Hyaluronic Acid (HA)-based Tissue Gel Development Service at CD BioGlyco
CD BioGlyco specializes in the development of hydrogel Biomaterials and the preparation of related products. Hydrogels are three-dimensional network structures formed by hydrophilic polymers through physical or covalent cross-linking. HA is an important component of cellular matrices and many tissues and has a variety of important physiological functions. Based on its unique rheological properties, hygroscopicity, degradability, biocompatibility, ease of modification, etc., it has a wide range of applications in tissue engineering. We provide an HA-based tissue gel development service. Our goal is to meet the needs of our clients for different applications in the biomedical field.
Fig.1 Reasons for choosing HA to develop biomaterials. (CD BioGlyco)
Design and Preparation of HA-based Hydrogels
We design HA-based hydrogel for its chemical structure, composition, biofunctional degradability, rheological properties, resistance to enzymatic degradation, mechanical integrity, etc. HA-based hydrogels are formed by a variety of methods. We have a variety of cross-linking methods to choose from, including click chemistry (Diels-Alder reaction, azide-alkyne Huisgen cycloaddition, etc.), enzymatic cross-linking, disulfide cross-linking, free radical polymerization cross-linking, etc. Development directions of HA-based hydrogel include drug delivery, targeted cancer therapy, and tissue engineering studies of skin, vocal cords, and cartilage.
After preparation, we Characterize the structure and composition of the products. The degree of derivatization is determined by spectral characterization such as nuclear magnetic resonance and Fourier transform infrared spectroscopy. Mechanical properties are evaluated including microstructural characterization, rheology, water retention, etc. Biocompatibility is also tested.
Fig.2 Development process of HA-based hydrogel. (CD BioGlyco)
Chemical Modification of HA
HA is a naturally occurring macromolecular Glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine. It contains hydroxyl, carboxyl, and amide groups that are chemically modified. The derivatives obtained after modification retain the biodegradability and biocompatibility of natural HA. Biomaterials based on derivatives may have better properties. Our modifications of HA include the following:
Modification of the carboxyl group
The amide bond is formed by condensing the carboxyl group with the amino group using reagents (carbonyldiimidazole, carbodiimides).
Modification of the hydroxyl group
It is divided into four types: ether formation, ester formation, hemiacetal formation, and oxidation.
Modification of the amide group
It includes modification, hemiacetylation, etc.
Publication Data
Technology: Solid-state crosslinking reaction
Journal: Carbohydrate Polymers
IF: 6.044
Published: 2018
Results: In this study, a method to synthesize HA-based hydrogels is designed. Aqueous mixtures containing different concentrations of Gantrez S97 (GAN) and 5% HA were used to prepare hydrogels. Dynamic scanning calorimetry and infrared spectroscopy detection verified the formation of this crosslink. The method does not use task potentially toxic substances or organic solvents. This study provides a potential method for the preparation of HA-based hydrogels with immobilized forms.
Fig.3 Synthesis and characterization of HA-based hydrogels. (Larrañeta, et al., 2018)
Applications
- HA-based hydrogels are widely used for the research of cartilage damage repair purposes.
- HA-based hydrogels are widely used for the research of drug delivery, cell therapy, and diagnostic purposes.
- HA-based hydrogels are used in research for targeted cancer therapy.
Advantages of Us
- We perform a comprehensive design of HA-based hydrogels, including their biofunctional degradability, rheological properties, chemical structure, composition, resistance to enzymatic degradation, and mechanical integrity.
- We have a strict quality monitoring system and advanced instrumentation to control the preparation of HA-based hydrogels.
- We conduct comprehensive tests on the mechanical properties, biocompatibility, and degree of derivatization of the prepared hydrogels.
CD BioGlyco is committed to providing rational solutions for the development of a wide range of biomaterials. We will surely do our best to overcome all kinds of difficulties and fulfill our client's needs. Please feel free to contact us for inquiries about the design and preparation of HA-based hydrogels. We look forward to your choosing our HA-based tissue gel development services.
