Chondroitin Sulfate-based Drug-Delivery Vehicle Development Service

Equipped with world-leading marine glycobiology technology and professional scientific staff, CD BioGlyco provides high-targeting Marine Carbohydrate-based Drug-Delivery Vehicle Development services such as Alginate, Fucoidan, and Chitin. Chondroitin sulfate (CS) is a polysaccharide composed of D-gluconate acid and N-acetyl galactose linked by β-1,4. Specifically, CS recognizes folate receptors that are overexpressed on tumor cells with an effective tumor recognition function. Based on this, CD BioGlyco provides functionalized CS-based drug-delivery vehicle development services. Our researchers design and synthesize a series of CS-based drug-delivery vehicles such as polymer nanoparticles, and micelles, for target drug delivery to specific locations.

Fig.1 Flowchart of CS-based drug-delivery vehicle development services. (CD BioGlyco)

CS-based Drug-Delivery Vehicle Development Service at CD BioGlyco

At CD BioGlyco, we consider the solubility of drugs and the mode of administration, providing vehicle development services with adjustable sizes and a variety of morphology. Furthermore, we further provide evaluation services for CS-based drug-delivery vehicle self-assembly capacity, drug release behavior, tumor cell uptake, and cytotoxic effects.

Self-assemble Vehicle Service

• Synthesis Vehicle: Our development team provides self-assembly vehicle services by controlling molecular and solvent parameters. A CS is constructed as a water-soluble backbone material by Modification of hydrophobic molecules. Drug vehicles for redox-sensitive and nonsensitive amphipathic polymers are prepared by polymer self-assembly (polymer nanoparticles and micelles).
• Characterization Service: For synthetic polymers, we provide structural characterization services including nuclear magnetic resonance (NMR), transmission electron microscope (TEM), Fourier transform infrared spectrometer (FTIR), dynamic light scattering (DLS) analysis, etc.

In Vitro Drug Release

• Drug release study service: We evaluate the drug release behavior of the CS-based drug-delivery vehicle under different conditions by dialysis membrane analysis service. We determine the drug content in the release medium by high-performance liquid chromatography (HPLC).
• Cell uptake: We observe the qualitative and quantitative uptake of the synthetic vehicle by cells by fluorescence microscopy and flow cytometry, respectively.
• Other services: In addition, we provide cytotoxicity analysis service, immunofluorescence analysis service, reactive oxygen species analysis service, and live-dead cell viability assay analysis services.

In Vivo Targeting Efficacy

Our researchers establish a mouse model of disease by subcutaneous cell injection. Following drug administration, drug in vivo distribution is determined by in vivo imaging analysis. We provide in vivo bioactivity analysis services by calculating mouse tumor volume, relative tumor volume, and tumor growth inhibition rate.

Fig.2 Schematic illustration of CS-based drug-delivery vehicle synthesis and analysis. (CD BioGlyco)

Publication

Technology: NMR, Pyrene fluorescence probe, DLS

Journal: Acta Biomater

Published: 2022

IF: 9.7

Results: In this article, a strategy for designing prodrug nanoparticles based on CS is established. Nanoparticles achieve reduced photodynamic therapy (PDT)-mediated immunosuppression by interfering with the production of the Golgi apparatus and immunosuppressive factors. This strategy effectively intervened with PDT-induced immunosuppression and showed good antitumor efficacy of PDT in the b16f10-subcutaneous-bearing mouse model.

Fig.3 Expression of immunosuppressive cytokines in tumor tissues. (Li, et al., 2022)

Applications

• The CS-based drug-delivery vehicles are used as the drug vehicle of the small molecule hydrophobic drugs (paclitaxel, doxorubicin, camptothecin, etc.), and then play a role in increasing the drug dissolution, slow-release, and improving its stability.
• The CS-based drug-delivery vehicle is used as a non-viral-like gene-release vector. The hydrophobic functional groups on amphiphilic polysaccharides enhance cell endocytosis, reduce the adhesion of genes to serum ferritin, and greatly improve the rate of gene transfection.

Advantages of Us

• The hydrophilic backbone of the CS-based drug-delivery vehicle has functional groups (carboxyl and amino groups) that surface ligands, which makes it easier to achieve active targeting of tumors.
• Well-designed CS-based drug-delivery vehicle service we provide has the advantages of improving the solubility of low-soluble drugs, protecting the encapsulated drug, and targeting tumor delivery through receptor-mediated active targeting or enhanced permeability and retention (EPR) effects.
• The CS-based drug-delivery vehicle we provide responds to the reductive conditions of tumor cells and releases drugs quickly and thoroughly, thus improving drug efficacy.

CD BioGlyco provides marine carbohydrate-based drug-delivery Vehicle development services to our clients worldwide. Our professional service attitude and efficient service quality have been recognized by our clients. If you are interested in our service, please feel free to contact us.