Marine Glycosidase Analysis Service

Marine glycosidase, a subtype of hydrolytic enzymes, plays an instrumental role in catalyzing the hydrolysis of glycosidic bonds in complex sugars. Being prevalent in marine ecosystems, these remarkable enzymes are critical in decomposing organic material, contributing to the carbon cycle.

Fig.1 LC-MS/MS-based glycosidase assays. (Skrinjar, et al., 2018)

CD BioGlyco specializes in Marine Glycoenzyme Development Services, which include Marine Glycosyltransferase Analysis Service and marine glycosidase analysis service. Our marine glycosidase analysis service aims to provide clients with an in-depth understanding and professional insights into marine glycosidases to comprehensively reveal the properties and functions of these important biomolecules. The analysis of marine glycosidase comprises their expression and purification, sequence and phylogenetic analysis, crystal structure determination, and the enzyme activity assay.

Fig.2 Marine glycosidase analysis service. (CD BioGlyco)

Isolation and Purification of Marine Glycosidase: We use advanced protein separation technologies, such as chromatographic separation, gel electrophoresis, etc., to obtain high-purity marine glycosidases. In addition, we use mass spectrometry-based technology to identify glycosidase, including protein sequence analysis and identification of post-translational modifications.

Sequence and Phylogenetic Analysis of Marine Glycosidase: We provide comprehensive sequence and phylogenetic analysis of marine glycosidase to understand the evolutionary relationships among organisms and to predict the properties of a new or altered gene. Our service includes gene sequencing, sequence alignment, phylogenetic tree construction, and sequence variation analysis.

We also provide glycosidase structure elucidation and enzyme activity detection services. We use X-ray crystallography technology to analyze the three-dimensional structure of marine glycosidase, which is beneficial to an in-depth understanding of its active center and catalytic mechanism. Marine glycosidase activity is determined by precisely measuring the rate of enzyme-catalyzed reactions. At the same time, we also conduct in-depth research and analysis on the optimal substrate, optimal reaction conditions, and other characteristics.

Publication

Technology: Biochemical analysis of marine glycoside hydrolase

Journal: Applied and Environmental Microbiology

IF: 4.4

Published: 2022

Results: The authors reported a new glycoside hydrolase family 2 (GH2), named AqGalA, isolated from a marine alga-associated Bacteroidetes organism, notable for its broad substrate specificity. The biochemical analysis showed that AqGalA efficiently broke down β-galacturonide, β-glucuronide, and β-galactopyranoside through retaining mechanisms. The structure of AqGalA in complex with galacturonic acid (GalA) was ascertained by crystallography, and subsequent mutagenesis helped determine that the charge traits at the uronate-binding subsites played a role in determining substrate selectivity for uronide hydrolysis. The authors proposed that the conformational flexibility of the AqGalA active-site pocket was a crucial factor for the broad substrate enzyme selectivity.

Here are some of the results shown in this article:

Fig.3 Phylogenetic and structural analyses of enzymes. (Yang, et al., 2022)

Applications of Marine Glycosidase Analysis

• Analysis of marine glycosidase provides an in-depth understanding of the activity, substrate specificity, and other characteristics of marine glycosidase, providing an important basis for the discovery of new marine drug candidates.
• Analysis of marine glycosidase provides an in-depth understanding of the distribution, function, and evolution of marine glycosidase in marine ecosystems, providing new perspectives and tools for marine ecological research.
• Analysis of marine glycosidase helps to study the application potential of different types of marine glycosidase in biofuel production and provides support for the development of efficient biofuel production technology.
• Analysis of marine glycosidase helps to study the application potential of different types of marine glycosidases in pollution control and provides support for the development of environmentally friendly marine pollution control technology.

At CD BioGlyco, our meticulous and data-driven analysis assists clients in advancing fundamental research on marine glycosidase. All services are carried out by a team of experienced scientists to ensure the quality and accuracy of the results. Please feel free to contact us if you are interested in our marine glycosidase analysis services!