Sargassum pallidum Polysaccharide Adjuvant Development Service

Sargassum pallidum Polysaccharide Adjuvant Development Service at CD BioGlyco

CD BioGlyco offers comprehensive solutions related to marine glycan that cover Marine Biomolecule Production Services, Marine Carbohydrate-based Vaccines Development Services, and Marine Carbohydrate-based Adjuvant Development Services. Our team of skilled scientists is highly proficient in delivering customized services in the field of Sargassum pallidum polysaccharide adjuvant development.

Solution Adjuvant Development

We dissolve Sargassum pallidum polysaccharides in a suitable solvent, such as water or saline, to form a stable solution. Its biological activities including antioxidant, anti-inflammatory, and immunomodulatory, are evaluated by in vitro and in vivo experiments.

Gel Adjuvant Development

We select appropriate gelling or cross-linking agents to convert Sargassum pallidum polysaccharides into a gel state. The gelators we usually use include gelatin and sodium alginate, and the commonly used cross-linking agents include aluminum sulfate and ethylene glycol dimethylpropane. Subsequently, we evaluate the temperature stability, drug compatibility, and mechanical stability, and then they are characterized by using scanning electron microscopy and transmission electron microscopy. We then regulate the rate and mode of drug release to meet the drug delivery requirements. Finally, we evaluate the biocompatibility of the products, which includes cytotoxicity tests, in vivo and in vitro tests in animals.

Fig.1 Process of Sargassum pallidum polysaccharide adjuvant development. (CD BioGlyco)

Publication Data

Technology: Polysaccharide extraction and immunological efficacy assessment techniques

Journal: Marine Drugs

IF: 5.4

Published: 2012

Results: This paper investigated the effect of Sargassum pallidum polysaccharide as an adjuvant on the immune activity of three poultry vaccines. The authors added Sargassum pallidum polysaccharide to three vaccines, namely Newcastle disease, infectious bronchitis, and avian influenza, and evaluated the adjuvant effect of the polysaccharide by measuring the immunity indexes of the poultry after vaccination. The results showed that the addition of Sargassum pallidum polysaccharide significantly improved the immunization effect of the three vaccines. In the Newcastle disease vaccine, the polysaccharide group showed a 1.5-fold increase in antibody titer compared with the control group. In the infectious bronchitis vaccine and avian influenza vaccine, the polysaccharide group showed an enhancement in cellular immune response and antibody titer compared with the control group. The authors also evaluated the in vivo toxicity of the polysaccharides and showed that Sargassum pallidum polysaccharides had no significant toxic side effects in poultry. Therefore, the study suggests that Sargassum pallidum polysaccharide is effective in poultry vaccine research and has a promising application.

Applications

• In the field of health food, Sargassum pallidum polysaccharides are used as adjuvants to enhance immunity, improve gastrointestinal function, supplement trace elements, and delay aging.
• Sargassum pallidum polysaccharides can be used as carriers or release systems for drugs to improve stability, bioavailability, and targeting.
• As a natural polymer material with good biocompatibility and biodegradability, they are widely used in the preparation of biodegradable packaging materials.

Fig.2 Anticancer activity of seaweed polysaccharide. (Nigam, et al., 2022)

Highlights

• Sargassum pallidum polysaccharides are generally well-biocompatible which makes the adjuvants more reliable and safe for drug and vaccine development.
• Sargassum pallidum polysaccharides can be applied in different types of vaccines and pharmaceutical adjuvants.
• We have established a wide range of relationships with partners in various fields and are able to work together collaboratively to advance the development and application of Sargassum pallidum polysaccharide adjuvant.

Frequently Asked Question

• Q1: How to characterize the structure of Sargassum pallidum polysaccharides?
  We determine the structure and connectivity of polysaccharides by analyzing the chemical shifts and couplings of atoms such as hydrogen, carbon, and nitrogen in a sample by nuclear magnetic resonance. Information such as the molecular mass and fragment ions of polysaccharides can be analyzed by measuring the mass-to-charge ratio of molecular ions in the sample.
• Q2: How to assess the biocompatibility of Sargassum pallidum polysaccharides?
  We evaluate the toxic effects of sargassum polysaccharides on cells by in vitro cultured cell lines, such as human fibroblasts and macrophages. The biocompatibility and biological effects of Sargassum pallidum polysaccharides in vivo are studied by animal models, such as subcutaneous implantation tests and injection tests. Hematocompatibility testblood compatibility tests.

The application of Sargassum pallidum polysaccharide as an adjuvant in biopharmaceutical and glycomedical research requires in-depth studies of its structure and function. CD BioGlyco is well-positioned to provide expertise and services in the development of Sargassum pallidum polysaccharide adjuvant. Please contact us if you have any questions regarding our services.