Chitosan-based Drug-Delivery Vehicle Development Service

Chitosan-based Drug-Delivery Vehicle Development Service at CD BioGlyco

Chitosan also known as amino-polysaccharide, is mainly divided into three categories, namely α-chitosan, β-chitosan, and γ-chitosan. Chitosan is a polysaccharide obtained by partial deacetylation of chitin under alkaline conditions, which is composed of glucosamine and N-acetylglucosamine arranged alternately. Chitosan has a variety of biological properties and pharmacological activities and is used in drug delivery in various forms. Based on this, CD BioGlyco provides flexible chitosan-based drug delivery vehicle development service.

• Preparing chitosan solution: We add chitosan powder into suitable solvents (such as acetic acid and ethanol) and fully dissolve it under appropriate temperature and stirring conditions to form a chitosan solution.
• Adjust pH: Depending on the desired drug delivery properties, we add corresponding bases or acids to adjust the pH of the chitosan solution to achieve ideal drug vehicle properties.
• Encapsulate drugs: We add the target drug into the chitosan solution and use appropriate stirring or ultrasonic treatment to fully disperse and adsorb the drug on the chitosan molecules.
• Prepare multiple types of delivery vehicles
  • Nanoparticles: We add a cross-linking agent to the chitosan solution, then add the chitosan solution dropwise into the non-solvent, we prepare chitosan nanoparticles through the mixing and evaporation of the solvent. The nanoparticles in the solvent are then centrifuged and washed multiple times with an appropriate solvent to remove impurities and unencapsulated drugs. Finally, the nanoparticle deposits are dried at low temperatures to remove the remaining solvent.
  • Microspheres: We add chitosan solution dropwise into the cross-linking agent solution so that the chitosan forms a gel state under the action of the cross-linking agent. Common cross-linking agents include ionic cross-linking agents (such as ammonium sulfate) and chemical cross-linking agents (such as glutaraldehyde). The gel is then converted into tiny spherical structures by stirring or sonication and washed to remove impurities or uncross-linked chitosan.
  • Thin film: We use solution casting, solution spin coating, and spray coating methods to prepare the film, and solidify the chitosan film through drying and light curing.
  • Hydrogel: We make chitosan form a three-dimensional network structure hydrogel through a solution cross-linking reaction. According to specific needs, the hydrogel is then subjected to subsequent processing steps, such as cutting, shaping, freeze-drying, etc., to obtain the required vehicle form and properties.
• Characterization and analysis: We provide comprehensive characterization and analysis for various types of prepared chitosan vehicles, including evaluation of particle size, morphological structure, drug encapsulation efficiency, drug release characteristics, stability, etc. Chitosan vehicles control the release rate and duration of drugs and reduce frequent administration.

Fig.1 Chitosan-based drug-delivery vehicle development service. (CD BioGlyco)

Publication

Technology: Lyophilization

Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects

IF: 5.518

Published: 2019

Results: The authors prepared peptide-functionalized hybrid chitosan nanoparticles (NPs) encapsulating paclitaxel (PTX) and studied its use for targeting and treating overexpressing human breast cancer. The results showed that the peptide-functionalized nanoparticles had good pH and temperature sensitivity. Under the weakly acidic pH and higher temperature conditions of tumors, the drug rapidly released and effectively inhibited the growth of breast cancer cells. This indicated that peptide-functionalized nanoparticles had potential applications in targeted delivery and controlled release of anticancer drugs.

Fig.2 Peptide-functionalized chitosan NPs. (Qian, et al., 2019)

Applications

• Chitosan nano gels or films as drug delivery systems will promote wound healing and tissue regeneration.
• The drug is fixed in chitosan microspheres or nanoparticles to achieve slow and sustained release of the drug, reducing the frequency and dosage of medication.
• The preparation of chitosan nanoparticles or gels is applied to deliver various types of drugs and target them to different systems, such as lungs, eyes, brain, and other delivery systems.

Advantages

• CD BioGlyco has deep professional knowledge and rich experience in the field of chitosan and provides clients with professional chitosan-based drug delivery vehicle development and Tissue Scaffold Development services.
• CD BioGlyco uses advanced technologies and methods in the field of chitosan drug delivery, including nanotechnology, material modification, etc., to develop more efficient and stable drug delivery systems.
• Our technical team provides comprehensive chitosan-based materials and drug-delivery solutions to improve drug-delivery effects.

CD BioGlyco provides custom chitosan-based drug delivery vehicle development services to meet specific application needs. Please feel free to contact us if you need high-quality Marine Carbohydrate-based Drug-Delivery Vehicle Development Service, we look forward to cooperating with you.