Probiotic Activity Screening Service at CD BioGlyco
Probiotics and their related products have important applications in most health products aquaculture and livestock farming. Marine glycans have become an important source of innovative probiotic development due to their unique probiotic activity. CD BioGlyco provides our clients with a professional yet reliable screening of marine glycans and glycoproteins for probiotic activity, based on our extensive experience in researching the Biological Activity of Marine Glycans & Glycoproteins.
We take the samples to be tested and culture them together with different and already activated strains, and at the same time, set up a blank control group. Then, the optical density value of the culture solution at around 600 nm, as well as the pH value, are detected using an ultraviolet-visible spectrophotometer. The probiotic activities of marine glycans and glycoproteins are analyzed based on the growth curves.
The related services we provide include but are not limited to the following.
Low pH Tolerance Assay
Different pH environments affect the probiotic activity of marine glycans and glycoproteins. Firstly, we test the low pH tolerance of marine glycans and glycoproteins. We use a hydrochloric acid solution to adjust the pH of the liquid medium to lower values for marine glycans & glycoproteins and specific bacteria, respectively. The glycans and glycoproteins to be tested are incubated with the specific bacteria, while a blank control group is set up. Finally, the optical density of each group is measured at 600 nm to analyze the low pH tolerance and bacterial inhibitory activity of the samples to be tested.
Bile Salt Tolerance Assay
We add bile salts to the broth culture separately to get different concentrations of bile salt solutions. The samples (marine glycans and glycoproteins) are to be tested and specific bacteria are added to them separately, mixed, and incubated. Meanwhile, a blank control group is set up. Finally, the absorbance of each group is measured to analyze the bile salt tolerance of the samples to be tested.
Antibiotic Susceptibility Assay
Antibiotic susceptibility testing is an important element in the in vitro safety assessment of existing and potential marine glycans and proteins. We offer a variety of experimental methods to test substances for antibiotic susceptibility. Examples include antimicrobial gradient strips, agar dilution methods, tube-dish diffusion methods, and a variety of polymerase chain reaction methods. Secondly, we also offer the concentration gradient test strip method to determine the lowest inhibitory concentration of the sample to be tested to distinguish between natural and acquired resistance.
Fig.1 Probiotic activity screening services. (CD BioGlyco)
Publication
Technology: Ulcerative colitis mouse model, High-throughput sequencing
Journal: Marine Drugs
IF: 5.4
Published: 2022
Results: In this research, the authors utilized a mouse model of dextrose sodium sulfate (DSS)-induced ulcerative colitis to characterize the beneficial properties of Enteromorpha clathrata polysaccharides (ECP). High-throughput sequencing and bioinformatics analyses showed that ECP significantly altered the structure of the intestinal microbiota and increased the abundance of Paramecium spp. Furthermore, the research found that Parabacteroides distasonis had an anti-ulcerative colitis effect on DSS-fed mice. In this research, the beneficial effect of ECP on ulcerative colitis was demonstrated for the first time.
Fig.2 Dietary ECP ameliorated DSS-induced mucosal damage in the colon. H&E staining (A) and Alcian blue staining (B) of the colon tissues. (Ma, et al., 2022)
Applications
- Probiotic activity screening for marine glycans and glycoproteins can be used to research treatments for antibiotic-associated diarrhea.
- Screening tests for probiotic activity can be used to develop treatments for patients with immune system deficiencies and intestinal defects.
Advantages of Us
- We offer custom probiotic activity screening services.
- Each of our testing protocols is quality tested.
- We offer protocols for the vast majority of marine glycans and glycoproteins.
- We have the world's leading glycan bioactivity assay technology to ensure that our clients' requirements are met.
CD BioGlyco has first-class laboratory equipment and experienced researchers to provide high-quality bioactivity assays for marine glycans and glycoproteins to clients all over the world. Client satisfaction is our relentless pursuit. If you want to know more about this, please feel free to contact us.
Reference
- Ma, M.F.; et al. Polysaccharide from edible alga Enteromorpha clathrata improves ulcerative colitis in association with increased abundance of Parabacteroides spp. in the gut microbiota of dextran sulfate sodium-fed mice. Mar. Drugs. 2022, 20(12): 764.
