Bioactivity Analysis of Marine Glycan&Glycoprotein

Bioactivity Analysis of Marine Glycan&Glycoprotein at CD BioGlyco

Marine glycans and glycoproteins are substances of marine origin with a variety of physiological activities, which are now widely used in many fields. CD BioGlyco has a professional laboratory that provides a variety of services related to marine glycans and glycoproteins to clients from all over the world.

• First of all, we provide purification services for marine glycans and glycoproteins by graded precipitation, quaternary ammonium precipitation, membrane separation, gel column chromatography, and so on.
• Second, we provide a variety of methods to analyze the biological activity of marine glycans and glycoproteins. For example:
  • To construct a mice model and a blank control group to analyze the antidiabetic as well as antitumor activity.
  • Provides cellular activity assays and animal activity assays to test marine glycans and glycoproteins for their antioxidant, hypoglycemic, antimicrobial, and other activities.

Fig.1 Bioactivity assay of marine glycans and glycoproteins. (CD BioGlyco)

Among them, the bioactivity analysis services include but are not limited to the following:

Antioxidant Activity

Various free radicals and oxygen damage organisms and are an important cause of aging and death. CD BioGlyco offers a variety of antioxidant activity screening services for different oxidizing substances. The methods we offer include but are not limited to 1,1-Diphenyl-2-picrylhydrazyl, Hydroxyl Radical, Superoxide Radica Scavenging, Ferrous Ion Chelating Ability, Lipid Peroxidation Inhibition assay.

Antimicrobial Activity

The antimicrobial activity of marine glycans and glycoproteins has been shown to have a favorable inhibitory effect on diseases caused by certain strains of bacteria. We offer screening methods for antimicrobial activity:

• Diffusion Methods

In addition, we offer other diffusion methods to expand the field of Antimicrobial Activity assay, such as agar plug diffusion, agar well diffusion, cross streak, poisoned food method, and so on.

• Thin Layer Chromatography-BioAutomated

• Dilution Methods

• Time-Based Sterilization
• ATP Bioluminescence
• Flow Cytofluorescence

Antiviral Activity

Viruses are an important cause of disease and death in organisms. CD BioGlyco offers a variety of antiviral activity screening services based on the infectivity of the virus. In addition, we offer quantitative testing for viruses.

• Infectivity Assays

• Virus Quantitation Assays

We also provide enzyme-linked immunosorbent, fluorescent-tagged antibody, and precipitation assay during serum testing to ensure high efficiency in helping our clients complete virus quantification.

Anti-blood Clotting Activity

Marine glycans exhibit significant anti-blood clotting activity. CD BioGlyco offers assays to screen for anti-blood-clotting activity based on the principles of anti-blood-clotting. The assay services we provide include but are not limited to Partial Thromboplastin Time Assay, Prothrombin Time Assay, Activated Partial Thromboplastin Time Assay, Activated Coagulation Time Assay, and Thrombin Clot Time Assay.

Antitumor Activity Screening

The significant immune-enhancing activity of marine glycans provides ideas for research on their anti-tumor mechanism of action. CD BioGlyco provides Reactive Oxygen Species and Cellular Assays based on tumorigenesis principles and processes. In the cellular assay process, we have a variety of detection pathways, such as MTT proliferation assay, Rhodamine 123 accumulation assay, Apoptosis detection, and Proliferation inhibition-mitotic index assay. Naturally, we offer customized anti-tumor assays according to our clients' needs.

Antiinflammatory Activity

Marine organisms create a huge library of compounds for screening anti-inflammatory drugs. CD BioGlyco provides clients with screening services in Hemolysis Inhibition, Proteinase Inhibition, Protein Denaturation Inhibition, and Lipoxygenase Inhibition based on the cause and manifestation of inflammation.

Probiotic Activity

Marine glycans are an important source for the development of innovative probiotics. Additionally, probiotics have sensitivities to pH, bile salt, and antibiotics. CD BioGlyco uses this as a basis for providing Low pH Tolerance Assay, Bile Salt Tolerance Assay, and Antibiotic Susceptibility Assay as well as other testing services for marine glycans.

Hepatoprotective Activity

Enzymes are a key factor in liver activity. CD BioGlyco offers testing for Alanine Aminotransferase, Aspartate Aminotransferase, and Alkaline Phosphatase, and further offers targeted Liver Function Measurements.

Antidiabetic Activity

Marine glycans exhibit antidiabetic activity with hypoglycemic and glucosidase inhibitory activities. The services offered by CD BioGlyco to test for anti-diabetes are Glucose Metabolism, Cellular Glucose Utilization, Cellular Triglyceride Accumulation, Rat Pancreatic Insulinoma Proliferation, α-Amylase Inhibition, α-Glucosidase Inhibition, Pancreatic Lipase Inhibition, Dipeptidyl Peptidase IV Inhibition assays, and so on.

Publication Data

Technology: Microwave-assisted extraction, ultrasound-assisted extraction, enzyme-assisted extraction, pressurized liquid extraction, and pulse-electric field extraction method

Journal: Food Hydrocolloids

IF: 10.7

Published: 2023

Results: A sulfated polysaccharide mainly from brown seaweeds, consisting of a pyranofucose backbone and several monosaccharides, fucoidan possesses a variety of biological activities such as anticancer, antioxidant, immunomodulatory, antiviral, antithrombotic, and anti-inflammatory properties. In this thesis, the authors describe information about fucoidan green extraction methods, purification, and structural analysis. They also discuss the factors affecting the bioactivity of fucoidan. The structure complexity of fucoidan depends on factors such as species, source, and harvest time. In turn, this greatly affects the bioactivity of fucoidan, with high sulfation and low molecular weight being particularly associated with increased bioactivity. The presence of a sulfated fraction makes fucoidan easy to interact with other polymers and has far-reaching applications.

Applications

• Marine glycans and glycoproteins bioactivity analysis service can be used in the development of blood coagulants and anti-tumor, anti-diabetic, and anti-inflammatory drugs.
• Marine glycans have a variety of biological activities, which are closely related to the structural characteristics of the glycans such as degree of polymerization, monosaccharide composition, and sequence, and the number and position of modifying groups. Therefore, the bioactivity analysis of marine glycans can be used for the development of glycans with special properties.
• The bioactivity analysis of marine glycans can be used for the research of active polysaccharide components in various marine organisms.

Advantages of Us

• Our service team has a deep background in marine glycans and glycoproteins research and is proficient in a wide range of analysis techniques for marine glycans to support all of our client's requirements.
• We offer multiple methods for each bioactivity analysis of marine glycans and glycoproteins.
• We provide not only bioactivity assays but also analysis of all assay data.

CD BioGlyco has a passionate research and development team with rich experience in basic research on marine active glycans as well as full-process glycan production. We pursue technological leadership, embrace global wisdom, and operate with integrity to bring product value and application value to our clients. If you want to know more, please feel free to contact us today.