Relying on an Efficient Team to Deliver High-purity Variegatuside
Variegatuside is a non-sulfated triterpene glycoside. It is a metabolite isolated from sea cucumbers. CD BioGlyco uses a variety of combination techniques to purify and isolate various types of glycosides. Our high-quality variegatusides greatly facilitate your projects in the fields of biopharmaceuticals, food research, and biomedicine. We offer individualized production solutions based on our clients' needs for their samples. Whether you need large or small batches, we are the right partner for you!
Raw material pretreatment
Washing and crushing: Our laboratory dusts and cleans the collected sea cucumbers. The dried sea cucumbers are processed into a powder suitable for extraction by mechanical crushing equipment.
Extraction and Isolation
- Organic solvent extraction: We repeatedly extract the sea cucumber at least three times at room temperature using ethanol. Our researchers suspend the ethanol extract in water and then separate it with petroleum ether and n-BuOH, respectively.
- Chromatography purification: Our common separation techniques include silica gel chromatography, reversed-phase abbr high-performance liquid chromatography (RP-HPLC), thin layer chromatography (TLC), and size exclusion chromatography (SEC). It is difficult to completely purify a single component with a single technique, so we offer a combination of techniques to achieve high-purity separations.
Characterization
- Basic testing: Our lab provides professional purity testing by HPLC. We provide accurate determination of monosaccharide composition by gas chromatography-mass spectrometry (GC-MS).
- Structure analysis: We use nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS) technology to analyze the molecular weight and structure of purified variegatuside.
- Bioassay: We measure the antifungal activity of variegatusides using a variety of strains. The strains we provided include but are not limited to Candida albicans, Candida parapsilosis, Cryptococcus neoformans, Candida tropicalis, Candida pseudotropicalis, and Microsporum gypseum. Our researcher use an automatic microplate reader instrument in the determination of optical density (OD) to evaluate its antifungal activity.
Publication
Technology: NMR spectra, High-resolution electrospray ionization mass spectrometry (HR-ESI-MS), GC-MS, TLC, HPLC, SEC
Journal: Marine Drugs
Published: 2014
IF: 4.9
Results: In this work, researchers extracted a total of seven glycosides from sea cucumber, including four novel non-sulfated triterpene glycosides variegatuside C-F and three known glycosides. The researchers characterized the structures of the glycosides by NMR and ESI-MS. In vitro activity tests showed that all of these trip glycosides have very significant antifungal activity. This study provides valuable information on antifungal natural products.
Fig.1 The structures of seven glycosides. (Wang, et al., 2014)
Applications
- Variegatuside provides valuable insights into the bioactive components of sea cucumbers and their pharmacological effects.
- Antimicrobial activity: Variegatuside has an inhibitory effect on pathogenic bacteria and can be used to develop antimicrobial drugs.
- Antioxidant activity: Variegatuside has significant antioxidant activity, neutralizing free radicals and protecting cells from oxidative damage.
Advantages of Us
- CD BioGlyco offers many types of variegatuside production services, including mixtures and monomers such as variegatuside A, variegatuside B, variegatuside C, variegatuside D, variegatuside E, variegatuside F, variegatuside G, variegatuside H, and variegatuside I.
- Before extraction, our researchers use methanol to dissolve the extract multiple times and filtration to remove insoluble matter.
Frequently Asked Questions
What are the advantages of extracting sea cucumber-derived saponins by alcohol solutions?
Saponins are usually soluble in water and alcohol solutions, and insoluble in ether, chloroform, and benzene. Alcohol extraction methods commonly used extracts are methanol, ethanol, n-butanol, and so on. Currently, ethanol is the most common solvent used for the extraction of sea cucumber saponins, and 60% and 70% ethanol systems are the most common, which is hypothesized to be related to the structure of sea cucumber saponins with amphiphilicity.
What are the methods included in the determination of sea cucumber saponin content?
Commonly used methods include a colorimetric method, TLC, and HPLC. Colorimetric methods have the advantages of simplicity and speed. TLC uses a certain unfolding agent to unfold and then combines it with the use of other instruments for quantitative determination. HPLC effectively separates the sample and impurities, in the separation and determination of saponin is more often used.
Relying on excellent technology and quality service, CD BioGlyco provides high-quality variegatuside to clients worldwide. Our company always puts the needs of our clients first. We are committed to providing our clients with high-efficiency, high-quality, and high-purity marine glycosides. Whatever your concerns, please feel free to contact us for help!
Reference
- Wang, X.H.; et al. Variegatusides: New non-sulphated triterpene glycosides from the sea cucumber Stichopus variegates semper. Marine Drugs. 2014, 12(4): 2004-2018.
