Marine Plant Polysaccharide Characterization Service

Marine Plant Polysaccharide Characterization Service at CD BioGlyco

Marine polysaccharides are rich in resources, and their structures are diverse due to their special living environment. Marine plant polysaccharides refer to the general name of high-molecular carbohydrates extracted from marine plants. They are macromolecular polymers connected by monosaccharides through glycosidic bonds. They have a variety of physiological activities, such as anti-tumor, antioxidant, anti-viral, immune regulation, etc., and have good application prospects in functional foods, pharmaceuticals, and other fields. CD BioGlyco has been engaged in the research of marine carbohydrates for many years and has accumulated a wealth of knowledge and experience. We have established a world-class marine carbohydrate platform to provide clients with Marine Carbohydrate Extraction, purification, Characterization, Biological Activity Analysis, and corresponding downstream application research based on marine carbohydrates, such as Vaccine Development.

Structural characterization is an important step in the study of marine carbohydrates, helping to elucidate their biological activity mechanisms and discover new biological functions. Using a variety of advanced Technologies, we help clients directly analyze and characterize marine carbohydrates from different plant sources, such as brown, red, and green algae. We also help you start by extracting marine polysaccharides from marine plants and gradually implement purification, structural characterization, biological activity testing, and application development. Our service process is as follows:

Extraction Services for Marine Plant Polysaccharide

The client provides us with the target marine plants, such as brown algae, red algae, and green algae. We use water extraction, ultrasound, microwave, and enzymatic techniques to separate and purify a variety of polysaccharide components from the plants.

Purification Services for Marine Plant Polysaccharide

The extracted polysaccharides require further purification to analyze the structure and better explore their activity. We use ion exchange chromatography to separate neutral sugars and acidic sugars in crude polysaccharides according to different charge properties, solubility, and molecular weight. Then we use precipitation, ultrafiltration, and gel chromatography to further separate polysaccharide components of different molecular weights or sizes.

Characterization Services for Marine Plant Polysaccharide

The structures of marine polysaccharides are often complex, so we use a variety of technologies and biological methods to help clients analyze and characterize marine polysaccharides, such as nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), gas chromatography (GC), high-performance liquid chromatography (HPLC), mass spectrometry (MS).

• Purity of polysaccharides and molecular weight: The chromatography system is a reliable method to determine the molecular weight and distribution of polysaccharides. It obtains accurate molecular weight information of samples and determines polysaccharide geometric orientation and sample recovery rate information.
• Monosaccharide composition: Monosaccharide composition analysis is the basis for research on the structure and function of marine polysaccharides. The accuracy of the results directly affects the efficiency and accuracy of subsequent methylation analysis and NMR spectrum analysis. Among many monosaccharide composition analysis methods, ion chromatography is an ideal detection method because it does not require hydrolysis of the sample before derivatization.
• Fine structure characterization: Various characterization technologies such as NMR and MS have their characteristics. We use relevant analysis methods comprehensively based on client samples to characterize complex marine polysaccharide structures, such as glycosidic bond types, main chain structures, branched chain structures, and other information.

Fig.1 Marine plant polysaccharide characterization service. (CD BioGlyco)

We offer our clients characterization services for the following marine plant polysaccharides:

Publication

Technology: HPLC, FTIR, multi-angle laser light scattering (MALLS), and refractive index detector (RID)

Journal: BMC chemistry

IF: 4.6

Published: 2021

Results: The authors used methanol/dichloromethane/water (4: 2: 1, v/v/v) to prepare crude brown seaweed polysaccharides from brown algae samples, and optimized the oligosaccharide-1-phenyl-3-methyl-5-pyrazolone (PMP) derivatives through a "one-factor-at-a-time" approach. Polysaccharides in different species of brown algae were then characterized and compared based on saccharide mapping of HPLC, FT-IR, MALLS, and RID. In addition, the authors also measured the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical scavenging activity of brown seaweed polysaccharide samples. The results show that brown polysaccharides from different brown algae exhibit good antioxidant activity and are promising as antioxidants in the food, pharmaceutical, and cosmetic industries.

Fig.2 FTIR spectra of brown seaweed polysaccharides samples. (Chen, et al., 2021)

Applications

• Marine plant polysaccharides play an important role in preventing oxidative damage to organisms in vitro as free radical protectants. Polysaccharides extracted from marine plants can be used as antioxidants in health products and pharmaceuticals.
• With the development of modern pharmaceutical technology, seaweed polysaccharides are structurally modified or degraded into active oligosaccharides, thereby exerting greater medicinal value and providing new ideas for the prevention and treatment of cancer.
• Marine plant polysaccharides have high anticoagulant and antiplatelet activities and can be used in drug research in related fields.

Advantages

• Not only do we directly characterize marine polysaccharide samples, but we also extract and purify marine polysaccharides from marine plants and measure their biological activity.
• We determine the purity, molecular weight, and monosaccharide composition of polysaccharides to better assist in the fine structural characterization of polysaccharides.
• Each characterization method has its characteristics. We use relevant analysis methods comprehensively based on the characteristics of client samples to better analyze polysaccharides.

At CD BioGlyco, we continue to research marine glycobiology to help clients improve the preparation efficiency and yield of polysaccharides and combine various special technologies to characterize their structures. If you have any needs in the field of marine glycobiology, please feel free to contact us to discuss the details.