Pure Nature, Empowering the Frondoside A Production Journey
Relying on highly specialized technology and strict quality control, CD BioGlyco provides high-quality Marine Biomolecule Production services. Frondoside A is a triterpene glycoside derived from sea cucumber with a wide range of biological activities. Its molecular weight is 1334 Da. Our experienced scientists provide high-quality frondoside A production services including extraction, purification, characterization, and activity determination. Our skilled researchers provide frondoside A with specific purity and specification according to the client's needs.
Extraction and Purification of Frondoside A
The collected sea cucumbers are cleaned, cut into pieces, and frozen for storage.
- Ethanol extraction: Small pieces of biological samples are extracted 2-3 times using hot ethanol. Our researchers do consolidation and low-pressure concentration processes of the extracts.
- Chromatography purification: Our researchers use macroporous adsorbent resin chromatography to separate the crude extracts. We use different eluents to separate the individual components. For example, we elute inorganic salts and polar impurities with water and target fractions with ethanol. Our operators have many years of operational experience in glycoside purification. For the eluted mixed fractions, we offer purification services by silica gel chromatography and high-performance liquid chromatography (HPLC).
Characterization of Frondoside A
CD BioGlyco has a dedicated analytical team to provide accurate structural identification services by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.
Activity Assay of Frondoside A
- Cancer cell proliferation assay
- Cell viability assay
- Programmed cell death assay
- Cell apoptosis assay
- Quantitative real-time polymerase chain reaction analysis
- Cancer growth assay
- Cell migration assay
- Anti-angiogenic activity assay
- Cell cycle assay
- Cell morphology analysis
Publication
Technology: HPLC, Cell viability assay, Cell migration assay, Olympus inverted microscope, Cell cycle analysis
Journal: Nutrients
Published: 2023
IF: 4.546
Results: In this work, researchers tested and evaluated frondoside A derived from sea cucumber, as a potential anti-bladder cancer drug. It was shown that frondoside A displayed significant anti-tumor activity, affected cell cycle distribution, and induced apoptosis in a concentration-dependent manner. Moreover, the researchers analyzed the tumor growth effects in nude mice. In vivo results showed that frondoside A significantly reduced the growth of tumor xenografts and no significant toxic side effects were observed. This literature provides important information for a deeper understanding of the potential of marine natural products in cancer therapy.
Fig.1 Schematic diagram of cell viability and migration capacity test. (Ru, et al., 2023)
Applications
- Marine drug development: Frondoside A plays an inhibitory role in the proliferation and migration of malignant tumor cells or even directs apoptosis. Therefore it is a good material for the development of anticancer drugs.
- Inhibition of amyloid aggregation: Frondoside A prevents Amyloid-β (Aβ)-induced toxicity by inhibiting the formation of Aβ oligomers. It provides valuable value in Alzheimer's disease research.
- Immunomodulation: Frondoside A can be used to develop immunostimulants for cellular immunity.
Advantages of Us
- CD BioGlyco provides clients with comprehensive technical support and consulting services to help them solve all kinds of problems encountered in the process of using frondoside A to ensure its application effect.
- Our technicians follow the whole production process and report the progress to clients on time.
- All analyzed monitoring data is recorded and shared to your email on time.
- As a specialized marine molecule manufacturing company we offer many types of frondoside such as frondoside A-F.
Frequently Asked Questions
What are the potent bioactive effects of frondoside A?
Frondoside A has inhibited cancer cell proliferation, migration, invasion, angiogenesis, induced apoptosis, and immunomodulatory effects.
What are the structural features of Frondoside A?
Frondoside A has an acetyl group at glycoside C-16. It contains xylose and 3-O-methylglucose as the third and fourth monosaccharide residues, respectively. Uniquely, it has a sulfate group on the first sugar residue.
CD BioGlyco provides efficient frondoside A production services with specialized production staff. No matter which type of frondoside you want, our laboratory offers you the perfect production solution. Contact us at your convenience for assistance.
Reference
- Ru, R.; et al. Sea cucumber derived triterpenoid glycoside frondoside A: a potential anti-bladder cancer drug. Nutrients. 2023, 15(2): 378.
