Experience Unmatched Accuracy in Marine Polysaccharide Analysis with Our Cutting-edge Galactan Characterization Service
At CD BioGlyco, we are deeply involved in the field of marine biotechnology and are committed to providing cutting-edge and professional Marine Carbohydrate Characterization Services to global clients. Under this comprehensive service framework, we refine a number of special services to meet different scientific research and industry needs accurately. Among them, Marine Plant Polysaccharide Characterization Service as an important branch, focuses on the in-depth analysis of complex polysaccharides from marine plants. Going further, we are proud to offer galactan characterization service, focusing on the comprehensive characterization of galactan.
Galactan characterization service uses cutting-edge biochemical technology to conduct a comprehensive analysis of galactan from molecular structure to biological activity. We not only stop at the simple determination of its chemical composition, but also go deeper into the core aspects of its glycosidic bond type, molecular weight distribution, functional group characteristics, and activity evaluation.
Sample Extraction and Purification
- Selected raw materials: We select galactan-rich organisms from high-quality marine resources, such as seaweed and marine microorganisms to ensure the purity and activity of the raw materials.
- Optimized extraction process: We use advanced extraction techniques, such as ultrasonic-assisted extraction and enzymatic hydrolysis to maximize the retention of the biological activity and structural integrity of galactan.
- Purification steps: We remove impurities through multiple chromatography (such as ion exchange chromatography and gel filtration chromatography) and electrophoresis technology to obtain high-purity galactan samples.
Structural Characterization
- Molecular weight determination: We use gel permeation chromatography (GPC) combined with laser light scattering technology to accurately determine the molecular weight distribution of marine galactan.
- Monosaccharide composition analysis: We use high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS) technology to qualitatively and quantitatively analyze the monosaccharide composition of the hydrolyzed samples.
- Glycosidic bond connection type analysis: We use nuclear magnetic resonance (NMR) technology, especially one-dimensional (1H, 13C) and two-dimensional (COSY, HSQC, HMBC) NMR to analyze the connection mode and sequence of glycosidic bonds in detail.
- Advanced structural analysis: We combine circular dichroism (CD), X-ray diffraction (XRD), and other technologies to explore the advanced structural characteristics of marine galactan.
- Morphological observation: We observe the microscopic morphology of galactan, including particle size, shape, and surface structure by scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
Physicochemical Property Evaluation
- Viscoelastic analysis: We use a rheometer to evaluate the solution behavior of marine galactan to understand its application potential as a thickener and stabilizer in food, cosmetics, and other fields.
- Interaction study: We use isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and other technologies to study the interaction mechanism between marine galactan and other biomolecules, providing a theoretical basis for drug design.
- Thermal stability analysis: We use differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) to evaluate the stability and thermal degradation behavior of galactan at different temperatures.
Biological Activity Evaluation
- Antioxidant activity: We use a variety of in vitro antioxidant models to evaluate the antioxidant capacity of galactan.
- Immunomodulation: We use cell experiments to study the effects of galactans on the activation of immune cells and cytokine secretion.
- Other biological activities: We conduct various biological activity evaluations including antibacterial, antiviral, anti-inflammatory, and anti-tumor activity according to client needs.
Publication
Technology: Anticoagulant activity test
Journal: Anais da Academia Brasileira de Ciências
IF: 1.854
Published: 2023
Results: The authors aimed to characterize and evaluate the anticoagulant potential of sulfated polysaccharides (SP) extracted from the green algae Halimeda opuntia. Four SP-rich fractions: F23, F44, F60, and F75 were obtained through proteolytic digestion using papain, followed by ethanol precipitation. Agarose gel electrophoresis confirmed the presence of SP, revealing different populations within each fraction. Among these, the F44 fraction stood out, yielding 5% of the initial algae weight and demonstrating anticoagulant activity via the activated partial thromboplastin time assay, which measures the intrinsic/common coagulation pathway. Interestingly, further purification of F44 resulted in prothrombin time activity, alongside the enhanced activated partial thromboplastin time activity. The anticoagulant activity of P1F44 was found to be dependent on coagulation factors IIa and Xa, with a higher potency observed via factor IIa.
Fig.1 Agarose gel electrophoresis of ethanol-precipitated SP fractions (F23, F44, F60 and F75) obtained from H. opuntia. (Costa, et al., 2023)
Applications of Marine Galactan
- Food thickener and stabilizer: Marine galactan can be used as a thickener and stabilizer in the food industry due to its unique physicochemical properties, such as high viscosity and good water solubility.
- Biomaterial ingredient: Marine galactan can be used in the preparation of biomaterials due to its natural origin and good biocompatibility. It can be used as a matrix material and combined with other bioactive molecules to form a composite material with specific functions.
- Cosmetic additive: Marine galactan can be used as an important additive in the cosmetics industry due to its excellent moisturizing properties and skin affinity. Marine galactan also has antioxidant and anti-inflammatory effects, protecting the skin from damage by the external environment.
Advantages
- Our services cover multiple key properties of marine galactan, from molecular structure to physicochemical properties to biological activity evaluation, providing comprehensive and in-depth analysis reports.
- We have a team of senior industry experts who not only have superb technology but also have deep industry insights to provide clients with professional technical consultation and solution suggestions.
- We are constantly innovating technology and are committed to improving the efficiency and accuracy of the marine galactan characterization service.
Frequently Asked Questions
What are the characteristics of a marine galactan?
Marine galactan is a glycan extracted from marine algae with unique physical and chemical properties, such as high viscosity, good water solubility, and stability. Its molecular structure is complex and contains abundant sulfate groups and other functional groups. These characteristics make marine galactan have a wide range of application potential in food, cosmetics, biomaterials, and other fields. At the same time, its biological activities such as antioxidant and anti-inflammatory also provide a basis for further research and development.
What sample information or preparations do I need to provide to start this service?
In order to successfully start the marine galactan characterization service, you need to provide a sufficient amount of pure marine galactan samples and describe the source of the sample, extraction method, preliminary purity assessment, and other information as detailed as possible. In addition, if you have specific analytical needs or concerns, please let us know in advance so that we customize a personalized service plan. We guide you to complete the packaging and preservation of the sample before shipping to ensure that the sample is not damaged during transportation.
CD BioGlyco is committed to providing clients with comprehensive, accurate, and efficient marine polysaccharide characterization solutions through the marine galactan characterization service, helping clients achieve breakthrough progress in scientific research and product development. Please feel free to contact us for more details if you are interested in our marine galactan characterization service!
Reference
- Costa, B.B.; et al. Partial characterization and anticoagulant activity of sulfated galactan from the green seaweed Halimeda opuntia. Anais da Academia Brasileira de Ciências. 2023, 95(2): e20211002.
