, Flow cytometry, and confocal laser scanning microscopy (CLSM)

Journal: Materials Science & Engineering C-Materials for Biological Applications

Published: 2019

IF: 7.9

Results: In this study, modern separation techniques combined with chromatography were utilized to prepare high-purity HS in large quantities. Furthermore, develop reductively sensitive amphiphilic polymers for doxorubicin (DOX) delivery. The results showed that the heparan sulfate-s-s-vitamin E succinate (HSV) polymers self-assembled in aqueous solution to form micelles. The DOX/HSV micelles remained stable in plasma. In vitro triggered drug release and cellular experiments showed that reduction-sensitive amphiphilic polymers transported DOX to the nucleus more efficiently than reduction-insensitive micelles. Flow cytometry (FCM) and confocal laser scanning microscopy (CLSM) further validated the intracellular drug release effect and the internalization pathway of DOX-loaded micelles. In conclusion, the reductively sensitized amphiphilic DOX/HSV micelles are effective drug carriers to deliver antitumor drugs to lesion sites.

Fig.2 Factor design results. (Qiu, et al., 2019)

Applications

• The HS-based drug-delivery vehicle is an effective vehicle to accurately aid the drug DOX into tumor tissue and successfully release.
• HS-based drug-delivery vehicles play a key role in anticancer therapy to maximize therapeutic efficacy and reduce side effects.

Advantages of Us

• We use modern separation techniques combined with chromatography to prepare highly purity HS to avoid the delivery vehicle synthesis affected by impurities.
• According to client needs, we also provide HS-based delivery vehicles with redox characteristics.
• We develop the best experimental plan for you based on your specific needs to help you develop products with market advantages.
• We have a complete characterization and in vitro assay process and continue to optimize the vector based on test results.

CD BioGlyco has a trained operating team and extensive experience in Marine Biomolecule Production, Characterization, and Modification to provide satisfactory service to our clients. We have always been well-received by clients with high-level service and reliable data. To learn more information about HS-based drug-delivery vehicles, please feel free to contact us.