Empowering Dehydrocrambine A Production Through Cutting-edge Technologies at CD BioGlyco
CD BioGlyco boasts extensive experience in the scalable production of Marine Biomolecules, such as Marine Carbohydrates, Marine Glycoproteins, Marine Lectins, Marine Glycosides, and Marine Glycolipids. The marine sponges belonging to the genus Monanchora have been definitively established as prolific reservoirs of novel secondary metabolites and show an array of potent biological activities. Predominantly among these metabolites are the guanidine alkaloids, which have been isolated from various Monanchora species. These compounds exhibit a wide spectrum of biological functions encompassing cytotoxic, apoptosis-inducing, antifungal, antibacterial, antiviral, antiprotozoal, antimalarial, and antitumor properties. Our researchers utilize abundant marine resource Monanchora species as raw material for production, harnessing their rich biodiversity to explore the potential of novel bioactive compounds. Through meticulous extraction methods such as aqueous extraction followed by sophisticated chromatographic techniques, we isolate valuable secondary metabolites, dehydrocrambine A.
Pretreatment of raw materials
We will obtain Monanchora sp. as raw materials and then conduct aqueous extraction of the sponge, followed by lyophilization and successive extraction with CH2Cl2-MeOH (1:1) and MeOH. The combined organic extracts will be evaporated under a vacuum and stored at -30°C.
Extraction and isolation
We partition the crude organic extract between EtOAc and H2O to obtain organic and aqueous extracts. The water-soluble extract will be fractionated using column chromatography.
Purification
The water-soluble extract will undergo chromatography on a C-18 column with increasing MeOH concentrations. Reversed-phase high-performance liquid chromatography is utilized to purify further to obtain the final product.
Additionally, we employ various techniques for comprehensively characterizing chemical structures and purity, ensuring accurate identification of the purified compounds.
- Two-dimensional nuclear magnetic resonance (2D NMR): This technique will be used to obtain detailed information about the connectivity of atoms within a molecule. By revealing correlations between pairs of nuclei in different environments, molecular structures will be determined. Besides, we use NOESY NMR and ROESY NMR spectroscopy to detect through-space interactions between protons that are close to each other in space, confirming their molecular conformation and relative stereochemistry.
- Mass spectrometry (MS): We utilize this technology to measure the mass-to-charge ratio of ions to know the molecular weight and structural features of dehydrocrambine A.
- Ultraviolet (UV) spectroscopy: Use UV spectroscopy to measure the absorption of light in the UV range by molecules for analyzing the conjugated systems and functional groups.
- Infrared (IR) spectroscopy: IR spectroscopy will also be applied to identify functional groups based on their absorption of infrared radiation. It is useful for determining the presence of specific bonds and groups within a molecule.
By comprehensively analyzing these parameters, we can ensure the high quality of our products and earn client’s confidence.
Publication Data
Journal: Nutrients
Published: 2018
IF: 5.9
Results: In this study, the authors comprehensively review the chemodiversity and biological activities of pyrroloquinoline and guanidine-derived alkaloids isolated from marine sponges belonging to Batzella, Crambe, and Monanchora genera, along with New Caledonian starfishes. Spanning the period from 1981 to 2017, the review focuses on polycyclic guanidinic compounds and their biomimetic potential. It underscores these genera as abundant sources of novel compounds with promising applications in cancer and other therapeutic areas. The authors mention dehydrocrambine A, a bicyclic compound derived from Monanchora sp., for its remarkable efficacy in inhibiting HIV-1 fusion. This compound underscores the significant biological potential discussed in the study. The article consolidates current knowledge in marine natural product chemistry and highlights the significance of these compounds for pharmacological research and natural product chemistry globally.
Applications
- Dehydrocrambine A can be used to research and develop novel targets for antiretroviral therapy development.
- Dehydrocrambine A can be utilized for the development and investigation of more potent HIV fusion inhibitors by structural modifications that enhance its efficacy or bioavailability.
- Dehydrocrambine A can be researched to investigate its potential for other biological activities, as compounds from marine sources typically exhibit multiple bioactivities.
Advantages
- Our researchers utilize abundant marine resource Monanchora species as raw material for production, harnessing their rich biodiversity to explore the potential of novel bioactive compounds.
- We employ diverse methods including aqueous extraction, lyophilization, and chromatography to isolate and purity dehydrocrambine and achieve high accuracy and purity.
- Our research team ensures product quality through the comprehensive characterization of chemical structures and purity, utilizing advanced spectroscopic techniques to provide a detailed product analysis report.
Frequently Asked Questions
What is the molecular formula and molecular weight of dehydrocrambin A?
The molecular formula of dehydrocrambin A is C24H42N6O2 and its molecular weight is 446.6 g/mol.
What services does CD BioGlyco offer for the application of carbohydrates?
CD BioGlyco possesses a professional research team with cutting-edge methodologies and extensive domain expertise, seamlessly catering to the exacting standards of the pharmaceutical, biotechnology, and research domains. Through state-of-the-art technologies, we ensure a steadfast and efficacious production process, promising an unwavering commitment to precision and reliability. If you want to pioneer advancements in research and propel the frontier of therapeutic innovation, please feel free to contact us!
Reference
- El-Demerdash, A.; et al. Batzella, Crambe and Monanchora: highly prolific marine sponge genera yielding compounds with potential applications for cancer and other therapeutic areas. Nutrients. 2018, 10(1): 33.
