Antimicrobial Activity Screening Service at CD BioGlyco
CD BioGlyco is a glycobiology company with a team of specialized researchers dedicated to the study of various glycans. Since antimicrobial activity is one of the most important characteristics of marine glycans and glycoproteins, CD BioGlyco offers a wide range of assays to analyze the antimicrobial activity of marine glycans and glycoproteins.
We provide an Oxford cup method to test the antimicrobial activity of marine glycans and glycoproteins.
We provide to explore the mechanisms of antimicrobial action of marine glycans and glycoproteins, for example:
- Inhibits bacterial cell adhesion
- Damage or increased permeability of bacterial cell membranes and cell walls
- Inhibit bacterial nucleic acid and protein synthesis
- Influence bacterial metabolism
- Inhibit biofilm formation or damage biofilm
Further, we provide antimicrobial activity screening methods that include, but are not limited to:
Fig.1 Antimicrobial activity screening services. (CD BioGlyco)
Diffusion Method Assay
We place the glycans and glycoproteins to be tested in specific vessels and use their diffusion to inhibit the growth of bacteria. Ultimately, the antibacterial activity of the glycans and glycoproteins is determined based on the size of the circle of inhibition. CD BioGlyco offers clients specific methods such as the agar disk-diffusion method, the antimicrobial gradient method, and other methods.
Thin-layer Chromatography (TLC)-bioautography Assay
The method is a combination of TLC separation and biological activity determination. CD BioGlyco offers agar diffusion assays, direct bioautography assays, and agar overlay bioassays for testing.
Dilution Method Assay
CD BioGlyco makes dilutions of the glycans and glycoproteins to be tested at different concentrations, and after inoculation of the strain to be tested, quantifies the lowest inhibitory concentration of the glycans and glycoproteins to be tested that inhibits or kills the bacteria to be tested. CD BioGlyco offers a variety of specific dilution methods for analyzing the antimicrobial activity of the test substance to be tested, such as the broth dilution method, agar dilution method, and more.
Time-kill Assay
The specific procedure of the time-kill assay service provided by CD BioGlyco is as follows: a certain concentration of marine glycans and glycoproteins are added to the test bacterial solution for co-incubation, and the colony-forming units are counted at different time points, and the logarithmic value of the time-colony count curve is plotted. The curve can be used to compare the sterilization speed and duration of different substances to be tested or different concentrations of the same substance.
ATP Bioluminescence Assay
When an organism dies, ATP is broken down by the action of enzymes. CD BioGlyco uses this as the basis for determining the ATP concentration in the sample, which can be used to derive the number of viable organisms. The ATP bioluminescence assay involves the following steps: sampling, extraction, addition of fluorescein, measurement of bioluminescence, and derivation of the ATP concentration and the number of viable organisms.
Flow Cytofluorometric Method
The basic principle of flow cytometry is that as long as the desired test particle can be labeled with a fluorescent dye it can be quantified. During the detection of bacteria, enhanced fluorescent signals are observed in bacterial cells with active metabolic processes. CD BioGlyco will use this as the basis for providing clients with a flow cytometric fluorescence assay to detect the antimicrobial activity of marine glycans and glycoproteins.
Publication
Technology: Fluorescent labeling technology and in vitro experiments
Journal: Applied Biochemistry and Biotechnology
IF: 3
Published: 2023
Results: The authors generated crude fucoidan by employing microwave and probe sonication and purified it using DEAE-cellulose anion exchange chromatography. Structural analysis showed the presence of sulfate and hydroxyl groups in the isolated fucoidan. According to nuclear magnetic resonance (NMR) data, fucoidan residues were present. Next, the research investigated the biological activity of fucoidan and found it to be an effective weapon against Proteus vulgaris and Salmonella enterica. Fluorescence microscopy showed that the anti-biofilm effect of fucoidan was fully effective against biofilm formation as well as planktonic cell growth of Aspergillus common as well as Salmonella at doses exceeding 25 g/mL. Then, through in vitro research on both bacterial species, the authors explored the anti-ionic sensing and anti-biofilm capabilities of fucoidan. Finally, fucoidan was found to be useful for the production of antimicrobial compounds and to act as a population-sensing inhibitor against side effects and antibiotic resistance.
Fig.2 Inhibitory effect of fucoidan on two bacteria by fluorescence microscopy. (Mani, et al., 2023)
Applications
- Marine glycan antimicrobial activity screening services can be used in the development of antimicrobial compounds and bacterial inhibitors.
- Marine glycan antimicrobial activity screening services can be used in research on bacteria-induced diseases.
- Marine glycan antimicrobial activity screening services could be used for the development of marine glycan-based antimicrobial vaccines.
Advantages of Us
- We have a wide range of methods for Bioactivity Analysis to choose from to ensure that our client's research needs are met.
- Our research team has a background in marine glycan bioactivity research to customize analysis solutions for our clients.
- We have the world's leading antimicrobial activity analysis technology to provide our clients with high-quality bioactivity testing solutions.
CD BioGlyco offers marine glycans and glycoproteins antimicrobial activity analysis service, we have a high-level laboratory and every assay protocol is tested for quality. If you are interested in our services, please feel free to contact us.
Reference
- Mani, G.; et al. Evaluation of antibiofilm and antiquorum sensing activities of fucoidan characterized from Padina boryana against Nosocomial Pathogens. Applied Biochemistry and Biotechnology. 2023.
