Technologies for Marine Carbohydrate Characterization

Technologies for Marine Carbohydrate Characterization at CD BioGlyco

Bioactive Compounds derived from the sea are readily available, nontoxic, cheap, biodegradable, and biocompatible, finding interesting and valuable applications in the food and medicine field. In the pharmaceutical field, chitin and chitosan extracted from animal shells are used in wound dressings, hypolipidemic agents, blood anticoagulants, antithrombotic agents, and Drug Delivery Systems. Laminarin, alginate, and fucoidan extracted from brown algae cell walls have a high potential for biological applications in functional foods and cosmetics. To help clients fully explore and study marine carbohydrates, CD BioGlyco has developed a one-stop Marine Carbohydrate Production and Characterization solution. In addition, we also provide our clients with world-class marine carbohydrate application development services, such as Biomarker Development and Mimetic Development. Our services are as follows:

Extraction and Purification of Marine Carbohydrate

We separate different carbohydrates from large marine algae, marine animals, and marine microorganisms through various extraction techniques and go through purification processes to obtain different fractions.

Characterization of Marine Carbohydrates

Generally, the chemical structure of compounds determines their physical and chemical properties as well as biological activities. To better analyze the physical, chemical, and biochemical properties and biological activities of different fractions of carbohydrates, we use a variety of technologies to help clients analyze and resolve the structures of compounds and develop their applications in the medical field.

• Marine carbohydrate characterization by high-performance liquid chromatography (HPLC): HPLC is the most commonly used chromatography technique for separating different fractions of compounds and determining their purity.
• Marine carbohydrate characterization by Fourier-transform infrared spectroscopy (FTIR): FTIR is used to identify and characterize compounds by creating spectra that show molecular vibrations.
• Marine carbohydrate characterization by nuclear magnetic resonance (NMR): NMR allows people to obtain more information about the molecular structure and analyze the structure of compounds.
• Marine carbohydrate characterization by gas chromatography (GC): GC is used to separate volatile compounds and has the advantages of high sensitivity, fast analysis, good accuracy, and small sample volume.
• Marine carbohydrate characterization by mass spectrometry (GC-MS): GC-MS is used for the analysis and quantification of organic volatile and semi-volatile compounds.
• Marine carbohydrate characterization by atomic force microscopy (AFM): AFM is used to study the surface structure and properties of substances.
• Marine carbohydrate characterization by scanning electron microscopy (SEM): SEM is used for elemental trace analysis and particle characterization.
• Marine carbohydrate characterization by X-ray diffraction (XRD): XRD is used to characterize crystalline materials, providing information such as average grain size, crystallinity, strain, and crystal defects.
• Marine carbohydrate characterization by energy dispersive X-ray spectroscopy (EDS): EDS is used to determine elemental composition, and identify and map elements present in defects.
• Marine carbohydrate characterization by gel permeation chromatography (GPC): GPC is used to separate compounds in organic solvents based on their molecular size.
• Marine carbohydrate characterization by differential scanning calorimetry (DSC): DSC is used to study the relationship between the heating rate of compounds and temperature.
• Marine carbohydrate characterization by thermogravimetric analyzer (TGA): TGA is used to study the temperature-mass change relationship of compounds.
• Marine carbohydrate characterization by Fourier transform Raman (FT-Raman): It has the advantages of high resolution, high sensitivity, full automation, and high reliability.
• Marine carbohydrate characterization by carbon hydrogen nitrogen sulfur (CHNS) elemental analyzer: CHNS elemental analyzer is used to quantitatively analyze carbon, hydrogen, nitrogen, and sulfur elements simply, accurately, and efficiently.
• Marine carbohydrate characterization by frontal affinity chromatography technology (FACT): FACT is used to evaluate the glycan-binding profiles of lectins.
• Marine carbohydrate characterization by fluorescein isothiocyanate (FITC)-labeled: FITC labels compound molecules for better imaging analysis.

Fig.1 Technologies for marine carbohydrate characterization. (CD BioGlyco)

Publication

Technology: FTIR, NMR

Journal: Advances in Bioscience and Biotechnology

IF: 1.18

Published: 2020

Results: The authors extracted sulfated heteropolysaccharides from Ulva species collected in the natural environment (SEA) and cultured in nutrient-rich water (CULT), and performed structural analysis and characterization by FTIR and NMR techniques. The results showed that ulvanobiose was present in ulvan extracted from SEA, while most of the A-type disaccharide signals were present in ulvan extracted from CULT.

Fig.2 A) Infrared spectra; B) ¹³C NMR spectra; C) ¹H NMR spectra. (Figueira, et al., 2020)

Highlights

• We use a variety of analytical techniques to help you characterize the structure of the compounds quickly, accurately, and efficiently.
• We combine multiple technologies at the same time to analyze the structural fragment information of unknown compounds and solve the structure of the compound.
• With a variety of technical means, we perform element trace analysis, crystal structure analysis, temperature-mass change relationship analysis, etc.

Frequently Asked Questions

Why characterize the structure of marine carbohydrates?

Marine organisms are a treasure trove of natural medicines, and most marine organisms produce some sticky substances or polysaccharide polymers. They have various physiological regulatory functions, such as lowering blood sugar, lowering blood lipids, scavenging free radicals, anticoagulation, antithrombosis, anti-arrhythmia, antitumor, etc. Understanding the structure of marine polysaccharides helps elucidate its structure-activity relationship and biological activity mechanism, discover new biological functions, and modify its structure.

How to ensure the accuracy of polysaccharide analysis structure?

1) Correctly understand polysaccharide structure types: Many researchers believe that polysaccharide structure analysis is characterized by uncertainty, low reproducibility, and irregularity. We are good at using existing knowledge to scientifically analyze and judge polysaccharides containing multiple monosaccharides and glycosidic bond types to obtain better analytical data; 2) Improve polysaccharide extraction and preparation efficiency and ensure sample reproducibility; 3) Use polysaccharide structure characterization methods based on "partial degradation-methylation-NMR" as the core to promote polysaccharide structure research.

With years of analytical experience and expertise, CD BioGlyco provides you with the fastest, most accurate, and most sensitive characterization services for marine carbohydrates. If you have any characterization needs, please feel free to contact us for information and a quote.