Laminarin-based Vaccine Development Service

Laminarin-based Vaccine Development Service at CD BioGlyco

Laminarin is a β-1-3-glucan derived from seaweed and consists of β(1→3)-glucans with β(1→6) branches. It has immunomodulatory, and radioprotective effects as well as anticancer activity. Thus, laminarin is a promising immunostimulatory molecule. At CD BioGlyco, we provide one-stop laminarin-based vaccine development services to enterprises, universities, and scientific research institutes around the world.

Extraction and Analysis of Laminarin

Laminarin is a polysaccharide extracted from the marine organism seaweed. At CD BioGlyco, we offer a variety of methods to extract laminarin, such as solvent extraction, auxiliary extraction, and combined extraction.

Next, we analyze its physicochemical properties and antitumor activity:

• Using a water bath dissolution assay to determine the solubility of laminarin.
• Using Congo red assay to analyze the presence of three-stranded helical structure of laminarin.
• Using ultraviolet spectroscopy to analyze the presence of proteins and nucleic acids in laminarin.
• Using 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay to determine the inhibition rate of laminarin on tumor cells.

Development of Vaccines

• Chemical method
  First, we construct the carrier protein and express and purify it. Second, we use chemical reagents to activate laminarin and protein separately and crosslink them in vitro. Finally, the cross-linking product is purified to obtain the vaccine product.
• Biological method
  This is a common method for developing vaccines. First, we construct an engineered strain. Then, we construct an expression vector containing the laminarin synthesis gene cluster, vector protein, and glycosyltransferase. Next, we transfer the laminarin to the carrier protein and purify it to obtain the glycoprotein after induction and expression. In this process, the production of vaccine is mainly accomplished by the induction and expression of the engineered strain and further purification.

Fig.1 Chemical and biological processes of vaccine preparation. (CD BioGlyco)

In addition, we provide in vivo and in vitro vaccine bioactivity screening services to further enhance vaccine activity. These include humoral and cellular immunity tests, among others.

Fig.2 Laminarin-based vaccine development services. (CD BioGlyco)

Publication

Technology: Western blotting, Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis

Journal: Frontiers in Marine Science

IF: 3.7

Published: 2023

Results: This research intends to investigate the immunomodulatory effects of laminarin on macrophages. After experiments as well as RT-qPCR analysis, and western blotting, it was found that 100 μg/mL of laminarin enhanced phagocytosis in mice after incubation for 4 h. Laminarin was able to induce intelectin (MaINTL) in primary hepatocytes and macrophages, and recombinant MaINTL ( rMaINTL) can bind to laminarin. In addition, laminarin was able to induce the expression of genes related to cytoskeletal remodeling in primary cells. These findings suggest that laminarin enhances the phagocytic capacity of immune cell macrophages. Macrophages increased their phagocytic capacity through MaINTL activation and up-regulation of downstream cytoskeletal remodeling-related gene expression. Thus, laminarin may act as an immune booster to enhance host immunity and resistance to disease. Meanwhile, it also makes a basis for the development of immunogenic vaccines.

Fig.3 Fucoidan and laminarin increase the phagocytic capacity of macrophages against FITC-labeled A. hydrophilus. (Li, et al., 2023)

Applications

• The development of laminarin-based vaccines can be used to research immunomodulatory mechanisms in the human body.
• The development of laminarin-based vaccines can be used to develop polysaccharide expression and facilitate the realization of artificial novel design and synthesis of polysaccharides.
• The laminarin-based vaccine development process can be used to investigate the immunization mechanism of polysaccharide conjugate vaccines.

Advantages of Us

• Our R&D team is experienced in the preparation and development of diverse marine carbohydrates and their associated Vaccines and offers a large specification and specialized custom synthesis service.
• Each of our experimental protocols as well as our products undergoes rigorous quality testing to ensure the high quality and quality of our products.
• Our pre-and post-sales technical support is provided by experienced and skilled scientists to ensure client satisfaction.

CD BioGlyco specializes in research in the field of glycobiology with many years of development and extensive industry experience. We provide not only the development of carbohydrate vaccines but also the Modification of Marine Carbohydrates and the synthesis of Marine Carbohydrate Adjuvants. We hope to be your reliable partner in your R&D work. Please feel free to contact us if you would like to know more about this.