Marine Glycosyltransferase Analysis Service

CD BioGlyco provides a comprehensive Marine Glycoenzyme Development Service, including the marine GT analysis service. Our marine glycosyltransferase (GT) analysis service aims to provide clients with an in-depth understanding and professional insights into marine GTs to comprehensively reveal the properties and functions of these important biomolecules.

Fig.1 Marine GT analysis service. (CD BioGlyco)

• Isolation and Purification of Marine GT: We utilize a variety of advanced methods for isolating this specialized enzyme from complex mixtures of marine organisms. To ensure efficient and effective extraction and purification, we employ a variety of methods including enzymatic digestion, cell disruption, centrifugation, precipitation, chromatography, and electrophoresis.
• Bioinformatic Analysis of Marine GT: Bioinformatics tools and software are used to analyze sequence data, predict structural motifs, and determine evolutionary relationships. This analysis helps infer the functional domain, substrate specificity, and evolutionary history of the enzyme.

Fig.2 Identification of key residues for diacylglycerol binding in the GT. (Wei, et al., 2021)

The structure analysis and enzyme activity analysis of marine GTs are crucial steps in understanding these enzymes' behavior and their role in biological processes. The structure of the enzyme provides detailed insights into its functionality, its interaction with substrates and inhibitors, and its evolutionary relationship with other enzymes. The enzyme activity assay, meanwhile, allows us to quantify the enzyme's catalytic capacity and its kinetics parameters, which is essential in the characterization of its physiological role. Therefore, we provide structural analysis and enzyme activity analysis for marine GT.

Publication

Technology: Phylogenetic analysis

Journal: Environmental microbiology

IF: 5.1

Published: 2016

Results: The authors conducted a genome-wide identification of macrolide GT genes within marine-derived Bacillus methylotrophicus B-9987, leading to the discovery of three such genes: bmmGT1-3. The function of bmmGT1, previously shown to contribute to the biosynthesis of trans-acyltransferase (AT) polyketides compounds macrolactins (MLNs) and Bacillaenes (BAEs), was already known. However, the authors investigated the roles of bmmGT2 and bmmGT3, revealing their capacity to biochemically catalyze glycosylation of MLNs and BAEs, albeit with different regioselectivity that resulted in four new MLNs analogs. Additionally, upon further genome mining, it was found that these three macrolide GT gene orthologs are regularly distributed within the subtilis- and cereus-clade Bacillus strains. Interestingly, bmmGT1 orthologs only appeared within the subtilis-clade Bacillus and were also located within Streptomyces strains' genomes, indicating a possible close phylogenetic relationship.

Here are some of the results shown in this article:

Fig.3 Phylogenetic analysis. (Liu, et al., 2016)

Applications of Marine Glycoenzyme Design Strategies

• An in-depth study of marine GTs helps scientists discover new drug targets and supports the development of new marine drugs.
• Marine GTs can be used to develop new marine biocatalysts and biomaterials for the production of high-value chemicals, biofuels, etc.
• Marine GTs can be used for biodegradation of marine pollutants and environmental remediation.
• The gene sequence, amino acid sequence, and structural information of marine GTs can be used to classify and identify marine organisms.

Advantages of Us

• We use advanced genetic engineering technology, X-ray crystallography analysis technology, bioinformatics methods, and chromatography technology to analyze marine GTs.
• We provide clients with a full range of marine GT analyses, including structure elucidation, activity analysis, and stability evaluation.
• We provide personalized service solutions based on client needs and specific conditions, including customized experimental design, data analysis, and service reports.
• We have an efficient operating mechanism and a professional service team that quickly responds to client needs.

At CD BioGlyco, we use cutting-edge technologies, bioinformatics tools, and analytical methods to reveal the complex functions and characteristics of marine GTs. These insights help to understand their potential applications in biosynthetic pathways, structure-activity relationships, and various fields. Please feel free to contact us if you are interested in our marine GT analysis services!