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The unique physical and chemical properties of marine carbohydrates make them widely used in the development of medical materials such as wound dressings, drug delivery, and tissue engineering, so it is extremely important to characterize marine carbohydrates. CD BioGlyco has developed a comprehensive Marine Glycobiology platform to provide clients with a range of services such as Extraction, Characterization, and application development. Our goal is to meet all your needs in marine carbohydrate research.
Alginate is a natural polysaccharide found in the cell walls of brown algae and is widely used in various industries such as pharmaceutical, food, textile, and biomedicine due to its biocompatibility, gel-forming ability, and versatility. Proper characterization of alginate is essential to understand its properties and ensure its quality in specific applications.
Technology: UV-vis, NMR, FTIR, thermogravimetric analysis (TGA), XRD
Journal: Scientific reports
IF: 3.8
Published: 2021
Results: The authors extracted alginate from the Egyptian brown algae Sargassum latifolium and determined its chemical composition. The results showed that S. latifolium contained total sugars (41.08%), uronic acid (47.46%), protein (4.61%), lipids (1.13%), water (2%), and sulfate (0.09%). The authors then performed UV-vis, NMR, FTIR, and TGA on the alginate extract. The diffraction pattern of the alginate extract obtained by XRD showed that its crystallinity index was 0.334. The authors also evaluated the growth criteria of Chlorella vulgaris on alginate hydrolysate supplementation and the potential of alginate carriers mixed with Arabic gum for riboflavin drug delivery in simulated gastric fluid.
Fig.1 NMR spectrum of extracted sodium alginate. (Dalal, et al., 2021)
What information does NMR provide about alginate?
NMR is a powerful analytical technique for characterizing alginate, providing a lot of detailed information about alginate, such as alginate purity as well as potential impurities, bonding patterns of alginate molecules, estimation of the degree of polymerization of alginate, identification of the types of monosaccharides present in alginate, block structure of alginate, etc.
Why determine the ratio of D-mannuronate to L-guluronate (M/G ratio) in alginate?
We use NMR methods to help clients quantify the M/G ratio in alginate. The M/G ratio affects the gelation properties, viscosity and overall physical characteristics of alginate. Understanding the M/G ratio helps with quality control and ensures the consistency of alginate-based products. In addition, the biological activity of alginate including its interaction with cells and biomolecules may be affected by the M/G ratio.
CD BioGlyco performs detailed and accurate marine carbohydrate characterization services to meet the specific needs of researchers and industrial clients. If you would like to access our characterization services and expertise, please feel free to
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