Unlocking The Potential of Julianin with Our Manufacture Service!
In our research for glycobiology, CD BioGlyco unravels the vast potential harbored by the ocean utilizing its abundant biodiversity and sustainable resources to produce valuable Biomolecules. In this service, we produce a substance called julianin, which is found in the Sea hare’s water-soluble fraction of fetid secretion. Sea hares, belonging to the subclass Opisthobranchia within the realm of marine gastropods, exhibit a reduction in their shell, which becomes thin and transparent, ensconced by the mantle. To date, an array of biologically potent compounds, such as toxins and antibiotics, have been distilled from sea hares, predominantly from their digestive gland, and are conjectured to participate in the chemical defense mechanism of the animals against prospective predators. Sea hares are notorious for their propensity to discharge purple ink from the purple gland and a secretion from the opaline gland when perturbed.
We utilize this secretion to produce julianin through the following steps.
Collection of secretions of Aplysia juliana
Julianin will be extracted from specimens of A. juliana, which secretes a white and fetid substance from a gland. This malodorous secretion is pipetted out after the creatures are agitated. We will scrape off the bodily surface secretions using a spatula. Each secretion will be centrifuged and the resulting supernatant is then frozen for later use.
Extraction
We will extract the fetid secretion using n-hexane and then lyophilize the aqueous layer.
Purification
- The resulting substance is dissolved in distilled water and dialyzed against phosphate buffer.
- The retentate is concentrated through ultrafiltration using a membrane, followed by fractionation on a column using phosphate buffer.
- Subsequently, perform the ultra-filtered process and further process through fast protein liquid chromatography (FPLC) columns to purify the target product.
Characterization
Liquid chromatography-mass spectrometry (LC-MS) is for discerning and quantitatively delineating the realm of julianin. By this technology, we provide accurate empirical intel regarding the locale and extent of glycosylation sites, alongside the constitution, configuration, and dispersion of glycoforms.
Publication Data
Journal: Marine drugs
Published: 2020
IF: 5.4
Results: In this article, the authors discuss the significant diversity in structure and bioactivity of natural compounds found in heterobranch mollusks. These compounds from approximately 400 species of heterobranch molluscs, are crucial for snails' self-protection mechanisms and potentially valuable for human pharmacological purposes. In terms of pharmacology, these compounds exhibit cytotoxic and antitumoral properties, as well as antibiotic, antiparasitic, antiviral, and anti-inflammatory activities, with potential effects against neurodegenerative diseases. Cytotoxicity and anticancer effects are the most studied pharmacological activities, followed by antibiotic properties. The study mentions that the purple secretion from the sea hare A. juliana includes julianin-S, an antibacterial peptide proposed to safeguard its egg masses against microbial infections, alongside several unsaturated fatty acids. Heterobranch mollusks are particularly intriguing for their implications in marine natural product exploration, offering fertile ground for further research in chemical ecology and biotechnological applications.
Applications
- Julianin can serve as a potential drug candidate for investigating its pharmacological activities and drug interactions.
- Julianin can be used as a natural drug extract for pharmaceutical development and medical research.
- Julianin can also be applied as a potential antibacterial agent in food preservation research.
Advantages
- We possess deep expertise and technology to research and develop bioactive molecules from marine resources.
- Our research team utilizes advanced biotechnology and analytical techniques to extract, purify, and analyze target compounds, ensuring product quality and efficacy.
- Our researchers have rich experience and an excellent reputation in the biopharmaceutical field, providing reliable products, technical support, and solutions for clients and partners to establish long-term partnerships.
Frequently Asked Questions
Which marine resources can CD BioGlyco utilize for production?
- Marine Plant
- Marine Animal
- Marine Microbial
Can CD BioGlyco conduct activity analysis on the produced active substances?
We have the ability to analyze various bioactivities, including:
CD BioGlyco offers a comprehensive solution for producing, purifying, and analyzing glycoprotein found in marine organisms. This provision capitalizes on the organization's proficiency in glycobiology and marine biotechnology to furnish top-tier julianin for varied medical contexts. If you are interested in our dependable and inventive approach to exploiting marine assets for pharmaceutical exploration and natural medicine evolution, please feel free to contact us!
Reference
- Avila, C.; Angulo-Preckler, C. Bioactive compounds from marine heterobranchs. Marine drugs. 2020, 18(12): 657.
