Chitosan-based Tissue Scaffold Development Service

Chitosan-based Tissue Scaffold Development Service at CD BioGlyco

CD BioGlyco integrates the research and development, fabrication, and sales service of tissue scaffolds based on Marine Carbohydrates (Hyaluronic Acid, Alginate, Chitosan, Carrageenan, etc). Chitosan is a natural polymer extracted from crustaceans such as crabs and lobsters. It consists of two monomers, N-acetyl-D-glucosamine and β-(1→4)-linked D-glucosamine. We offer chitosan-based tissue scaffold development services for multiple tissue engineering.

Chitosan-based Tissue Scaffold Development Service

Chitosan has three important functional groups including an amino group, secondary hydroxyl groups, and primary hydroxyl groups. These functional groups readily generate intermolecular hydrogen bonds and are cross-linked with other polymer chains, so chitosan is used to make various types of composite scaffolds.

Fig.1 Schematic representation of applications of chitosan-based bioactive materials. (Islam, et al., 2020)

We use chitosan-based tissue scaffold preparation methods including gas foaming, particle leaching, freeze-drying, solvent casting, electrospinning, 3D printing/bioprinting, and others. Chitosan has osteoconductive, osteoinductivity, biodegradability, antimicrobial properties, and good drug delivery properties. The main factors we consider when fabricating chitosan scaffolds include molecular weight, degree of deacetylation, size, and structure of the scaffold, appropriate pore size and porosity, and proportion of chitosan. We develop tissue scaffolds for several applications, including bone tissue engineering, vascular tissue engineering, skin tissue engineering, and others.

Fig.2 Process of fabrication of chitosan-based tissue scaffolds. (CD BioGlyco)

Modification of Chitosan

Pure chitosan scaffolds are found to have some disadvantages, such as rapid degradation and poor mechanical properties. We enhance the activity of chitosan by copolymerizing it with polymers or modifying it by introducing groups to the chitosan side chain. The main methods of chemical modification are quaternization, carboxylation, sulfation, and phosphorylation. In addition to chemical modification, we also combine chitosan with other materials to improve its properties, including hyaluronic acid, gelatin, polyvinyl alcohol, and more.

Publication Data

Technology: Compression method

Journal: Carbohydrate Polymers

IF: 5.158

Published: 2017

Results: In this study, a new compression method was developed to prepare chitosan scaffolds based on the solubility and ionic strength of chitosan. The results of preparing scaffolds by this method showed an increase in the compression elasticity of the scaffolds and a decrease in porosity when the compression ratio was increased. This method does not require crosslinking agents. Also, it overcomes the problems of high viscosity and low solubility of polysaccharide solution with more advantages.

Fig.3 Confocal micrographs of human adipose-derived stem cells on chitosan scaffolds formed using different compression ratios. (Xu, et al., 2017)

Applications

• Chitosan-based tissue scaffolds are used to create bioactive membranes. These biofilms promote the growth of specific cells.
• Chitosan-based tissue scaffolds are used as drug delivery systems to guide tissue regeneration procedures.
• Chitosan-based tissue scaffolds are used as potential coating materials for orthopedic implants.

Highlights of Us

• The chitosan-based scaffolds developed by us exhibit physicochemical, mechanical, and biocompatibility properties that are adequate for our clients' research purposes.
• We use a variety of polysaccharide-based tissue scaffold preparation methods for chitosan-based tissue scaffolds, such as 3D printing, freeze-drying, and solvent casting.
• During the development of chitosan-based tissue scaffolds, we look for the appropriate porosity, pore size, mechanical strength, etc.

CD BioGlyco is experienced in tissue scaffold development. We are committed to meeting our clients' biomaterial development needs in various fields such as bone tissue engineering, skin regeneration, and periodontal tissue. Please feel free to contact us for more detailed information on tissue scaffold development. We look forward to supporting your tissue engineering research.