New Options for Innovative Research on Marine Glycopeptides Through Analysis
CD BioGlyco conducts in-depth research in Marine Glycoproteomics and introduces the latest technology and methods for analyzing glycoproteins. The analysis of marine glycopeptides helps to understand the relationship between their structure and functions and elucidates the regulation mechanism of glycosylation modification in marine organisms. Based on the perfect glycopeptide analysis process, we provide a professional marine glycopeptide analysis service. Our goal is to provide the optimal choice for marine glycopeptide analysis through customized solutions and help explore their physiological functions and other mysteries.
Marine Glycopeptide Analysis at CD BioGlyco
Marine glycopeptide analysis includes glycosylation sites, glycoform composition, quantitative analysis, etc. The high heterogeneity of glycosylation modifications, the diversity of peptide backbones and sugar chains, and the relative abundance of glycopeptides increase the difficulty of glycopeptide analysis. In order to achieve precise analysis, we have continuously researched, optimized, and developed a more complete, accurate, and reliable procedure for marine glycopeptide analysis. The specific experimental steps are as follows:
- Pre-treatment of samples: Marine biological samples are milled, lysed, etc., to release glycoproteins.
- Extraction and digestion of glycoproteins: Glycoproteins are extracted and purified by centrifugation, precipitation, etc., and digested into peptides by enzymatic digestion.
- Glycopeptide enrichment: Glycopeptide enrichment is required to increase the abundance of glycopeptides in a sample. We have a variety of glycopeptide enrichment techniques to improve the sensitivity of mass spectrometry (MS) analysis, including hydrophilic interaction liquid chromatography (HILIC), lectin affinity chromatography (LAC), enzyme chemistry, etc.
- Glycopeptide analysis: Glycosylation modification site distribution, glycotype composition, etc., are analyzed by liquid Chromatography with tandem mass spectrometry (LC-MS-MS).
- Bioinformatics analysis: We utilize advanced bioinformatics tools to accurately resolve marine glycopeptides. This process includes searching databases, recognizing and classifying glycoforms, glycopeptide abundance statistics, etc.
In addition to the detailed analysis of the results, we also provide attentive after-sales service and answer some of the questions that our clients encounter in their studies.
Applicable Samples
The compatibility of sample types used for marine glycopeptide analysis is broad and applicable to a wide range of complex biological samples.
- Plant tissue
- Animal tissues
- Cells
- Glycoproteins
- Plasma
- Other samples
Results Delivery
Typically, the following elements are included in our analysis reports:
- Analytical processes and methods
- Mass spectra
- Raw data
- Detailed marine glycopeptide analysis results
Publication Data
Technology: LC-MS/MS
Journal: Frontiers in Plant Science
IF: 6.627
Published: 2021
Results: In this study, glycoproteins and glycopeptides in Phaeodactylum tricornutum were analyzed using LC-MS/MS. The researchers extracted the glycoproteins from the algal cells after grinding and lysing them. Then they analyzed and classified the N-glycopeptide sequences through digestion and N-glycopeptide enrichment. A total of 863 different N-glycosylated peptides were eventually identified. Most of the identified N-glycopeptides were in the range of 9-23 amino acids in length. This study provides a method for the analysis of N-glycopeptides and the exploration of N-glycosylation pathways in P. tricornutum.
Fig.1 Characterization of N-glycosylation sites in P. tricornutum. (Xie, et al., 2021)
Applications
- Marine glycopeptide analysis is used to study the relationship between the structure and activity of marine glycopeptides.
- Marine glycopeptide analysis has important applications in the development of glycopeptide drugs.
- Marine glycopeptide analysis provides useful information for the development of biomaterials and tissue engineering research.
Advantages
- Our marine glycopeptide analysis process is well-established and accurate to support our clients' research and decision-making.
- Our method for the enrichment of marine glycopeptides has good specificity and high enrichment efficiency.
- Marine glycopeptides are analyzed by advanced technology with good reproducibility and accurate and reliable results.
Frequently Asked Questions
What are some strategies for glycopeptide enrichment?
Specific enrichment of glycopeptides from proteolytic samples is required to improve glycopeptide enrichment and the sensitivity of MS analysis. With the continuous development of research, various methods have been developed and improved to greatly enhance enrichment efficiency.
- HILIC: It is a common enrichment method for the separation and enrichment of glycopeptides through the difference between non-glycosylated and glycosylated peptides. The advantage of this method over other methods is that all types of glycopeptides are enriched without bias and their original structures are preserved.
- LAC: The enrichment of specific N- and O-glycopeptides is achieved by the binding properties of the lectin. The lectin is immobilized on magnetic beads or agarose, and the glycopeptides are separated and enriched by selectively recognizing sugars with specific structures.
- Enzyme chemistry: This method combines chemical and enzymatic reactions to recognize, capture, and release glycopeptides. It is also one of the commonly used methods for glycoproteomics.
How long does it take to analyze marine glycopeptides and can it be customized?
The time required for marine glycopeptides analysis varies depending on the complexity of the sample. Specific experimental steps may vary. After understanding the purpose of the analysis and the sample type of the client, we will customize the fitting experimental plan, and communicate.
CD BioGlyco has a team with extensive hands-on experience in marine glycoprotein analysis to take care of the development of marine glycopeptide analysis protocols and experiments. We pursue advanced technology to bring valuable assistance to our client's research. Welcome to contact us if you need to analyze marine glycoproteins or glycopeptides.
Reference
- Xie, X.; et al. Global profiling of N-glycoproteins and N-glycans in the diatom Phaeodactylum tricornutum. Frontiers in Plant Science. 2021, 12: 779307.
