Maximize Marine Research Potential with Our Comprehensive Extracellular Polysaccharide (EPS) Characterization
At CD BioGlyco, we pride ourselves on offering comprehensive Marine Carbohydrate Characterization Services, designed to delve into the complex world of marine-derived polysaccharides. Within this flagship service, our specialized Marine Microbial Polysaccharide Characterization Services stand out by focusing on the unique polymers sourced from marine microorganisms. A key subset of this service is our EPS characterization service, which zeroes in on the EPS produced by marine microbes.
Our EPS characterization service plays a critical role by providing detailed analysis of EPS, which is vital for microbial survival and has significant biotechnological applications. We employ state-of-the-art analytical techniques to decode the complex structures and diverse functions of these biopolymers. Through this service, clients can gain insights into the molecular composition, functional properties, and potential industrial applications of EPS.
Collection and Extraction
- Washing and filtration: We remove any debris and non-polysaccharide material from raw marine samples.
- Lyophilization or cryopreservation: We use this method to maintain sample integrity or for long-term storage.
- Hot water extraction: We use this method for water-soluble polysaccharides.
- Acid/base extraction: We use this method to release polysaccharides bound to other cellular components.
- Solvent precipitation: We typically use ethanol or acetone precipitation to separate polysaccharides from other soluble materials.
Purification
- Dialysis and ultrafiltration: We use this method to remove low molecular weight impurities.
- Chromatographic techniques: We use chromatographic techniques such as ion exchange chromatography, gel permeation chromatography (GPC), and affinity chromatography to separate and purify specific polysaccharide fractions.
Physicochemical and Functional Property Characterization
- Monosaccharide composition analysis: We use high-performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS) to identify the constituent sugars after acid hydrolysis.
- Molecular weight determination: We use techniques such as GPC and multi-angle light scattering (MALS) for molecular weight determination.
- Fourier transform infrared spectroscopy (FTIR): We use FTIR to identify characteristic functional groups.
- Nuclear magnetic resonance (NMR) spectroscopy: This technique provides detailed structural information, including glycosidic bonds and anomeric configuration.
- Rheological studies: We use this method to evaluate viscosity, gelation characteristics, and mechanical stability.
- Thermal analysis: We use differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) for thermal stability and decomposition characteristics.
- Emulsifying and film-forming ability: We use this method to evaluate potential applications in food, pharmaceuticals, and cosmetics.
- Antioxidant activity: We evaluate antioxidant activity using assays such as DPPH radical scavenging and reducing power assays.
Publication
Technology: Extraction and characterization of marine EPS
Journal: Marine drugs
IF: 6.085
Published: 2021
Results: The authors selected six Alteromonas strains from various marine environments in the atolls of French Polynesia for EPS extraction. The EPS was identified as heteropolysaccharides comprising various monomers, with neutral monosaccharides constituting up to 45.5 mol% of the compositions. Thermal analysis revealed that these EPS had degradation temperatures exceeding 260°C and exhibited high char yields ranging from 32% to 53%. Investigations into the functional properties of the EPS indicated their ability to generate viscous aqueous solutions with shear-thinning behavior and to form robust gels in two different manners.
Fig.1 FTIR spectra of EPS A–F produced by Alteromonas strains isolated from French Polynesia. (Concórdio-Reis, et al., 2021)
Applications of Marine EPS
- EPS can be utilized in the construction of hydrogels for biocompatible applications, providing support for cell growth and tissue engineering.
- EPS can be applied in formulations designed to neutralize free radicals in various biomedical products.
- EPS can enhance the performance of wound dressings by maintaining a moist environment, promoting faster recovery, and minimizing bacterial invasion.
Advantages
- We comprehensively analyze the fine structure of EPS, including their molecular weight, monosaccharide composition, and glycosidic bond type to provide detailed structural information for product application development.
- In addition to structural characteristics, we are committed to exploring the potential biological activities of EPS in immunomodulation, antibacterial, and antiviral aspects.
- We have many years of experience in the development and utilization of marine resources and continue to explore and characterize EPSs with unique structures and biological activities.
Frequently Asked Questions
What are marine EPSs and why are they important?
Marine EPSs are a class of complex carbohydrate macromolecules widely found in the marine environment, mainly secreted by algae, bacteria, and other microorganisms. These polysaccharides have unique biological activities and functions, such as antioxidant, anti-inflammatory, immunomodulatory, anticoagulant, and potential as drug carriers. Therefore, MEPs have extremely high application value and research significance in the fields of medicine, health products, cosmetics, and environmentally friendly materials.
What should I do if I have questions about the characterization results or need further analysis?
Clients can contact our project managers or technical experts directly. We will provide detailed explanations and develop supplementary experimental plans according to client needs. We always aim to satisfy our clients and are committed to providing comprehensive and professional service support.
At CD BioGlyco, our marine EPS characterization service is committed to unlocking the diverse potential of marine EPS through meticulous and advanced characterization techniques. Please feel free to contact us for more details if you are interested in our EPS characterization service!
Reference
- Concórdio-Reis, P.; et al. Characterization and biotechnological potential of extracellular polysaccharides synthesized by Alteromonas strains isolated from French Polynesia marine environments. Marine drugs. 2021, 19(9): 522.
