Provide Efficient Echinoside A Production Services for Clients on the Pragmatic Principle
CD BioGlyco is a service company specializing in providing Marine Biomolecules. Whether our clients need Marine Carbohydrates, Marine Glycoproteins, Marine Lectins, or Marine Glycosides, we provide high-quality production and characterization services. We have advanced technology and skilled staff to provide a one-stop service for echinoside A production, characterization, activity analysis, and data processing.
Extraction and Purification of Echinoside A
CD BioGlyco provides highly efficient echinoside A extraction services by ethanol extraction. Our researchers repeatedly use ethanol extraction several times at room temperature to ensure high yields. We perform small-batch extractions using different concentrations of ethanol to screen for optimal extraction concentrations.
Our researchers carry out the preliminary separation of the crude product by different concentrations of eluent in the resin column. Then we purify it sequentially by silica gel column, octadecylsilyl (ODS) cartridge, and high-performance liquid chromatography (HPLC) to obtain high-purity echinoside.
Echinoside A Characterization
CD BioGlyco provides professional echinoside A analysis services including gas chromatography-mass spectrometry (GC-MS) analysis, nuclear magnetic resonance (NMR) spectroscopy analysis, Fourier-transform-infrared-spectroscopy (FTIR) analysis, and electrospray ionization-mass spectrometry (ESI-MS) analysis.
For purified echinosides, we offer purity testing, yield testing, and physicochemical property analysis services.
Bioactivity Analysis of Echinoside A
Testing for echinoside A activity is an important item that should not be overlooked. Our well-trained team offers the following activity analysis services:
- Cell counting kit-8 (CCK-8) assay
- Reactive oxygen species assay
- Apoptosis assay
- Mitochondrial membrane potential assay
- Cell cycle assay
- Caspase-3 assay
Special Service
- We support custom purity and specification of echinoside A production services.
- For all analytical characterization data, CD BioGlyco provides raw data packages and professionally analyzed data reports.
- Our lab offers extraction and isolation method optimization services. Each experimental data is carefully documented and delivered.
- If you need specific extraction and purification methods, please provide us with the relevant information.
- Our researchers regularly report to clients on the progress of their projects and maintain communication at all times.
Publication
Technology: HPLC, In vivo pharmacokinetics, Cell model
Journal: Marine Drugs
Published: 2016
IF: 4.9
Results: In this study, researchers isolated and purified echinoside A and holotoxin A1. The researchers determined the pharmacokinetic profile and bioavailability of these two sea cucumber saponins. Echinoside A was found to have significant cell permeability and intestinal absorption. This study focused on the relationship between the structure of sea cucumber saponins and their bioavailability.
Fig.1 ESI-MS spectra of echinoside A. (Li, et al., 2016)
Applications
- Echinoside A can be used as a potential target for anti-breast cancer drug development to improve clinical efficacy.
- Echinoside A has potent antimicrobial activity and provides valuable value in the development of antimicrobial drugs.
- The high purity and well-defined structure of echinoside A helps drive deeper pharmacological activities in marine glycobiology.
- Echinoside A can be applied to the development of new marine-derived Top2α inhibitors.
- Inhibition of tumor metastasis: EA has significant tumor metastasis inhibitory effects, acting through the MMP-9 signaling pathway and further inhibiting vascular endothelial growth factor proteins.
Advantages of Us
- CD BioGlyco has a highly responsible marine molecule production team to efficiently complete glycoside production.
- Our researchers have accumulated many years of experience in extraction and isolation through multiple extractions and combined separations to ensure high-quality deliveries.
- Our extraction and isolation methods are gentle and do not destroy the structure and pharmacological activity of the target.
- We always comply with a strict quality system to ensure the quality of our products and services.
Frequently Asked Questions
What monosaccharides does echinoside A contain?
Echinoside A contains 4 monosaccharides D-xylose, D-quinovose, D-glucose, and D-3-O-methylglucose. Unusually, there is a sulfate group on the C-4 of the first xylose residue.
What biological activities do echinoside A have?
Echinoside A exhibits a variety of biological activities such as anti-obesity, anti-tumor, stimulation of hepatic fatty acid β-oxidation, and inhibition of fatty acid biosynthesis.
CD BioGlyco has a well-trained team to provide clients with satisfactory echinoside A production. Echinoside A has strong anti-tumor activity in vivo or in vitro, and we have a comprehensive range of cellular and mouse models to meet clients' applications in activity testing. We always maintain a passionate and professional philosophy to serve every client. No matter what problems you encounter in your echinoside A production, please feel free to contact us.
Reference
- Li, S.; et al. Absorption and transport of sea cucumber saponins from Apostichopus japonicus. Marine Drugs. 2016, 14(6): 114.
