Marine Microbial Polysaccharide Characterization Service

Marine Microbial Polysaccharide Characterization Service at CD BioGlyco

CD BioGlyco offers a wide range of Characterization Services for many types of marine carbohydrates including Plant Polysaccharides, Animal Polysaccharides, and microbial polysaccharides by combining various Characterization Techniques such as chromatography. Marine microbial polysaccharides have good bioactivity and also exhibit physical and chemical properties such as low toxicity, biocompatibility, biodegradability, etc., which have attracted much attention in the fields of biomedicine, food, cosmetics, etc. Comprehensive characterization of marine microbial polysaccharides is essential for their applied research. We provide a high-quality marine microbial polysaccharide characterization service to support our clients' related research.

• Characterization techniques related to marine microbial polysaccharides at CD BioGlyco:
  • Chromatography: High-performance liquid chromatography (HPLC), gas chromatography (GC)
  • Mass spectrometry (MS)
  • Nuclear magnetic resonance (NMR)
  • Fourier-transform infrared spectroscopy (FTIR)
  • Other characterization techniques: Scanning electron microscope (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), etc.
• Characterization project for marine microbial polysaccharides at CD BioGlyco:
  • Determination of polysaccharide content
  • Chemical composition analysis: Glucuronic acid content, protein content, organic content, etc.
  • Determination of molecular weight distribution
  • Structural identification: Order of monosaccharide residues, glycosidic bond position configuration, sugar ring configuration, sugar ring size, etc.
  • Polysaccharide NMR scanning analysis
  • Polysaccharide FTIP analysis
  • Advanced structure analysis of polysaccharides: thermal stability, surface structure, crystal structure, etc.

Our marine microbial polysaccharide characterization services are aimed at the following:

Fig.1 Marine microbial polysaccharide characterization service. (CD BioGlyco)

Publication Data

Technology: TGA, FTIR, GC

Journal: Marine Drugs

IF: 6.085

Published: 2021

Results: In this study, the extracellular polysaccharides of six Alteromonas strains from different marine environments in French Polynesia atolls were extracted and characterized. The results showed that the extracellular polysaccharides of Alteromonas strains are heteropolysaccharides composed of different monomers (uronic acids and neutral monosaccharides such as mannose, glucose, rhamnose, etc.). They have good thermal stability, gel-forming ability, and rheological properties, and have potential applications in drug delivery and tissue engineering.

Fig.2 TGA curves of extracellular polysaccharides produced by six Alteromonas strains. (Concórdio-Reis, et al., 2021)

Frequently Asked Questions

What are the characterization methods for microbial polysaccharides?

There are more characterization methods used for microbial polysaccharides.

• NMR: More detailed information about the molecular structure of microbial polysaccharides, including monosaccharide composition and type of glycosidic bonds, is obtained by NMR.
• HPLC: It separates different polysaccharide fractions by using different types of columns and is a commonly used technique for analyzing microbial polysaccharides, especially for separation and quantitative analysis.
• MS: MS analysis, especially in combination with liquid or gas chromatography, is widely used for the structural characterization of microbial polysaccharides. Detailed information on the composition of microbial polysaccharide sugar units, molecular mass, and linkage order is obtained by MS.
• FTIR: FTIR is used to identify functional groups in microbial polysaccharides, such as hydroxyl and aldehyde groups, which are particularly useful for analyzing the chemical structure.
• Other analytical techniques: TGA, SEM, XRD, etc.

Each of these methods has its advantages and limitations. A suitable method needs to be selected according to the nature of microbial polysaccharides and the purpose of the study. The purity and complexity of the samples as well as the level of detail of the information required also need to be taken into account in the experimental design.

How do you know the branched, terminal, side, and main chains by partial acid hydrolysis of polysaccharides?

Determining the branched, terminal, side, and main chain structure of polysaccharides by acid hydrolysis alone is relatively limited. Acid hydrolysis is primarily used to break down polysaccharides into smaller fragments and needs to be combined with the use of more advanced analytical techniques. For example, NMR can provide detailed information on the arrangement and linkage of sugar residues, while MS can provide information on molecular weight and composition.

Applications

• Marine microbial polysaccharide characterization is used to study and analyze the bioactive strength of microbial polysaccharides.
• Marine microbial polysaccharide characterization is used for the development of biomaterials based on microbial polysaccharides.
• Marine microbial polysaccharide characterization is used to study and optimize the extraction process of microbial polysaccharides.

Advantages of Us

• Marine microbial polysaccharide characterization is highly efficient and has a short project delivery cycle.
• We provide clients with satisfactory results by comprehensively designing programs and analyses according to their needs.
• We provide strong, thoughtful, and timely pre-sales and after-sales service support to answer any questions that arise.

At CD BioGlyco, our deep scientific expertise and experience in polysaccharides allow us to provide high-quality marine microbial polysaccharide characterization services. Welcome to contact us to learn more about marine microbial polysaccharide characterization if this is of interest to you or if you need it.