Marine Glycoenzyme Design Service

CD BioGlyco provides a full range of Marine Glycoenzyme Development Services to provide strong support for clients' projects. Our cutting-edge capabilities harness the significance of marine glycoenzyme design, driving innovation in biotechnology. By integrating advanced methodologies and in-depth knowledge, we meticulously craft custom-designed enzymes tailored to client's specific needs.

Marine Glycoenzyme Design Service at CD BioGlyco

Marine glycoenzyme mainly includes marine glycosyltransferase and marine glycoside hydrolase. It is found in marine organisms and plays a vital role in the cycling of carbon and nutrients in marine ecosystems. Through biological means, CD BioGlyco improves and optimizes the characteristics of marine glycoenzyme, as well as improves their catalytic efficiency and stability, thereby promoting the efficient production of carbohydrate compounds.

We provide three approaches for creating glycoenzyme: rational design, directed evolution, and semi-rational design.

Fig.1 General strategies for the design of glycoenzymes. (Cui, et al., 2022)

In rational design strategies, we utilize site-directed mutagenesis and existing sequences or crystal structures to devise new or enhanced functionalities. This strategy suits scenarios with a straightforward correlation between structure and function.

In directed evolution strategies, we imitate the rapid natural evolution of glycoenzyme by generating numerous potential variants through recombinant DNA technology, employing techniques like error-prone polymerase chain reaction or gene shuffling, and identifying them swiftly using high-throughput screening methods. This method is preferable for situations with a less apparent link between structure and function.

In semi-rational strategies, we combine the predictive elements of rational design with empirical-directed evolution to form a compact model. It relies on advancements in protein structure prediction systems, offering controlled diversity, limited mutations, and size-filtered libraries of specific random residues. Structure-guided directed evolution ensures a comprehensive exploration of critical residues while maintaining necessary throughput and cost-effectiveness.

Publication

Technology: Directed evolution of enzyme

Journal: Nature Chemistry

IF: 24.274

Published: 2016

Results: The authors reported the development of highly active and stereoselective transaminases originating from fold class I. The transaminases were painstakingly engineered and subsequently optimized based on the identified motif. The ensuing enzymes displayed up to 8,900-fold heightened activity compared to the starting scaffold and demonstrated high stereoselectivity (in excess of 99.9%) in the asymmetric synthesis of a series of chiral amines with bulky substituents. The authors also noted that the motif was successfully engineered into additional protein scaffolds with sequence identities dipping to 70%, reinforcing the potential wide-ranging impact on the biocatalytic synthesis and enzyme engineering fields.

Here are some of the results shown in this article:

Fig.2 Quinonoid of compounds accommodated in the active site of 3FCR. (Pavlidis, et al., 2016)

Applications of Marine Glycoenzyme Design

• Strategies for marine glycoenzyme design can be used to develop novel drugs. By simulating and analyzing the molecular structure and activity of glycoside hydrolase, its binding ability to drug molecules can be predicted and optimized, thereby discovering new drugs with therapeutic effects.
• Strategies for marine glycoenzyme design can be used to optimize existing glycoside hydrolases to improve their catalytic activity and stability. This helps speed up the synthesis and decomposition process of compounds.
• Strategies for marine glycoenzyme design can be used to develop therapies for certain diseases related to abnormalities in carbohydrate metabolism. For example, glycoside hydrolase inhibitors can be designed and optimized to treat diseases such as diabetes that are associated with abnormalities in carbohydrate metabolism.

Advantages of Us

• Our team is composed of experienced experts in marine biology, enzymology, and bioinformatics. We have deep professional knowledge and skills to provide you with high-quality marine glycoenzyme design services.
• We use computer-aided design technology to quickly and accurately predict and optimize the molecular structure and activity of marine glycoenzyme.
• We provide personalized service plans and tailor-made services based on clients' research fields, project characteristics, and time requirements.

With the support of professional development team, CD BioGlyco is committed to providing clients with a leading marine glycoenzyme design service to assist innovative research. Clients in need are welcome to contact us and let us open a new chapter together!