Enzymatic Modification of Marine Carbohydrate

Enzymatic Modification of Marine Carbohydrate at CD BioGlyco

CD BioGlyco offers Modified Marine Carbohydrates by modifying functional groups, lowering molecular weight, and others to improve their biological activity. In addition to the Chemical Modification method and Conjugation with Polymer, we also provide enzymatic modification service for marine carbohydrates. Marine carbohydrates are rich and diverse in origin and are a special kind of bioactive material. Our goal is to improve the functionality and expand the range of applications by modifying a wide range of carbohydrates.

Enzyme Selection

Enzyme modification is a green method compared to the chemical modification method. The enzyme acts as a highly efficient catalyst and its catalytic reaction operates under mild conditions and produces very little waste. Different enzymes catalyze different reactions. We screen specific enzymes based on their ability to catalyze targeted reactions of carbohydrate substrates for precision modification. The enzymes we commonly use include glycosidases and glycosyltransferases, such as lysozyme and papain. Each enzyme has different catalytic activity and specificity.

Structural Modification

We employ specific enzymes to perform various structural modifications on marine carbohydrates, including acetylation, deacetylation, depolymerization, grafting, etc. Such modifications alter the chemical, physical, and biological properties of the carbohydrates, providing the potential for expanded applications. We also attach bioactive molecules to marine carbohydrate backbones utilizing enzymes. This form materials with specific functions. In addition to this, we utilize specific enzymes to selectively cleave specific regions of marine carbohydrates, such as glycosidic bonds. Specific modifications of marine carbohydrates are realized by changing molecular weight and structure.

Enzymes are catalytically specific compared to chemical modification methods. In addition, enzymatic reactions are more environmentally friendly and do not require toxic chemicals or solvents. For this reason, we choose enzymatic modifications for a wide range of marine carbohydrates. We use specific enzymes to alter the structure and properties of marine carbohydrates according to client requirements. Enzymatic modification is used to introduce or modify functional groups of marine carbohydrates in a targeted manner.

We also combine enzymatic modification with other methods to achieve a wider variety of modifications to marine carbohydrates.

Fig.1 Enzymatic modification of marine carbohydrate. (CD BioGlyco)

Publication Data

Technology: Enzymatic modification

Journal: Food Hydrocolloids

IF: 10.7

Published: 2022

Results: In this study, the effect of enzymatic modifications on the supramolecular structure based on polysaccharides was examined. Chitosan and gum arabic were modified by covalent grafting of ferulic acid oxidation products, which led to the establishment of new interactions, such as π-interactions. The results show that the modification can lead to the formation of various supramolecular structures through changes in physicochemical conditions. This study provides a method to control the formation of supramolecular structures through modification.

Fig.2 Enzymatic modifications alter aggregation or coalescence behavior. (Vuillemin, et al., 2022)

Applications

• Enzymatic modifications to control changes in the structure and properties of marine carbohydrates led to the development of novel biomaterials, drugs with therapeutic effects, and functional ingredients.
• Enzymatic modifications are used to form multifunctional materials with controlled release properties based on marine carbohydrates.
• Enzymatic modifications are used to synthesize marine carbohydrates with specific biological activities such as anti-inflammatory, immunomodulatory, or antioxidant effects.

Highlights of Us

• We develop specific modifications according to client requirements and screen enzymes with specific catalytic properties to improve efficiency.
• Our enzymatic modifications of marine carbohydrates include molecular weight and functional group changes, the introduction of biologically active molecules, etc.
• We combine enzymatic reactions with other strategies to broaden the range of modifications that can be achieved, opening up the possibility of developing advanced materials with multifunctional properties.

CD BioGlyco utilizes specific enzymes to achieve specific changes in the properties and functionality of marine carbohydrates. Please feel free to contact us for more information on enzyme screening, enzymatic modification processes, etc., and let us know your needs. We will make sure that all enzymatic modification experiments are completed with excellence.