Your Best Partner for Marine Monosaccharide Analysis
The composition of glycans of marine glycoproteins has an impact on the maintenance of their conformation and stability, functions, and biological activity. Analysis of the monosaccharide composition of marine glycoproteins plays an important role in analyzing their constitutive relationships, and potential applications in medicine, etc. Based on extensive practical experience in Marine Glycoproteomics and state-of-the-art equipment, CD BioGlyco provides a high-quality and professional marine monosaccharide analysis service. We help clients identify glycosylation types and analyze glycoprotein structures by analyzing the monosaccharide composition of marine glycoproteins.
Marine Monosaccharide Analysis at CD BioGlyco
We use sensitive methods to analyze the monosaccharide composition of marine glycoproteins. With many years of experience in the Structural Analysis and research of marine glycoproteins, we achieve rapid characterization and quantification of monosaccharides. The marine monosaccharide analysis procedure is as follows:
- Sample pre-treatment: We pre-treated samples of different complex matrices of marine organisms to remove impurities.
- Extraction and purification of glycoprotein: Glycoproteins are extracted from the samples using appropriate techniques and purified by precipitation, dialysis, ion-exchange chromatography, etc.
- Monosaccharide analysis: After derivatization, the composition of monosaccharides is analyzed by high-performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS). The experimental data are processed to determine the type of monosaccharides in marine glycoproteins.
- Other analyses: In addition to the monosaccharide composition, we also combine nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), and other techniques to analyze the structure and amino acid composition of marine glycoproteins.
We customize our analytical protocols based on the sample type and research objectives to best meet our client's research needs. Based on standardized quality control criteria, experiments are reproducible and results are accurate and reliable.
Applicable Samples
Our marine monosaccharide analysis service applies to various tissues of different types of marine organisms such as algae, starfish, and fish. Upon receipt of the client's samples, we complete all experiments from glycoprotein extraction, and monosaccharide detection to analysis.
Results Delivery
We provide a detailed marine monosaccharides analysis report and offer valuable insights, mainly covering the following:
- Technical and experimental steps of the analysis
- Relevant mass spectrometry parameters
- Raw assay data
- Marine monosaccharide composition analysis results
Publication Data
Technology: Ion-exchange chromatography, GC-MS, FTIR
Journal: RSC Advances
IF: 3.119
Published: 2019
Results: In this study, the chemical composition, nutritional properties, and glycoproteins in Sardinella zunasi Bleeker were analyzed. The researchers extracted and purified glycoprotein (H2) from S. zunasi Bleeker and analyzed its monosaccharide composition, analytical amount, and immunomodulatory activity. The results showed that the average molecular weight of H2 was 7145 Da and the main monosaccharides were mannose and galactose. It has some immunomodulatory activity in a dose-dependent manner.
Fig.1 Spectroscopic analysis of H2. (Han, et al., 2019)
Applications
- Marine glycoproteins have potential pharmaceutical applications. The analysis of the monosaccharide composition of marine glycoproteins helps to gain insight into their functions and applications in medicine.
- Monosaccharide composition analysis of marine glycoproteins plays an important role in studying their applications in nutraceuticals and cosmetics.
- The analysis of the monosaccharide composition of marine glycoproteins helps in the in-depth evaluation of their biological activities.
Advantages
- We put our client's needs first and design comprehensive solutions for marine monosaccharide analysis.
- Based on our rich experience in marine glycoproteins analysis and professional technical support, we are ready to answer any questions arising from the analysis.
- Our well-established marine monosaccharide analysis process ensures that the project is carried out efficiently in order to provide the analysis results quickly.
Frequently Asked Questions
What is the biological significance of marine monosaccharides analysis?
Marine glycoproteins have diverse biological functions and are involved in intracellular or surface recognition, cell adhesion, signaling, and other processes. They are found in various marine organisms, but their glycosylation sites, molecular weights, and monosaccharide compositions vary. The analysis of monosaccharides in marine glycoproteins helps to understand their structures, and conformational relationships, and deeply analyze their biological functions and activities.
What are the advantages of different methods in analyzing the monosaccharide composition in glycoproteins?
- HPLC: This technique has the advantages of high resolution, fast separation speed, good separation effect, good reproducibility, etc. It is suitable for analyzing the monosaccharide composition of most substances.
- GC-MS: This method is commonly used to analyze the composition of monosaccharides in glycoproteins, which is determined by converting the monosaccharide components into volatile derivatives through chemical modification such as methylation. It is characterized by high sensitivity, rapid analysis, and high separation efficiency.
CD BioGlyco has a dedicated team that designs and conducts marine monosaccharide analysis programs. Welcome to contact us to discuss the detailed process of monosaccharide analysis. We look forward to exploring the mysteries of marine glycoproteins with you.
Reference
- Han, Y.; et al. Nutritional characteristics of marine fish Sardinella zunasi Bleeker and immunostimulatory activities of its glycoprotein. RSC advances. 2019, 9(52): 30144-30153.
