High-purity Holothurins Verified by NMR and Mass Spectrometry for Advanced Research Applications
CD BioGlyco offers holothurin production services, centering on bioactive glycosides sourced from sea cucumbers such as Stichopus japonicus. Known for their strong anticancer and antimicrobial effects, holothurins are triterpene glycosides featuring a triterpene aglycone core, or holostane skeleton, connected to one or more sugar moieties, including glucose, quinovose, and 3-O-methylglucose. Often, sulfate groups are attached to these sugar units, enhancing their bioactivity. The production process involves extracting Holothurins from sea cucumber body walls using organic solvents, followed by purification through advanced chromatography techniques to ensure high purity and bioactivity. The integrity and composition of the final product are verified via nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). Our detailed and careful solutions ensure that high-quality Marine Glycosides are consistently provided for our clients.
Extraction
The body walls of sea cucumbers are processed using a solvent extraction method. Organic solvents, typically methanol or ethanol, are used to efficiently extract holothurins along with other bioactive substances.
Purification
The initial crude extract containing holothurins is subjected to fractionation to concentrate the glycosides. This fraction is then purified through advanced chromatographic methods such as high-performance liquid chromatography (HPLC). This stage is critical for isolating holothurins in their purest form, free from impurities.
Structural Verification
NMR spectroscopy is employed to verify the structure of the purified holothurins, ensuring that their chemical composition is accurate.
MS is also used to confirm the molecular weight and purity of the holothurins, providing an additional layer of quality assurance.
Quality Control
The final holothurin products undergo rigorous quality control tests to confirm their purity and bioactivity. These tests ensure that the holothurins meet the high standards required for research uses.
Publication
DOI: 10.3390/molecules25112471
Technology: NMR, MS
Journal: Molecules
IF: 4.2
Published: 2020
Results: Holothurins, specifically, are bioactive glycosides characterized by their potent anticancer and antimicrobial properties. These compounds are derived from the body walls of sea cucumbers through a process that involves extraction using organic solvents, followed by purification using advanced chromatographic techniques. Quality control and structural verification are conducted using NMR spectroscopy and MS to ensure the purity and bioactivity of the isolated compounds.
Applications
- Holothurins have demonstrated significant potential in inhibiting the growth of cancer cells, making them valuable for developing new cancer treatments.
- Holothurins possess antimicrobial properties, effective against a range of bacterial and fungal pathogens.
- The unique structure and bioactivity of holothurins make them suitable for identifying new biomarkers in disease progression and treatment response.
Advantages
- CD BioGlyco employs state-of-the-art purification methods, such as HPLC, to ensure the high purity of holothurins.
- CD BioGlyco's service is scalable, accommodating both small-scale research projects and larger industrial applications, providing versatility to meet diverse research needs. We also provide so many Other Marine Biomolecules for clients to choose from.
- By focusing on marine-derived glycosides like holothurins, CD BioGlyco contributes to the innovative exploration of natural products in drug discovery and development.
CD BioGlyco's holothurin production service is your ideal choice if you are looking for high-quality, pure holothurins for your research. With advanced purification techniques, rigorous quality control, and a team of experts dedicated to marine natural products, we guarantee reliable and effective compounds tailored to your specific research needs. Please feel free to contact us for more details and a personal quotation.
Frequently Asked Questions
What sea cucumber species does CD BioGlyco use for Holothurin extraction?
CD BioGlyco primarily uses the sea cucumber species Stichopus japonicus for extracting holothurins, known for its high content of these bioactive compounds.
How can I start using CD BioGlyco's Holothurin production service?
To begin, contact CD BioGlyco through our website or customer service. We will guide you through the process, discuss your specific needs, and provide a customized solution for Holothurin production.
References
- Luparello, C.; et al. Collective locomotion of human cells, wound healing and their control by extracts and isolated compounds from marine invertebrates. Molecules, 2020, 25(11): 2471.
- From Wikipedia: https://en.wikipedia.org/wiki/Column_chromatography#/media/File:Column_chromatography_sequence.png.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Agilent1200HPLC.jpg.
