Marine Glycoenzyme Development Service

Marine glycoenzymes are vital biocatalysts in marine organisms. They participate in a variety of biochemical reactions and play a key role in the growth, development, and metabolism of marine organisms. CD BioGlyco provides a full range of marine glycoenzyme development services, aiming to provide clients with one-stop solutions from design, analysis, and screening to detection. This service is based on computer-aided design and genetic engineering technology, combined with biochemical detection methods to provide a one-stop solution to help clients quickly and accurately develop and optimize marine glycoenzyme.

Fig.1 Marine glycoenzyme development service. (CD BioGlyco)

Marine Glycoenzyme Design Service at CD BioGlyco

This service involves the design and development of novel glycoenzyme derived from marine organisms. We build and optimize glycoenzymes suitable for specific applications using computational modeling, protein engineering techniques, and structure-function relationships. Our expertise includes rational design, directed evolution, and semi-rational approaches to achieve enhanced catalytic activity and substrate specificity.

• Marine Glycoenzyme Rational Design Service
• Marine Glycoenzyme Directed Evolution Design Service
• Marine Glycoenzyme Semi-rational Design Service

Marine Glycosyltransferase Analysis Service at CD BioGlyco

This service focuses on the detailed analysis of glycosyltransferases of marine origin. It involves elucidation of catalytic mechanisms, substrate recognition, and characterization of glycosylation patterns. By employing advanced analytical methods such as mass spectrometry, chromatography, and enzymatic assays, we deciphered the complex roles and functions of these enzymes in glycan biosynthesis.

• Isolation and Purification of Marine Glycosyltransferase
• X-ray Crystallographic Analysis of Marine Glycosyltransferase
• Bioinformatics Analysis of Marine Glycosyltransferase
• Enzyme Activity Analysis of Marine Glycosyltransferase

Marine Glycosidase Analysis Service at CD BioGlyco

Here we delve into the analysis and characterization of glycosidases from marine organisms. This service involves studying their substrate specificity, enzyme kinetics, and structural features. Utilizing a variety of biochemical and biophysical techniques, we provide insights into glycosidase activity, molecular structure, and potential applications in carbohydrate modification.

• Isolation and Purification of Marine Glycosidase
• Crystal Structure Determination of Marine Glycosidase
• Sequence and Phylogenetic Analysis of Marine Glycosidase
• Enzyme Activity Assay of Marine Glycosidase

Marine Glycoenzyme Screening Service at CD BioGlyco

This service involves high-throughput screening methods to identify and select potential marine glycoenzymes suitable for specific applications. We evaluate enzyme activity, stability, and substrate specificity utilizing an innovative screening platform. This facilitates the discovery of promising glycoenzymes with desired catalytic properties.

• Agar Plate-based Marine Glycoenzyme Screening Service
• Microtiter Plate-based Marine Glycoenzyme Screening Service
• FACS-based Marine Glycoenzyme Screening Service
• Chromatography-based Marine Glycoenzyme Screening Service
• Glycophage Display System-based Marine Glycoenzyme Screening Service

Marine Glycoenzyme Detection Service at CD BioGlyco

This service provides sensitive and accurate detection methods for marine glycoenzyme. We detect and quantify glycoenzymes in a variety of sample matrices using cutting-edge analytical techniques, including immunoassays, spectroscopy, and microscopy. This facilitates comprehensive analysis and validation of enzyme activity in complex biological samples or engineered systems.

• Fluorescent-based Marine Glycoenzyme Detection Service
• Affinity Glycan Labeling-based Marine Glycoenzyme Detection Service
• Chemoselective Reaction-based Marine Glycoenzyme Detection Service
• Metabolic Glycan Labeling (MGL)-based Marine Glycoenzyme Detection Service
• Chemoenzymatic Glycan Labeling (CeGL)-based Marine Glycoenzyme Detection Service
• Mass Spectrometry-based Marine Glycoenzyme Detection Service

Publication

Technology: Biochemical characterization of marine glycoenzyme

Journal: Applied and Environmental Microbiology

IF: 4.4

Published: 2021

Results: In this study, the authors conducted a biochemical characterization of glycolipid glycosyltransferase (GTcp) extracted from the marine bacterium "Candidatus Pelagibacter sp." strain HTCC7211 in the SAR11 clan. Findings revealed that GTcp possesses multifunctional properties, enabling it to synthesize diverse glycoglycerolipids using UDP-glucose, UDP-galactose, or UDP-glucuronic acid as sugar donors, and diacylglycerol (DAG) as an acceptor. When analyzing the enzyme's kinetic parameters, the authors discerned that Mg²⁺ significantly modifies GTcp's affinity with UDP-glucose, thus enhancing its catalytic efficiency.

Here are some of the results shown in this article:

Fig.2 UDP-sugar binding site of MshA and predicted UDP-sugar binding site of GT_cp. (Wei, et al., 2021)

Applications of Marine Glycoenzyme

• Marine glycoenzyme can be used to synthesize marine natural products. These enzymes catalyze the synthesis and modification of marine natural products to have higher activity and lower side effects.
• Marine glycoenzyme can be used to analyze the structure of sugar chains to help study the biological functions and mechanisms of action of sugars. Through the action of this enzyme, structural information of sugar chains can be obtained to better understand the role of sugars in life processes.
• Marine glycoenzyme can be used to study the metabolism and growth of marine microorganisms.
• Marine glycoenzyme can be used to study biological processes and interactions in marine ecosystems. This enzyme can be used to analyze carbohydrate metabolism and interactions between marine microorganisms in marine ecosystems.

Advantages of Us

• We offer custom development strategies to meet specific research needs, ensuring optimized performance and efficiency of marine glycoenzymes in a variety of applications.
• We offer innovative solutions through advanced R&D methods aimed at discovering novel marine glycoenzymes or enhancing existing ones to suit different biotechnological applications.
• We offer state-of-the-art analytical techniques and methods to precisely analyze, identify, and characterize marine glycoenzymes.
• We are committed to providing high-quality services within stipulated timeframes, ensuring reliable results for your project and timely delivery.

At CD BioGlyco, the one-stop marine glycoenzyme development service we provide covers the entire process from glycoenzyme design to detection. Please feel free to contact us if you are interested in our marine glycoenzyme development service! Our professional team will wholeheartedly provide you with the best service.