Quantification Analysis of Marine Glycoprotein

Quantification Analysis of Marine Glycoprotein at CD BioGlyco

CD BioGlyco has accomplished a series of research challenges with its rich experience in Glycoproteomics. As domestic and foreign researchers pay more attention to the development and utilization of marine resources, the study of glycoproteins derived from marine animals, etc., has gradually become a hot spot. It is found that marine glycoprotein has a variety of biological activities. The key to the study of marine glycoprotein in biology and disease diagnosis lies in the ability to quantitatively analyze it. We provide a one-stop service for quantitative analysis of marine glycoprotein.

Quantitative analysis services for protein include absolute or relative quantification. Our service is used for the analysis of different samples of cells, serum, tissues, and so on. The client just needs to tell us the purpose of their experiment. We will develop an exclusive quantitative analysis program according to the client's requirements.

Fig.1 Process of quantification analysis of marine glycoprotein. (CD BioGlyco)

Relative Quantification Analysis of Marine Glycoprotein

Relative protein quantification is a mass spectrometry-based technique. The purpose of relative quantification is to compare the relative abundance of proteins in each sample. By resolving differences in protein expression in organisms under different conditions, the role of proteins in various biological processes is clarified. We have a diverse range of isotope labeling quantification techniques, including isotope-coded affinity tags (ICAT), isobaric tags for relative and absolute quantitation (iTRAQ), etc. Combining mass spectrometry with labeling techniques, relative quantitative analysis of glycoproteomics is achieved by enzymatic cleavage, isotope labeling, separation, and detection of proteins.

With the in-depth study of glycosylation modifications, mass spectrometry-based methods for glycosylation analysis are greatly developed. Non-labeled mass spectrometry quantitative analysis methods also achieve relative quantitative detection of glycoproteins. We also provide a relative quantitative analysis of marine glycoprotein based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

Absolute Quantification Analysis of Marine Glycoprotein

Absolute quantitative analysis is a targeted quantitative proteomics technique. This technique is used for absolute quantification of proteins and their modification states. We use multiple reaction monitoring (MRM) or selective reaction monitoring (SRM) to determine the absolute content.

Publication Data

Technology: Two-dimensional difference in gel electrophoresis (2D-DIGE)

Journal: Scientific Reports

IF:4.997

Published: 2021

Results: In this study, manatee serum proteomics was analyzed using iTRAQ liquid Chromatography with tandem mass spectrometry (LC-MS/MS) and 2D-DIGE. By this method differentially expressed proteins in the serum of manatees affected by different mortality events were identified. The results showed increased levels of several proteins in the serum of manatees from the Indian River Lagoon compared to controls, including histidine-rich glycoprotein, among others. This study demonstrates that glycoproteomics analysis can be used to analyze disease-related proteomes.

Fig.2 Differential in DIGE separation of serum proteins. (Lazensky, et al., 2021)

Applications

• Quantitative analysis of marine glycoprotein is used in gene expression regulation studies. By comparing the differences in protein expression under different conditions, it is possible to study the post-transcriptional regulation and post-translational modification of gene expression.
• Quantitative analysis of marine glycoprotein is used to study sugar-related signaling pathways. By quantitatively analyzing the differences in glycoprotein expression, the role of different signaling molecules can be investigated, thus revealing the activation and inhibition mechanisms of signaling pathways.
• Quantitative analysis of marine glycoprotein can be used for comparative glycoproteomics studies. By comparing the protein expression differences between different species or subtypes, we will explore the evolutionary relationship between species, genetic variation, etc.

Advantages of Us

• We utilize efficient data processing steps and software for accurate and reliable quantitative analysis of marine glycoprotein.
• Our instrumentation is highly sensitive and capable of detecting low-abundance proteins.
• Our marine glycoprotein quantitative analysis services are widely applicable. It is suitable for a wide range of biological samples, including cells, tissues, and biological fluids, and is used to study a variety of biological processes.

At CD BioGlyco, our goal is to be the best partner for our clients' Marine Glycoprotein research. Please feel free to contact us for detailed information about marine glycoprotein quantitative analysis steps, quotes, sample requirements, and more. All you need to do is to provide your experimental requirements and samples. We will complete all the experimental operations such as sample processing, enzyme digestion, mass spectrometry detection, and so on.