Trust Our Marine Mannoglucan Characterization Service for Cutting-edge Analysis and Innovative Insights
At CD BioGlyco, we are deeply involved in the field of marine biotechnology and are committed to providing cutting-edge Marine Carbohydrate Characterization Services to global clients. As an important branch of this comprehensive service framework, we specifically provide Marine Plant Polysaccharide Characterization Services for marine plant resources, aiming to deeply analyze the complex structure and unique functions of these plant polysaccharides from the vast ocean. In this special service, mannoglucan characterization service is one of the key services, which focuses on comprehensive and accurate characterization analysis of marine mannoglucan, from molecular structure to biological functional characteristics.
Monosaccharide Composition Analysis
- High-performance liquid chromatography (HPLC): We utilize HPLC to determine the precise monosaccharide composition of the marine mannoglucans. This process involves hydrolyzing the polysaccharides into their monosaccharide components, which are then separated and quantified.
- Gas chromatography-mass spectrometry (GC-MS): By transforming the monosaccharides into volatile derivatives, GC-MS provides a highly sensitive method to identify and quantify the individual glycans present in the mannoglucan samples.
Molecular Weight Determination
- Size exclusion chromatography (SEC): SEC is employed to determine the molecular weight distribution of marine mannoglucans. This technique separates molecules based on size, providing vital information about the polymer distribution.
- Multi-angle laser light scattering (MALLS): When coupled with SEC, MALLS offers an accurate measure of the molecular weight and radius of gyration, leading to a comprehensive understanding of the polymer structure.
Structural Analysis
- Nuclear magnetic resonance (NMR) spectroscopy: NMR is pivotal in elucidating the detailed structural features of mannoglucans, including linkage types and anomeric configurations. This technique provides a molecular-level insight into the arrangement of monosaccharides.
- Fourier-transform infrared (FTIR) spectroscopy: FTIR is used to characterize functional groups and identify characteristic absorption bands related to specific bonds within the mannoglucan structure.
- Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry: This technique allows us to analyze the mass-to-charge ratio of ionized polymer fragments, offering insights into the repeat units and possible modifications in the mannoglucans.
Rheological Property Assessment
- Viscometry: We assess the viscosity of marine mannoglucan solutions to understand their flow behaviors, which is crucial for applications where gelation and thickening properties are essential.
- Dynamic mechanical analysis (DMA): DMA offers insights into the mechanical properties of mannoglucan-based hydrogels, such as their elasticity and stress resistance.
Functional Property Assessment
- Bioactivity assays: We conduct in vitro and in vivo assays to evaluate the specific biological activities of marine mannoglucans, including their anti-inflammatory, antiviral, and immunomodulatory effects.
- Thermal analysis: Techniques like differential scanning calorimetry (DSC) are used to assess thermal stability and transitions, providing critical data for processing and application development.
Publication
Journal: Marine drugs
IF: 4.9
Published: 2013
Results: The authors aimed to explore the potential of Bullacta exarata in medical applications by isolating and purifying a mannoglucan, designated as BEPS-IB, from the foot muscle of B. exarata following papain digestion. Chemical composition analysis revealed that BEPS-IB primarily comprised glucose and mannose in a molar ratio of 1:0.52, with an approximate molecular weight of 94 kDa. Methylation analysis was used to determine the linkage information, while IR and 2D NMR confirmed the anomeric configuration and chain linkage. The findings showed that BEPS-IB consisted of a Glcp6Manp heptasaccharide repeating unit in the backbone, with occasional mannose branch chains (14%) attached to the backbone mannose. Further antioxidant assays demonstrated that BEPS-IB exhibited positive antioxidant activity, effectively scavenging superoxide radicals and exhibiting reducing power.
Fig.1 Antioxidant activity of the BEPS-IB. (Liu, et al., 2013)
Applications of Marine Mannoglucan
- Thickener and stabilizer in the food industry: Marine mannan can be used as a thickener, suspending agent, and stabilizer due to its high viscosity and thermal stability.
- Preparation of gel food: Marine mannan can be used to make gel food.
- Development of food packaging materials: Marine mannan can be used to develop new food packaging materials. Such materials may have excellent barrier properties, degradability, and biocompatibility.
Advantages
- Our advanced analytical technology platform ensures detailed structural and functional characterization of marine mannoglucan.
- We flexibly adjust our service process to ensure that the personalized needs of our clients are met to the greatest extent possible.
- We have a professional team of experienced biologists, chemists, and technical experts. They have rich experience in marine biological research and provide clients with professional technical consultation and solutions.
Frequently Asked Questions
What is marine mannoglucan? Why is it important in the biotechnology field?
Marine mannoglucan is a polysaccharide compound derived from the ocean or fermented by specific microorganisms, with excellent physical and chemical properties such as thickening, suspension, and stabilization. In the field of biotechnology, Marine Mannoglucan is widely used in the food, medicine, cosmetics, and petroleum industries. As a thickener and stabilizer, it can improve the texture and stability of products, and improve the sensory experience and shelf life of products. In the pharmaceutical field, it can also be used as a drug carrier to improve the bioavailability and targeting of drugs.
What are the differences in the dosage and effect of marine mannoglucan in different application fields?
The dosage and effect of marine mannoglucan do vary in different application fields. For example, in the food industry, as a thickener, its dosage is usually low, which can effectively improve the taste and texture of food. In the pharmaceutical field, as a drug carrier, its dosage and effect depend on the specific drug and formulation design. Our service provides personalized dosage recommendations and optimization solutions based on the specific needs and application scenarios of clients.
At CD BioGlyco, our marine mannoglucan characterization service uses a series of advanced and comprehensive characterization strategies to provide clients with detailed and accurate structural and functional information. We are always client-centric and committed to promoting the in-depth development and utilization of marine biological resources. Please feel free to contact us for more details if you are interested in our marine mannoglucan characterization service!
Reference
- Liu, D.; et al. Isolation and structural characterization of a novel antioxidant mannoglucan from a marine bubble snail, Bullacta exarata (Philippi). Marine drugs. 2013, 11(11): 4464-4477.
