Empowering Cancer Research with High-Quality Luzonicosides from CD BioGlyco
CD BioGlyco provides high-quality and custom production services for luzonicosides, which are cyclic steroid glycosides derived from the starfish Echinaster luzonicus. Luzonicoside A and D exhibit strong anticancer properties, effectively inhibiting the proliferation, colony formation, and migration of human melanoma cells. The production process at CD BioGlyco includes the meticulous extraction of glycosides from the starfish, followed by advanced fractionation and purification techniques using chromatography. To ensure the highest quality, these compounds' structural characterization and quality control are carried out with nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and bioassays, guaranteeing their purity and bioactivity. This comprehensive approach highlights CD BioGlyco's commitment to delivering High-quality Marine Glycosides for research applications.
Fig.1 Structures of luzonicoside A (LuzA) (1) and luzonicoside D (LuzD) (2) isolated from the starfish E. luzonicus. (Malyarenko, et al., 2017)
Our detailed workflow ensures we deliver high-quality luzonicosides, particularly in the development of anticancer treatments. The use of advanced extraction, purification, and characterization techniques underscores the company's commitment to excellence in marine-derived bioactive compounds. Moreover, we also offer comprehensive Marine Biomolecule Production solutions for clients worldwide.
Collection
The process begins with the collection of the starfish E. luzonicus from their natural habitats. The collected starfish are cleaned and their tissues are prepared for extraction by homogenizing them into a uniform mixture.
Extraction
The homogenized starfish tissues are extracted using a suitable solvent system, typically involving organic solvents like methanol or ethanol.
Fractionation
- Initial fractionation: The crude extract is subjected to an initial fractionation process to separate the glycoside-rich fraction from other components.
- Advanced fractionation: The glycoside-rich fraction is further processed using more refined fractionation techniques such as column chromatography.
Purification
The concentrated fractions undergo purification through advanced chromatographic techniques.
Characterization
- NMR spectroscopy is employed to determine the molecular structure of the purified luzonicosides.
- MS is used to determine the molecular weight and verify the molecular formula of the luzonicosides, ensuring the correct identification of each compound.
Quality control
High-performance liquid chromatography (HPLC) is used to assess the purity of the isolated luzonicosides.
Publication
DOI: 10.3390/md15070227
Technology: Cytotoxicity assays, Cell proliferation assays, Cell cycle analysis
Journal: Marine Drugs
IF: 4.9
Published: 2017
Results: The study highlights the potent anticancer activity of luzonicosides A and D. These compounds significantly inhibit the proliferation, colony formation, and migration of human melanoma cells, specifically in the RPMI-7951 and SK-Mel-28 cell lines. Luzonicoside A demonstrated greater efficacy compared to luzonicoside D. The research further elucidated the molecular mechanisms underlying these effects, involving the regulation of cell cycle arrest and apoptosis. Key molecular targets include cleaved caspase-3, PARP, Survivin, Bcl-2, p21, and Cyclin D1, indicating a comprehensive modulation of cell death pathways and cell cycle control.
Applications
- Luzonicosides can be used to explore the molecular mechanisms of cancer cell inhibition, such as the regulation of apoptosis, cell cycle arrest, and the inhibition of metastasis-related pathways.
- Luzonicosides can be used in research helps identify specific molecular targets and pathways involved in cancer progression, offering insights into novel strategies.
- Luzonicosides are used in various preclinical models, including in vitro cell culture studies and in vivo animal models, to evaluate their efficacy and safety profile as potential therapeutic agents.
Advantages
- The purity and structural integrity of luzonicosides are confirmed using NMR spectroscopy and MS, providing researchers with reliable and consistent compounds.
- CD BioGlyco offers custom production services to meet the specific needs of clients, including different purity levels, quantities, and analytical services.
- CD BioGlyco's team of scientists and technicians bring extensive expertise in marine natural products, ensuring the production of high-quality compounds.
Frequently Asked Questions
Can CD BioGlyco customize luzonicoside production to specific research needs?
Yes, CD BioGlyco offers customized production services tailored to the specific needs of researchers and developers, including different purity levels, quantities, and additional analytical services.
What quality control measures are in place for luzonicoside production?
Quality control includes rigorous testing for purity using HPLC, structural verification with NMR and MS, and bioactivity assays to ensure the Luzonicosides meet high standards for research and therapeutic use.
At CD BioGlyco, our luzonicoside production service provides valuable tools for advancing cancer research, leveraging cutting-edge technology and scientific expertise to deliver top-quality marine glycosides. Please do not hesitate to contact us for more details about our service and feel free to get in touch for your solution.
References
- Malyarenko, O.S.; et al. The inhibitory activity of luzonicosides from the starfish Echinaster luzonicus against human melanoma cells. Marine Drugs. 2017, 15(7): 227.
- From Wikipedia: https://en.wikipedia.org/wiki/Echinaster_luzonicus#/media/File:Coeloplana_astericola_(Benthic_ctenophores)_on_Echniaster_luzonicus_(Seastar).jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/Liquid%E2%80%93liquid_extraction#/media/File:Funnels.jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/Column_chromatography#/media/File:Column_chromatography_sequence.png.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Agilent1200HPLC.jpg.
