Empowering You with Pachymatismin Excellence!
CD BioGlyco possesses a series of advanced research tools for the production of various Marine Biomolecules, including Marine Carbohydrates, Marine Glycoproteins, Marine Lectins, Marine Glycosides, and Marine Glycolipids. Marine creatures prove to be valuable reservoirs of new bioactive substances due to their distinctive habitat compared to land-dwelling animals. Research on marine bioactive compounds predominantly centers on a diverse array of small molecular entities showcasing a wide spectrum of chemical configurations and medicinal characteristics. Within the vast realm of marine invertebrates, sponges (Porifera) and Cnidaria (Coelenterata) emerge as primary reservoirs of natural products in terms of both the quantity and complexity of extracted compounds. In this service, we are dedicated to the extraction and production of pachymatismin, a novel glycoprotein, from sponge bioresources.
Acquisition of sponge
High-quality sponges as raw material will be obtained and frozen at -20°C until further use.
Extraction
Thawed sponge samples will be homogenized in distilled water and centrifuged, and the supernatant will be dialyzed against distilled water followed by freeze-drying. This material serves as the starting material for purification.
Purification
- We conduct ammonium sulfate precipitation by adding solid ammonium sulfate at saturation to the crude extract. After standing, the mixture will be centrifuged. The resulting precipitate is suspended in a small volume of distilled water, filtered through a Millipore membrane, dialyzed against distilled water, and finally freeze-dried.
- Besides, we use multi-step gel filtration chromatography and fast protein liquid chromatography to further purify the pachymatismin.
Analysis
After the purification steps, the next phase involves conducting various analyses to characterize the purified compound. We will perform various analyses for key parameters, including:
- Identification: Anion exchange chromatography with amperometric detection will be used for the identification of the purified pachymatismin.
- Molecular weight: The molecular weight of pachymatismin will be determined by gel permeation chromatography (GPC).
- Protein assay: The Bradford assay will be used for measuring the concentrations of proteins in pachymatismin we produce, with bovine serum albumin as a standard.
- Amino acid analysis: We will hydrolyze the purified pachymatismin and then analyze it using a Waters Pica-Tag amino acid analysis system.
- Identification of sugars: We will analyze the acid hydrolysis of sugar using a high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
Publication Data
Journal: Marine Drugs
Published: 2017
IF: 5.4
Results: This literature review discusses the potential of compounds isolated from marine sponges as promising sources of anticancer agents. These compounds exhibit potential anticancer activity through various mechanisms, including cell-cycle arrest, anti-inflammatory activity, apoptosis, induction of ER stress, and interaction with multiple cancer-related targets. The compounds often affect multiple pathways, showcasing their multi-target nature to effectively combat the complexity of cancer. Some marine sponge derivatives demonstrate anticancer effects through particular and partially novel mechanisms, such as eribulin, which modulates microtubule dynamics differently from other antimicrotubular chemotherapy drugs. The authors mention that pachymatismin from Pachymatisma johnstonii inhibits the DU145 cell line through the target of microtubule depolymerization. In conclusion, this literature highlights the valuable research and clinical prospects of marine sponge derivatives as anticancer agents.
Applications
- Pachymatismin demonstrates potential applications as an antiproliferative agent, particularly in inhibiting cell growth in human non-small-cell bronchopulmonary carcinoma cells.
- Pachymatismin shows promise in biomedical research as a bioactive compound for studying the mechanisms of cancer cell proliferation.
- Pachymatismin may have future applications in drug development for lung cancer treatment, exploring its efficacy as a targeted therapy for NSCLC and other related malignancies.
Advantages
- We offer tailored production services for different types of hemocyanin to meet the specific requirements of clients.
- Our research team provides appropriate advice on selecting the most suitable hemocyanin type to ensure optimal choices for production.
- Our researchers utilize various methods to perform rigorous quality control tests and analyses to ensure the high quality of the produced hemocyanin.
Frequently Asked Questions
Can we proceed with subsequent activity assays on the produced pachymatismin?
Sure, our research team provides a comprehensive assessment of Biological Activities.
If the produced substance exhibits activity, can we perform structural modifications on it?
We provide extensive services for Modifying various marine carbohydrates by using various methodologies, expanding their applications, and supporting clients in their tissue engineering research endeavors.
With advanced biotechnological expertise and production tools, CD BioGlyco can efficiently purify and produce pachymatismin with high quality and high purity. Through our service, clients will obtain high-quality pachymatismin samples for further biological activity research and drug development. If you want to explore the potential of pachymatismin in the pharmaceutical field, please feel free to contact us! We are ready to provide reliable production solutions to our clients.
Reference
- Calcabrini, C.; et al. Marine sponge natural products with anticancer potential: an updated review. Marine Drugs. 2017, 15: 310.
