Experience the Excellence of Marine Bioactives with CD BioGlyco's Aurantoside K Production!
At CD BioGlyco, we provide high-quality and trailed aurantoside K production service based on our Marine Biomolecule Production Services. Aurantoside K is sourced from a marine sponge belonging to the genus Melophlus, this particular sponge was collected from the Fiji Islands, highlighting the region's rich biodiversity and its potential for yielding valuable bioactive compounds. Aurantoside K is characterized by its bright orange color and amorphous solid state. Its structural integrity and composition have been confirmed through various sophisticated spectroscopic techniques, including 1H nuclear magnetic resonance (NMR), 1H-1H correlation spectroscopy (COSY), heteronuclear single-quantum correlation spectroscopy (HSQC), and heteronuclear multiple-bond correlation spectroscopy (HMBC). Notably, Aurantoside K demonstrates significant antifungal properties, showcasing the importance of marine organisms as a reservoir of potent natural products. Additionally, we offer several other Marine Glycoside Production services for clients to speed up their research on glycoscience.
Fig.1 The key 1H-1H COSY and HMBC correlations of aurantoside K. (Kumar, et al., 2012)
Here are the details of our process of aurantoside K production service from the sponge.
Collection and initial extraction
The collected sponge material is then extracted using methanol (MeOH) at room temperature. This extraction process helps dissolve the bioactive compounds present in the sponge. The resulting crude extract is partitioned between 90% MeOH and hexane, allowing for the separation of compounds based on their polarity.
Fractionation
The methanol fraction, which contains the target compounds, undergoes further fractionation. This is done using flash chromatography on an ODS-A column, a technique that separates compounds based on their interactions with the column material. Fractions displaying significant anti-candida activity are identified and pooled for further processing.
Purification
The pooled fractions are then purified through semi-preparative reverse-phase high-performance liquid chromatography (RP-HPLC). This purification process utilizes a Zorbax ODS column and a gradient of aqueous CH3CN with trifluoroacetic acid (TFA) to obtain pure aurantoside K.
Verification
The final verification of purity and structure involves advanced analytical techniques. High-resolution electrospray ionization mass spectrometry (HRESIMS) is used to confirm the molecular weight and purity of aurantoside K. Additionally, nuclear magnetic resonance (NMR) spectroscopy provides detailed information on the compound's structural integrity, ensuring that the isolated substance is indeed aurantoside K.
Publication
Technology: HRESIMS, 1H NMR, 1H-1H COSY, HSQC, HMBC
Journal: Marine Drugs
IF: 4.9
Published: 2012
Results: This compound originates from a marine sponge of the Melophlus genus, which was gathered from the Cicia region in the Lau group of the Fiji Islands. While aurantoside K did not exhibit significant effects in antimalarial, antibacterial, or cytotoxicity tests against HCT-116 human cancer cells, it did display a wide range of antifungal activities. It was particularly effective against a variety of fungal pathogens, such as amphotericin-resistant and wild-type Candida albicans, Cryptococcus neoformans, Aspergillus niger, Penicillium species, Rhizopus sporangia, and Sordaria species.
Applications
- Aurantoside K can be explored as a potential antifungal agent in the pharmaceutical industry, especially for creating treatments for infections caused by drug-resistant fungal strains.
- Researchers can utilize aurantoside K to study its mechanisms of action against various fungal pathogens, contributing to the broader understanding of antifungal agents and their applications.
- Due to its unique structure and biological activity, aurantoside K is an excellent candidate for drug discovery programs aimed at identifying new therapeutic agents for fungal infections.
Advantages
- CD BioGlyco leverages advanced membrane filtration techniques to ensure high purity and quality of aurantoside K, capitalizing on their expertise in marine polysaccharide purification.
- CD BioGlyco offers tailored purification processes that can be adjusted to meet specific client requirements, ensuring optimal results for diverse research and industrial applications.
- Aurantoside K exhibits a wide range of antifungal properties, making it a valuable compound for developing antifungal agents and treatments, particularly against resistant strains of pathogens.
Frequently Asked Questions
What is aurantoside K and why is it important?
Aurantoside K is a tetramic acid glycoside known for its significant antifungal properties. It is important due to its potential applications in pharmaceutical development as an antifungal agent, especially against drug-resistant fungal pathogens.
How does the production process at CD BioGlyco contribute to the sustainability of marine resources?
CD BioGlyco is committed to sustainable practices, ensuring that marine resources are responsibly sourced and utilized. We follow ethical guidelines and collaborate with local communities and researchers to minimize environmental impact while maximizing the potential of marine-derived compounds.
By choosing CD BioGlyco's aurantoside K production service, clients benefit from advanced purification technologies, expert knowledge, and customized solutions that ensure high-quality and efficient production of this valuable antifungal compound. Please feel free to contact us for more information, if you are interested in our services.
References
- Kumar, R.; et al. Aurantoside K, a new antifungal tetramic acid glycoside from a Fijian marine sponge of the genus Melophlus. Marine Drugs. 2012, 10(1): 200-208.
- From Wikipedia: https://upload.wikimedia.org/wikipedia/commons/2/20/Aplysina_archeri_%28Stove-pipe_Sponge-pink_variation%29.jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/Fractionation#/media/File:Differentielle_zentrifugation.png.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Reverse_Phase_Gradient_Elution_Schematic.svg.
