Hyaluronic Acid-based Mimetic Development Service

Hyaluronic acid (HA) is a unique linear macromolecular acidic mucopolysaccharide composed of the disaccharide units of glucuronic acid (GlcA) and N-acetyl glucosamine (GlcNAc). Notably, HA fragment mimics of different sizes have special biological functions. CD BioGlyco provides customized mimetic design and synthesis services according to the different carbohydrates such as Sulfated Fucan, Sulfated Galactan, Heparin, Alginate, and HA. We provide HA-based mimetics design, synthesis, identification, and activity analysis services. We help clients explore the application and research of Marine Carbohydrate Mimetics in cytobiology, pathology, immunology, and biological tissue engineering.

Fig.1 HA synthesis and structure. (Amorim, et al., 2021)

HA-based Mimetic Development Service at CD BioGlyco

• Design and Synthesis of HA-based Mimetic
  Based on the structural properties of HA, our researchers provide in vitro synthesis strategies and chemical assembly strategies to help clients develop HA-based mimetics quickly and efficiently.
  • In Vitro Synthesis Strategy
    CD BioGlyco provides two in vitro synthesis strategies of homogeneous HA-based mimetic and one-pot multienzyme (one-potful enzyme, OPME) strategy. We realize the preparation of HA-based mimetics by using a multi-cascade reaction system, catalytic coupling, and polymerization.
  • Chemical Synthesis Strategy
    Our synthesis group efficiently synthesizes HA-based mimetics through different building blocks and selective mobile protection.

• Bioactivity Assessment Service of HA-based Mimetics
  Our researchers provide high-throughput screening and identification services to evaluate HA-based mimetic-specific binding and interacting proteins at the cellular level with the aid of glycoproteomics technology to support the study of the biological functions of HA-based mimetics. We provide systematic analytical services on the mechanism of action of HA-based mimetic of different molecular weights, which include not only the assessment of the biological activity of HA-based mimetic [e.g., interactions with different proteins, such as growth factors (GFs), but also the mechanism of regulation of HA-based mimetic with the extracellular matrix. In addition, for 3D modeling of HA-based mimetics, we also provide corresponding disease modeling and analysis services.

Fig.2 Flow chart of HA-based mimetics development services. (CD BioGlyco)

Publication

Technology: Agarose gel electrophoresis, Nuclear magnetic resonance (NMR), Electrospray ionization mass spectrometry (ESI-MS)

Journal: Chemical Communications.

IF: 6.319

Published: 2017

Results: This article reports a strategy for homogeneous HA conjugate synthesis catalyzing HA chain formation by P. multocida (PmHAS) coupling. All synthetic targets need only be purified using gel filtration. The obtained targets were characterized by ESI-MS and NMR. Analysis by thin-layer chromatography and capillary electrophoresis showed that HA oligosaccharides rapidly initiate PmHAS-HA-catalyzed polymerization. Notably, multiple validations and a good linear relationship of molecular weight-acceptor/donor molar ratio indicate the universality of this strategy. Fluorescence profiles by combining HA-CD44 interaction and biotin-avidin-specific recognition indicate that exogenous HA conjugates can efficiently aggregate on the cell surface or inside the cell.

Fig.3 Biological application of homogeneous HA-biotin conjugates. (Fu, et al., 2017)

Applications of HA-based Mimetic

• HA-based mimetics act as an aggressive and metastatic modulator involved in the development of pathological states in cancer.
• HA-based mimetics interfere with and block the proliferation of breast cancer cells by activating the Hippo pathway.
• HA-based mimetics is applied in the medical health field as an inert filling material.
• As a drug carrier, HA-based mimetics plays a key role in drug delivery, drug thickening and dilution, etc.
• HA-based mimetics are associated with tumorigenesis and can be used as a new target for intervention in tumor therapy.

Advantages of Us

• We provide gram-scale analog preparation services to facilitate the biological function of HA-based mimetics.
• To improve the efficiency of in vitro synthesis, our researchers have repeatedly optimized the synthesis reaction system to ensure the best synthesis route for our customers.

CD BioGlyco is recognized for providing high-quality Marine Biomolecule production services to clients in many countries. Our company has over twenty years of industry experience. Moreover, we provide reliable Marine Carbohydrate-based Biomaterial Engineering Development services. For more detailed information please feel free to contact us .