Unlocking the Power of Marine Microbial Through Polysaccharide Production
Polysaccharides of marine microbial origin have novel structures and rich and diverse biological activities and are a class of Marine Carbohydrates with a wide range of uses and development potential. At CD BioGlyco, we have been conducting research on the production of Marine Biomolecules for a long time and have accumulated a lot of experience. Here, we provide marine microbial polysaccharide production services to clients all over the world with optimized Technologies for Carbohydrate Production and Purification.
Extraction of marine microbial polysaccharide
Microbial polysaccharides are water-soluble macromolecules that are usually extracted by precipitation. The microorganisms are crushed, and centrifuged, and the supernatant is added to an organic solvent, a salt solution, or an acid solution so that the polysaccharides form a precipitate and are separated from the impurities.
Alcohol precipitation is a commonly used method. After the precipitation of polysaccharides, we need to add organic solutions and complex enzymes to remove the lipids and proteins, to obtain the crude polysaccharides.
In addition, some darker-colored crude polysaccharides need to be decolorized. We provide the hydrogen oxide method, activated carbon adsorption method, etc. for decolorization.
Isolation and purification of marine microbial polysaccharide
Polysaccharides from different sources have different characteristics, so we use different purification methods according to the characteristics of polysaccharides.
- Graded alcohol precipitation method
Since polysaccharides with different molecular weights have different solubility in ethanol, we use this method to isolate polysaccharides with a single molecular weight.
- Chromatography
Chromatography is a commonly used method for the separation and purification of polysaccharides and uses different chromatographic techniques to purify polysaccharides from some perspectives.
- Volume exclusion chromatography: This method allows the separation of polysaccharides in a specific molecular weight range.
- Ion exchange chromatography: This method allows the separation and purification of polysaccharides with different charges and with different polarities.
- Affinity chromatography: This method separates polysaccharides from other biomolecules to obtain polysaccharide purities.
- Membrane separation
Membrane separation methods include dialysis, ultrafiltration, and electrodialysis, which are suitable for the separation and purification of polysaccharides of different molecular weights.
Characterization of marine microbial polysaccharide
- Structural analysis
- Nuclear magnetic resonance spectroscopy (NMR): The 1H NMR and 13C NMR can be used to analyze the straight-chain or cyclic structure of polysaccharides, the configuration of glycosidic bonds, and the ratio of the composition of different glycosidic bonds.
- Infrared spectroscopy (IR): The characteristic absorption peaks of polysaccharides can be used to analyze the conformation of the sugar chain of polysaccharides, the connection mode of adjacent monosaccharide groups, and methylation.
- Determination of molecular weight
Gel permeation and mass spectrometry (MS) are the main methods for analyzing the molecular weight of polysaccharides, and the molecular weight of polysaccharides can be calculated by making linear equations with the spectra obtained.
- Monosaccharide composition
Thin layer chromatography (TLC), paper chromatography (PC), and high-performance liquid chromatography (HPLC) allow precise determination of the monosaccharide composition and molecular ratios of polysaccharides.
Here, the specific marine microbial polysaccharide production services we provide to our clients include but are not limited to:
Lipopolysaccharide Production Service
CD BioGlyco provides a one-stop solution for the extraction, purification, and characterization of lipopolysaccharides based on world-leading carbohydrate production technology.
Peptidoglycan Production Service
CD BioGlyco utilizes cutting-edge marine polysaccharide purification technology to extract, isolate, and refine peptidoglycan from marine microbial sources.
Capsular Polysaccharide Production Service
CD BioGlyco extracts and purifies capsular polysaccharides from marine microorganisms, and our services meet the research needs of a variety of industries, including food and pharmaceuticals.
Publication Data
Technology: HPLC, ultraviolet spectrum (UV), IR
Journal: Marine Drugs
IF: 5.4
Published: 2022
Results: In this publication, the authors extracted a novel polysaccharide (AUM-1) from marine Aureobasidium melanogenum SCAU-266 and characterized it by using techniques such as HPLC, UV, and IR. The molecular weight of AUM-1 was found to be 8000 Da. The major monosaccharide component of AUM-1 was glucose (97.3%) and the trace monosaccharides were mannose (1.9%) and galactose (0.8%), respectively. After methylation analysis, the authors found that 1,4-linked glucose residues were the major component of AUM-1, with a few residues being mannose residues, and the potential linkage type being 1,2-linked mannose residues.
Fig.1 Characterization of AUM-1 (a: HPLC plot. b: UV spectrum. c: molecular weight determination. d: monosaccharide composition analysis. e: IR plot). (Lin, et al., 2022)
Applications
- Marine microbial polysaccharides have good anti-tumor activity, so they can be used effectively in the development of anti-tumor drugs in biomedicine.
- Marine microbial polysaccharide has the potential as a stabilizing and emulsifying agent, and it can be used in the light industry.
- Marine microbial polysaccharide is often used as an ingredient in the production of cosmetics due to its excellent moisturizing properties.
Advantages
- We use advanced methods to extract, purify, and characterize marine microbial polysaccharides and these techniques ensure the high purity of the polysaccharides we produce.
- Depending on the different types of marine microbial polysaccharides, we choose the most appropriate extraction and characterization techniques.
- In addition to a one-stop solution for the production of marine microbial polysaccharides, we also provide our clients with detailed reports and analyses.
Frequently Asked Questions
What is the structure of the microbial polysaccharide?
Microbial polysaccharides are linear hetero-polysaccharides consisting of three to seven different monosaccharides arranged in groups of 10 or fewer to form repeating units. The monosaccharides may be pentoses, hexoses, amino sugars, uronic acids, etc.
What are the biological activities of marine microbial polysaccharides?
Marine microbial polysaccharide has good anti-tumor activity, immune-enhancing activity, antioxidant activity, and free radical scavenging activity.
CD BioGlyco produces marine microbial polysaccharides on a large scale and we manufacture marine polysaccharides with high purity. In addition to providing our clients with professional marine polysaccharide production solutions, we also have a wide range of marine polysaccharide products. Please feel free to contact us at any time.
Reference
- Lin, Y.Q.; et al. Ferroptosis-related immunomodulatory effect of a novel extracellular polysaccharides from marine fungus Aureobasidium melanogenum. Marine Drugs. 2022, 20(5): 332.
