Marine Glycoconjugate Profiling

Marine Glycoconjugate Profiling Service at CD BioGlyco

The marine environment is rich in a wide variety of biological species that produce glycoconjugates with enormous structural and functional diversity. Performing marine glycoconjugate profiling provides insights into their structure, composition, and functions to reveal their importance in biology, drug development, and ecosystems. As a leading global biotechnology company, CD BioGlyco provides diversified marine glycoconjugate profiling services.

• Lectin histochemistry: Lectin histochemistry is a commonly used technique that is used to detect the distribution and expression of marine glycoconjugates. The specific steps are as follows.
  • Sample processing: We pre-process the samples that need to be analyzed, such as crushing, centrifugation, or freezing to maintain the integrity and stability of the samples.
  • Fix the sample: We use 4% acetaldehyde solution or other appropriate fixative to fix the sample on the glass slide.
  • Antigen dehydration: We use appropriate dehydration methods, such as thermal dehydration, enzymatic dehydration, or acid dehydration, to expose the sugar groups in the sample for binding to lectins.
  • Lectin labeling: We select appropriate lectin probes (such as peanut agglutinin (PNA)) for labeling based on the affinity and specificity of the target sugar moiety. Lectins are often combined with fluorescent dyes or enzymes (such as horseradish peroxidase) to facilitate subsequent visualization and detection.
  • Lectin staining: We add labeled lectin probes to the samples on the slides and perform immunohistochemical staining to allow the lectins to specifically bind to the glycoconjugates in the samples.
  • Washing: We wash the sample on the slide with an appropriate buffer to remove non-specifically bound lectins or other impurities.
  • Observation and profiling: We observe the staining results using a microscope, and perform image analysis and quantitative processing of the observed staining results to obtain information about marine glycoconjugates.
• Microarray marine glycoconjugate profiling: We focus on microarray marine glycoconjugate profiling, a highly efficient analytical method to systematically determine the composition and relative abundance of marine glycoconjugates in a variety of samples. Microarray technology requires only small amounts of analytes and provides a miniaturized, high-throughput screening solution for the simultaneous analysis of multiple glycoconjugates.
• Omics profiling: For complex marine glycoconjugates, we also combine omics technology to provide a more comprehensive marine glycoconjugate profiling service.
  • Proteomic profiling: We extract and enrich proteins, obtain glycoprotein complexes from samples, and use mass spectrometry for protein identification and quantification. Proteomics provides information on the composition, structure, and interactions of marine glycoprotein complexes.
  • Glycomic profiling: We use a variety of techniques, such as high-performance liquid chromatography (HPLC), mass spectrometry, etc., to conduct qualitative and quantitative analysis of sugar molecules to understand the type, modification, and distribution of sugar molecules in marine glycoprotein complexes.

CD BioGlyco provides comprehensive profiling and identification for complex marine glycoconjugates, and we also provide specialized Characterization for known or isolated marine glycoconjugates.

Fig.1 Marine glycoconjugate profiling service. (CD BioGlyco)

Publication

Technology: Lectin histochemical methods

Journal: Zoological Science

IF: 0.955

Published: 2002

Results: The authors analyzed glycoconjugates in adrenocorticotropin (ACTH) and melanotropin (MSH) cells in the pituitary of adult sea lampreys by lectin histochemistry. The authors tested a total of 19 lectins, the results showed that ACTH cells (Figure 2) were positively stained by N-acetylglucosamine-binding agglutinin, N-acetylgalactosamine-binding lectin, and D-mannose-binding lectin after anti-lamprey NHF staining. MSH cells (Figure 3) were stained with anti-lamprey MSH-B and showed positive staining for N-acetylgalactosamine-binding agglutinin, D-mannose-binding lectin, and D-galactose-binding lectin. These results indicated that ACTH and MSH cells produced different types of glycoconjugates.

Fig.2 Agglutinin staining results in ACTH cell. (Ominato & Nozaki, 2002)

Fig.3 Agglutinin staining results in MSH cell. (Ominato & Nozaki, 2002)

Applications

• Disease Research: Analysis of marine glycoconjugates in disease samples reveals relationships with disease onset and progression, which helps develop new disease markers and provides strategies for disease research.
• Glycobiology: Marine glycoconjugates are important targets for glycobiology research. By analyzing marine glycoconjugates in different biological samples, we understand their structure, composition, and change patterns, thereby revealing the functions and regulatory mechanism of sugar in organisms.
• Vaccine Development: Marine glycoconjugates are of great importance in vaccine development. By analyzing marine glycoconjugates in vaccines, their immunogenicity, antigen specificity, and immunostimulatory effects are evaluated, guiding vaccine design and optimization.

Advantages

• Comprehensive profiling service: CD BioGlyco provides comprehensive profiling for marine glycoconjugates, including sugar structure identification, sugar modification analysis, quantitative determination, and interaction studies.
• High sensitivity and resolution: Our analytical methods offer high sensitivity and resolution, enabling the detection of low concentrations of marine glycoconjugates and the resolution of complex sugar modification patterns.
• High-quality result reports: We provide clear and detailed analysis results reports, including experimental processes, data analysis, and conclusions, to provide support for clients' further research and decision-making.

CD BioGlyco has the most advanced analysis and detection technology, which provides efficient and accurate profiling for marine glycoconjugates. In addition to simple qualitative and quantitative analysis, we also perform in-depth analysis to reveal the structural characteristics, spatial configuration, and biological functions of marine glycoconjugates. Please feel free to contact us if you would like to inquire about detailed content.