Efficient and Customized Released Marine Glycan Analysis Service Rely on Professional Team
Glycans have certain biological activities, such as antiviral, and also affect the structure and functions of glycoproteins. Their analysis is crucial in drug discovery, glycosylation modification research, etc. Based on a well-established and efficient analytical process, CD BioGlyco provides a professionally released marine glycan analysis service. We understand each client's needs and provide customized analytical solutions to help fast-track their research.
Released Marine Glycan Analysis Service at CD BioGlyco
The glycan analysis process includes glycan release, purification, labeling/derivatization, and analysis. Our released marine glycan analysis process has been optimized for good reproducibility and accurate and reliable results. The process is described below:
- Sample pre-treatment: We perform a preliminary treatment of the sample to remove impurities and extract it using an appropriate solvent. High-purity samples containing the target glycans are obtained through purification, concentration, etc.
- Release of glycans: Glycans are released from glycoproteins or glycolipids using chemical or enzymatic methods.
- Purification: The released glycan is purified by techniques such as chromatography to remove impurities, other non-volatile salts that interfere with the derivatization reaction, substances with free amino groups, etc.
- Glycan derivatization: We fluorescently label or chemically derivatize glycans to improve the sensitivity of glycan detection. Excess-free labeling is removed to ensure accurate results.
- Analysis: We use advanced techniques to analyze the monosaccharide composition, structure, glycosidic bond type of glycans, etc. Liquid chromatography-mass spectrometry (LC-MS), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), etc., are efficient techniques for glycan analysis. We also have efficient bioinformatics tools to analyze the data in depth.
Glycans affect the structure, stability, interaction with other molecules, and functions of glycoproteins. In addition to glycans, we offer a specialized Marine Glycopeptide Analysis Service.
Applicable Samples
Released marine glycan analysis is suitable for a wide range of marine samples, including algae, fish, and other marine organisms. Clients only need to provide experimental objectives and samples. Glycan release, purification, and analysis are all done by us.
Delivery of Results
The cycle time of the released marine glycan analysis project varies depending on the type of sample and the complexity of the experimental procedure. After analysis, we deliver a detailed analysis report including the following:
- Experimental methods and analytical procedures
- Analytical parameters
- Raw data
- Mass spectra
- Released marine glycan analysis results
Publication Data
Technology: Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ES-MS/MS)
Journal: International Journal of Molecular Sciences
IF: 4.9
Published: 2023
Results: In this study, the composition and structure of shark skin mucus were investigated by glycan analysis and histological analysis. After releasing glycans from three animals (tiny dogfish, chain catshark, and little skate), the researchers performed glycan profiling by LC-ES-MS/MS and characterized the N-glycans and O-glycans of these three animals. Eventually, 22 and 39 glycans were identified from little skates and tiny dogfish, respectively, and the fucosylation modifications on the glycans were abundant. The glycans were predominantly O-glycans in dogfish and were very similar to human gastrin O-glycans. Three glycans with Galβ1-3/4GlcNAc repeats were identified in chain catshark. This study provides data to support the study of the functional role and potential biomedical significance of shark skin mucus glycans, as well as a model for the analysis of glycans in other marine organisms.
Fig.1 Glycans detected in spiny dogfish. (Bachar-Wikstrom, et al., 2023)
Applications
- Glycans contained in marine organisms have certain antiviral activities. Released marine glycan analysis plays an important role in the development of antiviral drugs.
- Released marine glycan analysis plays an important role in studying the functions and potential biological significance of glycans.
- Released marine glycan analysis is used to identify substances that are used in biomaterials, drug delivery systems, and more.
Advantages
- We customize our solutions for specific sample types or research objectives to fully meet our clients' research needs.
- We utilize advanced technology and efficient testing processes to analyze released marine glycan.
- We provide detailed data interpretation and comprehensive analysis reports to help dig deeper into the glycan analysis data.
Frequently Asked Questions
What are the biological functions of marine glycans?
Marine glycans have important regulatory functions and good biological activities, including antiviral activity, and antibacterial properties. In addition, glycans are involved in forming glycoproteins and glycopeptides, immune regulation, cell signaling, and providing viscosity and adhesion.
What are the techniques used for N-Glycan analysis?
- MALDI-TOF MS: This technique enables high-throughput analysis of N-glycans with high sensitivity. Detection is possible after the release and methylation of N-glycans by an enzymatic reaction.
- LC-MS: It is a commonly used method for N-glycan analysis. After releasing N-glycans by enzymatic reaction, fluorescent labeling is used to analyze many samples. Care should be taken to remove detergents and other impurities that interfere with the derivatization reaction before fluorescent labeling.
At CD BioGlyco, we provide an innovative released marine glycan analysis service to provide strong support for our client's research. Welcome to contact us if you have any questions. We look forward to exploring the structure, biological functions, ecological roles, and applications of marine glycans with you.
Reference
- Bachar-Wikstrom, E.; et al. Identification of novel glycans in the mucus layer of shark and skate skin. International Journal of Molecular Sciences. 2023, 24(18): 14331.
