Unleashing the Power of Marine Glycosides: CD BioGlyco's Frondoside Production for Cutting-Edge Cancer Research
CD BioGlyco offers specialized frondoside production services, focusing on the extraction and purification of frondoside A, B, and C from the Atlantic sea cucumber Cucumaria frondosa. These triterpenoid glycosides exhibit potent anticancer properties, particularly frondoside A, which has shown significant antiproliferative, anti-invasive, and anti-angiogenic effects against various cancers, including pancreatic, breast, lung, and prostate cancers, as well as leukemia. Our advanced extraction, purification, and characterization techniques enable us to deliver consistent and reliable Marine Glycosides, supporting groundbreaking research in cancer treatment and other biomedical fields.
Frondoside A has a molecular mass of 1334 Da, a sulfate group on the first sugar residue, an aglycon ring with xylose in the third monosaccharide residue, a terminal monosaccharide residue of 3-O-methylglucose, and an acetoxyl group at C-16 in the aglycon ring. Frondoside B is a di-sulfated compound with two sulfate groups, while frondoside C is tri-sulfated with three sulfate groups and a nonholostane aglycone. The backbones of frondosides A, B, and C consist of polar saccharide chains, including hexose, pentose, or uronic acid, coupled with a non-polar (fat-soluble) aglycone. These saccharide chains comprise one or more linear oligosaccharides of 2-6 sugar units.
Fig.1 The structures of frondoside A, B, and C. (Al Shemaili, et al., 2016)
To produce and purify frondosides A, B, and C from the sea cucumber C. frondosa, CD BioGlyco employs a comprehensive process that includes extraction, fractionation, purification, characterization, and quality control. Here are the detailed steps involved. Moreover, we also offer comprehensive Marine Biomolecule Production solutions for clients worldwide.
Extraction
The blended tissues are processed with a solvent combination of chloroform, ethanol, and water for extraction. This process helps to solubilize the glycosides and other organic compounds present in the tissue.
Fractionation
The resulting crude extract undergoes fractionation to isolate the glycoside-rich components. This is often done using techniques like liquid-liquid extraction or preliminary chromatography to concentrate the glycosides.
Purification
The eluting fractions are collected and monitored using thin-layer chromatography (TLC) to identify and isolate the desired glycosides.
Characterization
Nuclear magnetic resonance (NMR) spectroscopy: NMR spectroscopy is employed to analyze the structure of the extracted glycosides, ensuring their chemical composition and purity.
Mass spectrometry: This technique is employed to determine the molecular weight and structural features of the glycosides, ensuring the correct identification of Frondosides A, B, and C.
Quality Control
High-performance liquid chromatography (HPLC) or other appropriate analytical techniques are used to evaluate the purity of the isolated frondosides.
Publication
Technology: Cell viability assays, MTT assays, Cell cycle analysis, In vivo mouse models
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: Frondoside A demonstrates broad-spectrum anti-cancer effects, including the induction of apoptosis, inhibition of cancer cell growth, migration, invasion, metastasis formation, and angiogenesis. These effects are attributed to its potent inhibition of p21-activated kinase 1 (PAK1), which is up-regulated in many cancers. Additionally, the study explored the molecular mechanisms underlying frondoside A's actions, revealing significant changes in gene expression related to cell cycle control and apoptosis, as well as its synergistic effects with conventional chemotherapy drugs.
Applications
- Frondosides, particularly frondoside A, are extensively studied for their potent anticancer effects, including the induction of apoptosis, inhibition of cancer cell proliferation, migration, invasion, and angiogenesis.
- Frondosides are used in research to identify biomarkers for response to treatment in cancer therapy.
- Frondosides can be used to study molecular pathways involved in cancer.
Advantages
- CD BioGlyco ensures high-purity frondosides through rigorous purification processes, including flash column chromatography and HPLC, which are crucial for accurate research results.
- CD BioGlyco guarantees the structural integrity and identity of the frondosides, providing reliable compounds for research applications.
- From extraction to characterization, CD BioGlyco offers comprehensive solutions, ensuring a seamless experience for researchers and developers.
Frequently Asked Questions
What types of frondosides does CD BioGlyco produce?
CD BioGlyco specializes in the production of frondosides A, frondosides B, and frondosides C, each with unique biological activities and potential therapeutic applications.
Can CD BioGlyco customize the production process for specific research needs?
Yes! CD BioGlyco offers tailored production services to meet specific research requirements, ensuring that the frondosides produced meet the desired specifications for purity, quantity, and bioactivity.
CD BioGlyco ensures that the production process of frondosides is conducted under stringent quality control measures, guaranteeing high-purity compounds suitable for research use. Please do not hesitate to contact us for more details about our service and feel free to get in touch for your solution.
References
- Al Shemaili, J.; et al. Pharmacokinetics in mouse and comparative effects of frondosides in pancreatic cancer. Marine Drugs. 2016, 14(6): 115.
- Adrian, T.E.; Collin P. The anti-cancer effects of frondoside A. Marine drugs. 2018, 16(2): 64.
- From Wikipedia: https://en.wikipedia.org/wiki/Orange-footed_sea_cucumber#/media/File:Cucumaria_frondosa_184708776_2.jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/Liquid%E2%80%93liquid_extraction#/media/File:Funnels.jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/Column_chromatography#/media/File:Column_chromatography_sequence.png.
- From Wikipedia: https://en.wikipedia.org/wiki/Thin-layer_chromatography#/media/File:TLC_black_ink_(cropped).jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Agilent1200HPLC.jpg.
