Discover the Hidden Depths of Mucin-type Glycoprotein with Our High-quality Production Service
CD BioGlyco is an outstanding company that produces Marine Biomolecules including Marine Glycoproteins and improves a high-quality mucin-type glycoprotein production service. We extract, purify, and characterize mucin-type glycoproteins from different marine organisms, thus helping our clients to better understand their structures and their potential applications in various fields such as biomedicine, cosmetics, and materials.
We are always at the forefront of marine glycobiology and are committed to serving our clients with efficient production services and high-quality mucin-type glycoprotein products. The production scheme of mucin-type glycoprotein from different sources has been optimized. The specific production steps are as follows:
Extraction
We isolate mucin-type glycoprotein from mucin-rich marine organisms, such as mollusks, jellyfish, and brittle stars, using ultrasonic extraction, etc.
Purification
After tissue homogenization and extraction, the mucin-type glycoprotein is further isolated and purified by centrifugation, dialysis, enzymatic digestion, size exclusion chromatography, hydrophobic interaction chromatography (HIC), etc.
- Centrifugation, dialysis, and enzymatic digestion are used to remove impurities such as pigments, salts, and non-mucin-type proteins.
- Size exclusion chromatography separates mucin-type glycoproteins based on their size and molecular weight.
- Depending on the hydrophobicity of the mucin-type glycoprotein surface, HIC utilizes the reversible interaction between the mucin-type glycoprotein and the hydrophobic groups to achieve the separation effect.
Characterization
We have a variety of advanced identifiers, such as gas chromatography-mass spectrometry (GC-MS), mass spectrometry, etc., to characterize the structure and properties of mucin-type glycoprotein, including the following:
- Protein concentration
- Total sugar content
- Molecular weight
- Monosaccharide composition analysis: Monosaccharide composition, linkage sequence, conformation, etc.
- Amino acid composition
- Adhesion capacity
Publication Data
Technology: HIC chromatography
Journal: Bioscience Methods
Published: 2011
Results: In this study, mucin was isolated and purified from blue blubber jellyfish and characterized. The results showed that the molecular weight in the mucin was 120-300 kD and had a high carbohydrate content, with N-acetylglucosamine, galactose, and N-acetylgalactosamine as the main components. The mucin was also found to have strong antimicrobial adhesion activity by comparison with other mucins. This study provides methodological support for our isolation, purification, and characterization of marine mucin-type glycoprotein.
Fig.1 Steps in the extraction and purification of jellyfish mucin. (Pearson, et al., 2011)
Applications
- The mucin-type glycoprotein is used to study defense mechanisms and intermolecular interactions in marine organisms.
- With its highly viscous and lubricating, the mucin-type glycoprotein is used in biomaterials to effectively protect coated substances.
- With its moisturizing properties, mucin-type glycoprotein has potential applications in cosmetics and skin care products.
Highlights
- Our research team has extensive knowledge and experience in marine mucin-type glycoprotein production and research. We use advanced technology and continuously optimize our production process to ensure high-quality products.
- We offer customized mucin-type glycoprotein production solutions to best meet our client's research needs and improve yield and product quality.
- We incorporate a variety of gentle methods such as enzymatic digestion and HIC to maximize the integrity and activity of the mucin-type glycoprotein.
Frequently Asked Questions
What is the current concentration of research on marine mucin-type glycoprotein?
Research on marine mucin-type glycoprotein has focused on arthropods such as shrimp and crab, echinoderms such as jellyfish and starfish, mollusks such as jellyfish, etc. With properties such as high viscosity and lubrication, these mucin-type glycoproteins are used in the study of innate defense mechanisms and other physiological processes in animals. In addition to this, researchers are developing them for biotechnological and biomedical applications such as bioadhesives, coatings, tissue engineering, etc.
What is the function of mucin-type glycoprotein in marine animals?
The mucin-type glycoprotein is well characterized and involved in a variety of physiological activities that are essential for maintaining mucosal integrity and function. It and its interacting substances mediate a variety of biological processes to enhance tissue integrity, physical protection, and defense.
CD BioGlyco provides innovative production solutions and high-quality products for researchers and companies specializing in mucin-type glycoproteins. Please feel free to contact us to discuss further production details if you are interested in this area or need it.
Reference
- Pearson, R.; et al. Isolation, biochemical characterization and anti-adhesion property of mucin from the blue blubber jellyfish (Catostylus mosaicus). Bioscience Methods. 2011, 2.
