Marine Carbohydrate Characterization Service at CD BioGlyco
The ocean covers more than 70% of the world's surface and harbors a diverse range of living organisms. Carbohydrates obtained from marine algae, marine microorganisms, and marine animals usually have a wide range of biological activities, such as antioxidant, immune regulation, etc., and have huge application potential in the medical field. CD BioGlyco has specially established a marine biomolecule research team and has been concentrating on research in the field of marine sugar for many years. We provide clients with Extraction, characterization, and Modification services of marine biomolecules and application development services of marine biomolecules, such as Drug Delivery, Vaccine Development, Biomarker Development, etc.
Various substances have been extracted and characterized from marine organisms, such as chitin and chitosan from crustacean shells, carrageenan (CG) from red seaweed, alginate from brown seaweed, and exopolysaccharides (EPS) from marine bacteria, etc. To help clients explore marine biomolecules, we provide production and characterization services for carbohydrates derived from marine plants, marine animals, and marine microorganisms.
Our service process is as follows:
Marine Carbohydrate Extraction Services from Different Sources
Marine samples are preprocessed and carbohydrate components are obtained through different extraction techniques. Our commonly used extraction methods include traditional extraction methods (water extraction, acid, and alkali treatment extraction) as well as advanced extraction technologies such as microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), pressurized liquid extraction (PLE), and enzyme-assisted extraction (EAE). Extraction methods vary depending on the specific source and polysaccharide desired.
Marine Carbohydrate Purification Services from Different Sources
The crude extract obtained may contain impurities of different molecular weights, sulfates, contaminants, etc. We use different purification techniques to perform additional purification on the crude extract obtained, such as chromatography purification, precipitation purification, salting out purification, etc.
Marine Carbohydrate Characterization Services from Different Sources
To further determine the structure and function of the purified extract, we analyze the structure of polysaccharides using various techniques such as high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), mass spectrometry (MS), and Fourier transform infrared spectroscopy (FTIR), details as follows:
Fig.1 Marine carbohydrate characterization services from different sources. (CD BioGlyco)
Primary Structure Analysis of Marine Carbohydrate
- Determination of total sugar content: We use the phenol-sulfuric acid method, anthrone-sulfuric acid method, 3,5-dinitrosalicylic acid (DNS) colorimetric method, lichenol-hydrochloric acid method, and titration method to determine the total sugar content of polysaccharides.
- Monosaccharide composition and proportion: The monosaccharide composition analysis of polysaccharides is generally carried out first by acid hydrolysis to completely break the glycosidic bonds of the polysaccharide, and then through neutralization, filtration, derivatization, and other treatments and then measure using chemical and instrumental analysis techniques. Our commonly used measurement methods include but are not limited to gas chromatography (GC), HPLC, paper chromatography (PC), thin layer chromatography (TLC), high-performance capillary electrophoresis (HPCE), etc.
- Molecular weight size and distribution: The molecular weight of polysaccharides is the basis for studying the structural characteristics of polysaccharides and is also one of the important indicators for quality control. Polysaccharides from different sources have different molecular weight distribution ranges, which are commonly expressed by weight average molecular weight, number average molecular weight, and viscosity average molecular weight. Our commonly used measurement methods include gel permeation chromatography (GPC), high-performance gel permeation chromatography (HPGPC), dynamic and static light scattering, viscometry, etc.
- Glycosidic bond types and connection methods: A glycosidic bond is a specific type of chemical bond that connects the aglycone and the sugar group or the sugar group and the sugar group in the glycoside molecule. Commonly used methods to determine the type of glycosidic bond include infrared spectroscopy (IR), NMR, gas chromatography-MS (GC-MS), etc. We also combine chemical analysis methods to determine the glycosidic bond connection site, connection mode, sugar ring form of glycoside, and the proportion of different glycosidic bonds.
Advanced Structural Analysis of Marine Carbohydrate
Studying the spatial structure of polysaccharides not only helps to elucidate the structure-activity relationship but also completes the quality control of polysaccharides. The spatial structure of marine polysaccharides is very complex, and its spatial structure will be different depending on the extraction method, extraction site, or extraction source. The main methods for analyzing the advanced structure of polysaccharides include atomic force microscopy (AFM), X-ray diffraction (XRD), and circular dichroism spectroscopy (CD). At the same time, we also use computers to simulate the spatial conformation of polysaccharides to construct the three-dimensional structure of polysaccharides.
Here, we provide marine carbohydrate characterization services including but not limited to:
Polysaccharides derived from marine plants are widely used in the development of new drugs and nutritional foods. We provide clients with polysaccharide characterization services derived from various marine plants, including but not limited to:
Chitin and chitosan from crustaceans are representative marine polysaccharides. With advanced characterization technology, we provide clients with high-quality marine polysaccharide characterization services derived from animal origin, including but not limited to:
Carbohydrate polymers from marine bacteria exhibit enormous structural and functional diversity. We offer our clients characterization services for a wide range of marine polysaccharides of microbial origin, including but not limited to:
We use a variety of technologies to help clients analyze the structure of marine carbohydrates from different sources to help them research the use of marine carbohydrates in medicine and other fields. These technologies include but are not limited to:
- GC
- XRD
- TGA
- CHNS Elemental Analyzer
Publication
Technology: Extraction, purification, and characterization of polysaccharides from marine algae
Journal: Process Biochemistry
IF: 4.885
Published: 2018
Results: The author used MAE technology to extract polysaccharides from the seaweed Gracilaria lemaneiformis, and continuously optimized the extraction conditions to increase the polysaccharide yield (%) of crude polysaccharides. The polysaccharide extraction rate under optimal MAE conditions was 34.8%. The authors also purified the extracted crude polysaccharides by an aqueous two-phase system (ATPS) to obtain three fractions (GLP1, GLP2, GLP3), which were mainly composed of galactose. Among them, GLP-3 has the highest anti-tumor activity.
Fig.2 Extraction, purification, and characterization of polysaccharides from Gracilaria lemaneiformis. (Shi, et al., 2018)
Highlights
- We use traditional extraction methods (water extraction, acid, and alkali treatment extraction) and advanced extraction technologies (MAE, UAE, PLE, and EAE) to provide clients with polysaccharide extraction services from different marine biological sources.
- Purification techniques such as chromatographic purification, precipitation purification, and salting out purification are used to separate different fractions from the crude extract.
- Select the most appropriate characterization technique based on the type of polysaccharide or use a combination of methods to characterize the compound.
At CD BioGlyco, our professional marine biomolecule research team is committed to helping clients solve all the problems they encounter in marine biomolecule research. If you are looking for a partner in marine life research, please do not hesitate to contact us, we will be your best helper.
Reference
- Shi, F.; et al. Extraction, purification, and characterization of polysaccharides from marine algae Gracilaria lemaneiformis with anti-tumor activity. Process Biochemistry. 2018, 73: 197-203.
