Explore Cutting-edge Crambescidin Production at CD BioGlyco
CD BioGlyco has many years of experience in the scalable production of Marine Biomolecules, including Marine Carbohydrates, Marine Glycoproteins, Marine Lectins, Marine Glycosides, and Marine Glycolipids. In this service, CD BioGlyco ensures high purity and yield of crambescidins which are crucial marine glycoproteins from sponges for pharmaceutical and research applications. Marine sponges (phylum Porifera) stand as a paramount reservoir of novel bioactive compounds among marine organisms, consistently yielding over 200 newly documented compounds annually. These organisms synthesize a diverse array of secondary metabolites primarily for defensive purposes, safeguarding against threats posed by competitors, predators, and pathogens. Moreover, sponges often harbor microbial symbionts, recognized as pivotal contributors of bioactive agents.
Produce crambescidin by extraction
We mainly explore the extraction methods of the bioactive compounds derived from the various sponges, leading to the identification of a rich spectrum of crambescidin-type guanidine alkaloids. Our researchers undertake comprehensive efforts encompassing isolation, purification, structural elucidation, purity determination, and biological activity assay of these crambescidins.
- We will select suitable sponges and use organic solvents to extract crambescidin several times. Besides, combine the crude extracts and dry them under reduced pressure, resulting in an oily extract.
- Chromatography and high-performance liquid chromatography (HPLC) are utilized for the purification of crambescidin.
- We will use the nuclear magnetic resonance (NMR) technique in quality control and research for determining the structure and purity.
- Our Bioactivity Analysis will be performed to study the biological activities of crambescidins derived from diverse marine sources.
Produce crambescidin by chemical synthesis
Our research team is very professional in organic synthesis, therefore we provide the total synthesis, isolation, purity, and structural characterization of the crambescidin and its derivatives.
Publication Data
Technology: NMR, Time-of-flight mass spectrometer in positive electrospray ionization, Semi-preparative HPLC, Cell culture, Cell viability assay, Cell cycle analysis, Western blot analysis, Annexin-V-PT-binding assay, Immunofluorescence, Statistical analysis
Journal: Marine drugs
Published: 2018
IF: 5.4
Results: In this study, extracts from twenty marine sponges collected off the coast of Western Australia were screened for cytotoxic activity against triple-negative breast cancer (TNBC) cells. A highly active extract from the sponge Monanchora viridis was selected for bioactivity-guided fractionation. Through various purification processes, authors successfully isolated a potent cytotoxic compound, identified as crambescidin 800. Crambescidin 800 demonstrated significant cytotoxic effects across a range of breast cancer cell lines, with TNBC and luminal models showing the highest sensitivity. Crambescidin 800 decreased cell viability and caused cell cycle arrest at the G2/M phase in T11 and SUM159PT cells. Moreover, crambescidin 800 lowered the expression of proliferation-associated CDKs like cyclin D1, CDK4, and CDK6, while upregulating CDK inhibitors such as p21 at the protein level. Crucially, crambescidin 800 inhibited the phosphorylation of the Akt/mTOR, MAPK, and NF-κB pathways, which are implicated in tumor relapse and metastasis, leading to programmed cell death in T11 and SUM159PT TNBC cells. Overall, their findings suggest that crambescidin 800 has the potential to target multiple signaling pathways in TNBC cells, which are involved in cell survival and resistance to chemotherapy. Further research is needed to determine whether crambescidin 800 exhibits in vivo activity against TNBC, potentially paving the way for new anticancer therapies.
Applications
- Crambescidin can be used as a potential tool for its ability to inhibit cell migration by altering cytoskeletal dynamics, reducing actin expression, and inhibiting focal adhesion formation.
- Crambescidin is useful for studying malaria biology and exploring potential treatments for its activity against malaria parasites and its cytotoxic effects. It could also serve as a tool in broader biomedical research focused on understanding cellular mechanisms and drug development.
- Crambescidin is utilized as a candidate for further drug development efforts against cancer cells.
Advantages
- Our research team utilizes advanced extraction methods, including multiple organic solvent extractions, to ensure comprehensive retrieval of crambescidins from selected sponges, maximizing yield and purity.
- Our researchers have a good grip of advanced analytical techniques such as NMR to conduct thorough structural elucidation and purity determination of crambescidins.
- We offer tailored solutions to meet specific client needs, whether for large-scale production or specialized purification requirements, providing flexibility and personalized service.
Frequently Asked Questions
What are the biological activities of crambescidin?
- Antimalarial activity
- Antiviral activity
- Anti-neoplastic activity
- Antifungal activity
What types of crambescidin can we produce?
- Crambescidin 345
- Crambescidin 359
- Crambescidin 361
- Crambescidin 373
- Crambescidin 431
- Crambescidin 657
- Crambescidin 673
- Crambescidin 786
- Crambescidin 800
- Crambescidin 816
- Crambescidin 818
- Crambescidin 826
CD BioGlyco possesses a professional research team to deliver customized solutions to meet the specific needs of clients, whether it’s large-scale production or tailored purification requirements. By using advanced extraction and purification techniques, we assist pharmaceutical companies, research institutions, and biotech firms seeking reliable, bulk-scale production of bioactive marine compounds for drug discovery and development purposes. If you would like to access high-quality compounds for your research and development projects aimed at exploring the therapeutic potential of these marine-derived molecules, please feel free to contact us.
Reference
- Shrestha, S., et al. Crambescidin 800, isolated from the marine sponge Monanchora viridis, induces cell cycle arrest and apoptosis in triple-negative breast cancer cells. Marine drugs. 2018, 16(2): 53.
