Your Gateway to Marine Polysaccharide Insights-Comprehensive Capsular Polysaccharide (CPS) Characterization Service
At CD BioGlyco, we are deeply rooted in the forefront of biotechnology and are committed to providing accurate and comprehensive Marine Carbohydrate Characterization Services to clients around the world. As the cornerstone of this comprehensive service system, we provide Marine Microbial Polysaccharide Characterization Services, which aim to reveal the complex structure and biological activity of polysaccharides from marine microorganisms.
CPS characterization service is one of our core expertise. We use advanced molecular structure analysis technology, purity detection methods, glycan chain composition analysis, functional group identification, and a full range of strategies for physicochemical properties and biological activity evaluation to provide clients with detailed characterization reports on marine microbial CPSs. These services lay a solid foundation for subsequent scientific research, product development, and market application.
Molecular Structure Analysis
- 1H nuclear magnetic resonance (NMR) and 13C NMR: These techniques provide detailed information on the hydrogen and carbon environments within the polysaccharide.
- 2D NMR (COSY, HSQC, HMBC): We employ these methods to establish connectivity and proximity relationships among nuclear spins, allowing us to decipher the polysaccharide's complex molecular structure.
- MALDI-TOF MS: Used for determining the molecular weight distribution and structure of the polysaccharide.
- ESI-MS/MS: Allows for the elucidation of fine structural details, including sequence, branching, and linkage information.
Purity Detection
- Size exclusion chromatography (SEC): This technique is utilized to assess the distribution of molecular weights and establish the purity of the polysaccharide.
- Capillary electrophoresis (CE): Provides high-resolution analysis and purity assessment of the polysaccharides.
Glycan Chain Composition Analysis
- Gas chromatography-mass spectrometry (GC-MS): Used after derivatization (e.g., methylation analysis) to identify and quantify the monosaccharide composition of the polysaccharide.
- Liquid chromatography-mass spectrometry (LC-MS): Allows for the detailed profiling of oligosaccharides resulting from enzymatic digestion of the polysaccharide.
Functional Group Identification
- Fourier transform-infrared (FTIR) spectroscopy: We utilize this method to detect and identify functional groups within the polysaccharides by their characteristic absorption bands.
- Raman spectroscopy: This method supplements IR spectroscopy by offering extra insights into the vibrational modes that are unique to the molecular structure.
Physicochemical Property Determination
- Solubility: We perform solubility tests in various solvents to determine the solvent compatibility of the polysaccharide.
- Rheometry: Used to measure the flow and deformation behavior of the polysaccharide solutions under different conditions.
- Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC): Provide insights into the thermal stability and transitions (e.g., melting point, glass transition) of the polysaccharides.
Biological Activity Evaluation
- Immunomodulation: For In vitro assays, we use cell lines to measure cytokine production, cell proliferation, and other immune responses upon exposure to the polysaccharide. For In vivo studies, researchers can use animal models to gain a deeper understanding of the immunomodulatory effects.
- Antioxidant capacity testing: DPPH assay measures the ability of the polysaccharide to scavenge free radicals. ABTS assay is another method to assess the antioxidant potential by evaluating the reduction of the ABTS radical cation.
Publication
Technology: Structure and bioactivity of the CPS from Psychrobacter marincola
Journal: Marine Drugs
IF: 6.085
Published: 2020
Results: The authors reported on the structure of the CPS from Psychrobacter marincola KMM 277T and its impact on the viability and colony formation of human acute promyelocytic leukemia HL-60 cells. They purified the polymer using various separation methods, such as ultracentrifugation and chromatographic techniques. The structure was determined through chemical analysis and 1-D and 2-D NMR spectroscopy. The biological analysis revealed that the CPS significantly diminished the viability and colony formation of HL-60 cells.
Fig.1 HPSEC elution profile and alcian blue-stained electrophoregram of the CPS from P. marincola KMM 277T. (Kokoulin, et al., 2020)
Applications of Marine CPS
- CPSs have the potential to enhance the texture, stability, and flavor of food products.
- Taking advantage of its unique molecular structure and physicochemical properties, marine CPSs can be used as templates or stabilizers to participate in the preparation process of nanomaterials.
- Due to its unique biocompatibility and degradability, marine CPSs can be used to modify biomaterials such as tissue engineering scaffolds, drug carriers, and medical dressings to enhance the stability and functionality of the materials.
Advantages
- We use modern analytical techniques to accurately analyze the molecular structure of marine CPSs, including glycan chain composition, connection mode, and conformational characteristics.
- We establish a comprehensive functional evaluation system to systematically evaluate the potential functions of marine CPSs in immunomodulation, antibacterial and antiviral, and antioxidant through cell experiments and molecular simulations.
- We implement a strict quality control process from raw material collection to product output. Each step is carefully designed and strictly monitored to ensure the stability and reliability of the characterization results of marine CPSs.
Frequently Asked Questions
What is marine CPS and why is its characterization so important?
Marine CPS is a class of complex carbohydrates derived from the outer layer of marine microbial cells. They have unique biological activity and chemical structure and show great application potential in many fields such as biomedicine, functional food, and cosmetics. Characterizing the structure, composition, physicochemical properties, and biological functions of these polysaccharides is essential for understanding their working mechanism, optimizing extraction processes, and developing new products. Through professional characterization services, clients can gain an in-depth understanding of the characteristics and potential of marine CPSs, providing a scientific basis for scientific research and product development.
What specific characterization services does your company provide?
Our marine CPS characterization services include but are not limited to molecular structure analysis (such as NMR and MS analysis), purity detection, glycan chain composition analysis, functional group identification, physicochemical property determination (such as solubility and viscosity), and biological activity evaluation (such as immunomodulation and antioxidant capacity). We tailor characterization solutions according to the specific needs of clients to ensure the comprehensiveness and professionalism of our services.
At CD BioGlyco, we use a range of advanced methods to provide clients with in-depth and accurate marine CPS characterization services. Please feel free to contact us for more details if you are interested in our marine CPS characterization service!
Reference
- Kokoulin, M.S.; et al. Structure and in vitro bioactivity against cancer cells of the capsular polysaccharide from the marine bacterium Psychrobacter marincola. Marine Drugs. 2020, 18(5): 268.
