Foot Protein 1 Production Service

Foot Protein 1 Production Service

Trust Our Foot Protein 1 Production for High-quality Products

The foot filaments of mussels contain different proteins such as foot protein 1. These proteins are well characterized for biomedical and technological applications, such as the development of bioadhesives, etc. CD BioGlyco offers a one-stop foot protein 1 production service from experimental design, and production to characterization. With our extensive experience in the production of Marine Glycoproteins and other Marine Biomolecules, we develop customized analytical protocols and produce high-quality foot protein 1 to ensure maximum satisfaction with our client's needs.

We mainly provide foot protein 1 production service in blue mussels. To improve the purity and yield of foot protein 1 and to maintain the structural integrity and biological activity, we have optimized the whole production process. The production process is as follows:

Extraction

The shell of the blue mussel is peeled off and the pigmented epithelium is removed. Then the foot is placed in a grinder, acetic acid and protease inhibitors are added and homogenized to extract foot protein 1 for subsequent purification and centrifugation.

Purification

We use centrifugation, dialysis, and chromatography to remove impurities and purify the extract. Gel filtration chromatography, size exclusion chromatography, and reversed-phase high-performance liquid chromatography (HPLC) are commonly used chromatographic methods to purify foot protein 1. After purification, the pure foot protein 1 is freeze-dried and ready for characterization and other studies.

Characterization

Characterization of foot protein 1 includes sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry, etc., to understand its structure, sequence, and other properties.

Flowchart of foot protein 1 production. (CD BioGlyco)

In addition to foot protein 1 from blue mussels, we produce other marine glycoproteins such as Mucin-type Glycoprotein, Stanniocalcin, Marine Collagen, etc. Our marine glycobiologists develop efficient production programs and provide research guidance.

Publication Data

Technology: Gel filtration, reversed-phase HPLC, mass spectrometry

Journal: Journal of Biological Chemistry

IF: 4.238

Published: 2009

Results: In this study, foot protein 1 was isolated, purified, and characterized from Perna viridis. It was purified in three steps: gel filtration, reversed-phase HPLC, and gel filtration. By characterizing its structure, the researchers found that foot protein 1 consists of at least two variants of 50 and 64 kDa, which have four different structural domains and highly repetitive sequences. This study provides methodological support for our extraction and characterization of foot protein 1.

Fig.1 Repeat structural domains of foot protein 1 are associated with consensus decapeptide repeat sequences. Fig.1 Repeated structural domains of foot protein 1 in Mytilus edulis and P. viridis. (Zhao, et al., 2009)

Applications

  • Blue mussel foot protein 1 has strong biocompatibility and adhesive properties. It is used in the development of biomaterials for tissue engineering, wound healing, and coatings.
  • Due to its extensive adhesion matrix and strong adhesion properties underwater, blue mussel foot protein 1 is used to study marine cementation devices.
  • Based on its good biological properties, blue mussel foot protein 1 is also used in the development of tissue adhesives, biosensors, and other applications.

Highlights

  • We respond quickly to client research objectives and develop tailored foot protein 1 production programs to ensure 100% client satisfaction.
  • We purify foot protein 1 through a series of steps including centrifugation, dialysis, gel filtration chromatography, etc., to ensure consistency.
  • We offer full characterization of the produced foot protein 1 and provide detailed analytical reports.

Frequently Asked Questions

What are the properties of blue mussel foot protein 1?

Blue mussel foot protein 1 is a protein derived from the adhesive foot of the blue mussel M. edulis. It has been widely studied for its high adhesive properties, a wide range of adhesion matrices, and biocompatibility, and has great potential for applications in biomedicine, materials development, etc. Foot protein 1 has also been a focus of research in adhesive development.

Why do we produce blue mussel foot protein 1?

Blue mussel foot protein 1 is a component of the protective cuticle and plays a crucial role in the unique adhesion properties of mussel foot filaments. The production of high-quality foot protein 1 is important for the in-depth understanding of glycoprotein-mediated adhesion in marine organisms and the development of artificial adhesion proteins.

Based on our extensive experience in marine glycoprotein production, CD BioGlyco ensures high-quality extraction, purification, and detailed foot protein 1 characterization services. Please feel free to contact us to tell us about your research goals, if you need foot protein 1 production. We will give you the best product and service experience.

Reference

  1. Zhao, H.; et al. Glycosylated hydroxytryptophan in a mussel adhesive protein from Perna viridis. Journal of Biological Chemistry. 2009, 284(35): 23344-23352.
For research use only. Not intended for any clinical use.
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