Help Explore the Value of Marine Collagen Through Efficient Production
Marine collagen has a variety of biochemical functions that affect cell growth and has important applications in biomaterials, cosmetics, etc. CD BioGlyco provides a one-stop marine collagen production service based on highly efficient extraction processes and advanced analytical techniques. We are committed to ensuring client satisfaction from initial communication, and production to product delivery.
We have extensive experience in the production of Marine Glycoproteins and other Marine Biomolecules. Our experienced specialists develop individualized production plans based on the type of raw material and client research needs. The production process is described below:
Extraction
This process involves the chemical pretreatment of raw materials to remove non-collagenous proteins, colors, or fats and the extraction of collagen. We extract collagen from marine organisms such as sponges, jellyfish, fish, squid, etc., using salt precipitation, acid solubilization, or enzymatic solubilization combined with a series of physical and mechanical treatments. We optimize factors such as extraction temperature, time, solvent concentration, and solid-liquid ratio to ensure high collagen recovery.
- Salt precipitation method isolation
- Acid-soluble method isolation
- Enzyme-soluble method isolation
Purification
The extracted material is purified by precipitation, centrifugation, and dialysis. High-quality marine collagen is finally obtained by freeze-drying.
Characterization
We combine high-performance liquid chromatography, mass spectrometry, and other methods to characterize the chemistry, structure, and properties of the marine collagen obtained from purification, including the following:
- Chemical composition of collagen: Fourier-transform infrared spectroscopy (FTIR) is used as a tool to analyze the chemical composition of collagen.
- Collagen purity characterization and molecular weight distribution: Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to identify protein patterns and determine the molecular weight distribution of collagen peptides.
- Secondary structure of collagen: We analyze the secondary structure, binding, and folding properties of collagen by circular dichroism.
- Collagen yield and amino acid analysis: We measure total protein content and use chromatography to separate, identify and quantify amino acids.
- Thermal stability: We analyze the thermal properties of collagen by differential scanning calorimetry (DSC).
- In addition, we analyze the high-level structure of collagen.
Publication Data
Technology: Acid extraction, supercritical fluid extraction, FTIR, SDS-PAGE, DSC
Journal: Polymers
IF: 4.329
Published: 2020
Results: This paper describes several methods for isolating and characterizing collagen from fish. Depending on the marine source, different extraction methods can be used, including acid extraction, supercritical fluid (SF) extraction, and others. A variety of factors affect the efficiency of collagen extraction. In addition to the extraction methods, this paper describes in detail some of the techniques used to analyze the molecular weight, structure, etc. of collagen. These methods support the development of production schemes for collagen from different marine organisms.
Fig.1 The process of extracting collagen from fish. (Jafari, et al., 2020)
Applications
- High-quality marine collagen is water-soluble, metabolically compatible, and is used as a preparation of various biomaterials.
- High-quality marine collagen is a multifunctional compound used in research related to the treatment of skin damage of varying severity.
- High-quality marine collagen aids in the absorption of calcium and zinc and is used in bone and cartilage regeneration studies.
Advantages
- We consider the effects of multiple factors on marine collagen yield and optimize the extraction process.
- We combine a variety of advanced analytical techniques to characterize the structure and properties of collagen.
- In addition to high-quality marine collagen products, we also guide collagen research and application development.
Frequently Asked Questions
What are the advantages of marine collagen?
Sponges, fish, squid, jellyfish, octopus, and other marine organisms are a great source of collagen with great advantages. They are free of animal pathogens, abundant in origin, and metabolically compatible, making them a simpler, more promising, and safer alternative to be used in the preparation of biomaterials, biomedicine, cosmetics, and other fields.
What factors affect the yield of marine collagen?
Solvent concentration, time, solid-liquid ratio, and temperature all affect the extraction rate of marine collagen. The impact of each parameter should be considered and optimized during production to determine the appropriate range.
- Temperature: The extraction temperature depends on the type of marine collagen extraction substrate.
- Extraction time: A shorter extraction time will result in too low a yield of marine collagen, but too long may result in degradation of the leached peptide. Optimal extraction time needs to be determined by optimization experiments.
- Solvent Concentration: Excessive solvent concentration can lead to peptide degradation, thus reducing the yield and purity of marine collagen.
- Solid-liquid ratio: The molecular size of marine collagens largely determines their function and application. The solid-liquid ratio needs to be adjusted according to the research and application during the production process.
CD BioGlyco ensures the structural integrity and activity of marine collagen through efficient production programs. Our team is responsive to client inquiries, feedback, and specific requests. Please feel free to contact us for further details on marine collagen, should this be of interest to you.
Reference
- Jafari, H.; et al. Fish collagen: Extraction, characterization, and applications for biomaterials engineering. Polymers. 2020, 12(10): 2230.
