Precision Laminarin Characterization for Optimal Applications
Marine plants including seaweed and algae are rich sources of polysaccharides with unique structures and biological activity. Because they have potential in various industries such as medicines, cosmetics, and biotechnology, these marine plant polysaccharides have caused great interest in people. CD BioGlyco provides clients with comprehensive Marine Plant Polysaccharide Characterization with advanced technical means. Laminarin is a storage polysaccharide found in brown algae. It has attracted great attention due to its immune regulation, antioxidant characteristics, and potential anti-cancer effects.
Purity Determination of Laminarin
The purity of laminarin samples is critical to ensure their efficacy and safety in applications. We use advanced techniques to measure the purity of laminarin samples. High-performance liquid chromatography (HPLC) is used to separate, identify, and quantify the components in laminarin samples. It also distinguishes laminarin from any impurities or contaminants present in the sample. Gas chromatography-mass spectrometry (GC-MS) detects volatile and semi-volatile organic contaminants in laminarin samples by separating chemical mixtures and identifying individual components at low concentrations. Fourier transform infrared spectroscopy (FTIR) is used to identify functional groups and characterize chemical bonds, thereby verifying the presence and purity of laminarin in the sample.
Composition Analysis of Laminarin
Determine the composition of laminarin including identification and quantitative monosaccharides and other ingredients that constitute polysaccharides. We use HPLC or GC-MS to analyze the monosaccharides of laminarin after hydrolysis, which is essential for understanding the structural characteristics and potential biological activity of laminarin. UV-visible (UV-VIS) spectrum is used to evaluate the content of total carbohydrates. In some cases, it also identifies specific functional groups or impurities.
Structural Analysis of Laminarin
- Nuclear magnetic resonance (NMR): Determine the molecular structure and provide more detailed information about the connection between atoms.
- MS: Provide detailed information about oligosaccharides and structures generated by laminarin hydrolysis.
- FTIR: Analyze the vibration mode of the chemical bond to identify the characteristic functions and keys in polysaccharides.
Publication
Technology: Ultrasound-assisted extraction, Solid-liquid extraction, Matrix-assisted laser desorption ionization quadrupole time-of-flight-MS (MALDI-Q-TOF-MS)
Journal: Marine drugs
IF: 4.9
Published: 2015
Results: The authors used ultrasound-assisted extraction and solid-liquid extraction techniques to obtain crude laminarin extracts from Ascophyllum nodosum and Laminaria hyperborea. Then they used 10 kDa molecular weight cut-off dialysis to purify the crude extract to obtain laminarin, which was characterized by MALDI-Q-TOF-MS. The authors also determined the antioxidant and antibacterial activities of laminarin extracted from different sources. The results showed that both extracts showed high antioxidant activity against 2,2-diphenyl-1-trinitrophenylhydrazine (DPPH) and also had antibacterial activity, which has potential application in the preparation of antibacterial products.
Fig.1 MALDI-Q-TOF-MS spectra of Laminaria hyperborea extract. (Kadam, et al., 2015)
Applications
- Study the properties of laminarin such as moisturizing, anti-aging, and antioxidant activities for the development and optimization of cosmetic formulations.
- Understand the interaction of laminarin with other drugs and its potential as a drug delivery system due to its biocompatibility and biodegradability.
- Determine the detailed molecular structure of laminarin to help understand its efficiency of conversion to fermentable sugars and subsequent biofuel production.
- Characterize laminarin to develop natural biostimulants to improve crop yields and health without relying on chemical fertilizers.
Advantages of Us
- Our characterization services cover a wide range of laminarin analysis, ensuring a detailed understanding of its structural and functional properties. This is essential for academic research and industrial applications that require a precise understanding of polysaccharide properties.
- We use state-of-the-art analytical techniques such as HPLC, MS, and NMR to ensure accurate and comprehensive characterization. These advanced techniques provide detailed analysis of molecular weight, monosaccharide composition, glycosidic bonds, and structural conformation.
- We take stringent quality control measures to ensure the reliability and reproducibility of analytical results. This is essential to maintain consistency in your research or product development process.
- This service is applied to laminarin samples from a variety of marine algae. This flexibility enables researchers and industrial clients to analyze a variety of samples, facilitating comparative studies and quality assessments.
Frequently Asked Questions
Can you identify contaminants in laminarin samples?
Yes, an important aspect of laminarin characterization is the detection and identification of contaminants. We use HPLC, MS, and NMR to verify the purity of laminarin and identify any potential contaminants such as proteins and peptides, small molecule impurities, and residual solvents.
What types of samples can the laminarin characterization service be used for?
Our laminarin characterization services are suitable for a variety of sample types: 1) raw material samples such as algae, which we extract and purify before running the relevant characterization services; 2) laminarin samples that have undergone initial isolation and purification; 3) finished products containing laminarin, such as supplements, cosmetic formulations, and pharmaceuticals; 4) laminarin experimental samples used in research and development studies.
At CD BioGlyco, our laminarin characterization services are designed to provide critical data to support the research of scientists and industry professionals. Whether for academic research, product development, or quality assurance, our comprehensive analysis will provide you with the insights you need. Please feel free to contact us for detailed protocols on laminarin characterization.
Reference
- Kadam, S.U.; et al. Laminarin from Irish brown seaweeds Ascophyllum nodosum and Laminaria hyperborea: Ultrasound assisted extraction, characterization and bioactivity. Marine drugs. 2015, 13(7): 4270-4280.
