Alginate Characterization Service

Alginate Characterization Service

Precise, Efficient, and Perfect Characterization of Alginate

The unique physical and chemical properties of marine carbohydrates make them widely used in the development of medical materials such as wound dressings, drug delivery, and tissue engineering, so it is extremely important to characterize marine carbohydrates. CD BioGlyco has developed a comprehensive Marine Glycobiology platform to provide clients with a range of services such as Extraction, Characterization, and application development. Our goal is to meet all your needs in marine carbohydrate research.

Alginate is a natural polysaccharide found in the cell walls of brown algae and is widely used in various industries such as pharmaceutical, food, textile, and biomedicine due to its biocompatibility, gel-forming ability, and versatility. Proper characterization of alginate is essential to understand its properties and ensure its quality in specific applications.

Commonly Technologies for Alginate Characterization

  • Nuclear magnetic resonance (NMR): NMR is a key technique used in the characterization of alginate. It not only determines the precise arrangement of monomer units in alginate polymers but also identifies and quantifies different functional groups and monosaccharide residues in alginate samples, providing information about the 3D conformation of alginate chains in solution.
  • Fourier transform infrared spectroscopy (FTIR): FTIR processes the interaction of infrared radiation with samples to provide molecular-level information. FTIR is used to identify specific functional groups within alginate polymers, evaluate the composition and chemical structure of alginate, assess purity, analyze types of chemical bonds, etc.
  • High-performance liquid chromatography (HPLC): HPLC is used to ensure the purity, quality, monomer composition, and molecular weight distribution of the alginate samples.
  • X-ray diffraction (XRD): XRD determines the crystal structure of the material by observing the diffraction pattern of X-rays passing through a sample. It helps determine the crystallinity of alginate and provides detailed information about the molecular arrangement and crystal structure.

Alginate Analysis Service

  • Physiochemical properties of sodium alginate: Estimation of pH and color, determination of moisture content, and estimation of viscosity.
  • Qualitative analysis of sodium alginate: Test for flavonoids, alkaloids, tannins, phlorotannins, terpenoids, steroids, saponins, glycosides, and phenolic.
  • Biochemical analysis of sodium alginate: Carbohydrates analysis, total proteins analysis, lipid analysis, and ash analysis.

Technologies and services for alginate characterization. (CD BioGlyco)

Publication

Technology: UV-vis, NMR, FTIR, thermogravimetric analysis (TGA), XRD

Journal: Scientific reports

IF: 3.8

Published: 2021

Results: The authors extracted alginate from the Egyptian brown algae Sargassum latifolium and determined its chemical composition. The results showed that S. latifolium contained total sugars (41.08%), uronic acid (47.46%), protein (4.61%), lipids (1.13%), water (2%), and sulfate (0.09%). The authors then performed UV-vis, NMR, FTIR, and TGA on the alginate extract. The diffraction pattern of the alginate extract obtained by XRD showed that its crystallinity index was 0.334. The authors also evaluated the growth criteria of Chlorella vulgaris on alginate hydrolysate supplementation and the potential of alginate carriers mixed with Arabic gum for riboflavin drug delivery in simulated gastric fluid.

Fig.1 NMR of sodium alginate extracted from Sargassum latifolium.Fig.1 NMR spectrum of extracted sodium alginate. (Dalal, et al., 2021)

Applications

  • The special biocompatibility and biodegradability of alginate allow it to be made into hydrogels, which are used in wound dressing materials, wastewater treatment, removal of heavy metals in polluted water, etc.
  • Alginate is not only used as a matrix for tissue culture but also to develop vaccine adjuvants, drug delivery carriers, etc. for antibacterial and antiviral treatment.
  • Alginate has thickening and gelling properties, which makes it used in the development of food additives to improve the rheology, water binding capacity, stable emulsions, and film-forming properties of food.
  • Sodium alginate is used as a thickener and stabilizer in the textile and papermaking industries.

Advantages of Us

  • We provide clients with comprehensive alginate characterization services using advanced analytical techniques such as NMR, HPLC, etc. to ensure the accuracy of analytical results.
  • We have an excellent team led by PhDs in the field of glycobiology who have extensive experience in the analysis and characterization of polysaccharides to ensure accurate and insightful analysis.
  • Efficient processing and analysis means faster delivery of results, minimizing project cycles while ensuring quality.
  • Provide clear and detailed project reports, including analytical methods, analytical data, spectra, etc.

Frequently Asked Questions

What information does NMR provide about alginate?

NMR is a powerful analytical technique for characterizing alginate, providing a lot of detailed information about alginate, such as alginate purity as well as potential impurities, bonding patterns of alginate molecules, estimation of the degree of polymerization of alginate, identification of the types of monosaccharides present in alginate, block structure of alginate, etc.

Why determine the ratio of D-mannuronate to L-guluronate (M/G ratio) in alginate?

We use NMR methods to help clients quantify the M/G ratio in alginate. The M/G ratio affects the gelation properties, viscosity and overall physical characteristics of alginate. Understanding the M/G ratio helps with quality control and ensures the consistency of alginate-based products. In addition, the biological activity of alginate including its interaction with cells and biomolecules may be affected by the M/G ratio.

CD BioGlyco performs detailed and accurate marine carbohydrate characterization services to meet the specific needs of researchers and industrial clients. If you would like to access our characterization services and expertise, please feel free to contact us.

Reference

  1. Dalal, S.R.; et al. Characterization of alginate extracted from Sargassum latifolium and its use in Chlorella vulgaris growth promotion and riboflavin drug delivery. Scientific reports. 2021, 11(1): 16741.
For research use only. Not intended for any clinical use.
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