Separation and Purification of Marine Glycan

Separation and Purification of Marine Glycan at CD BioGlyco

Marine glycans have attracted much attention because of their unique structure and biological activity, and their extraction and application have become one of the current research hotspots in the field of marine biotechnology. At CD BioGlyco, our state-of-the-art marine research laboratory keeps up with research trends. We aim to provide our clients with unrivaled Marine Glycomics and Marine Glycoproteomics research services. In particular, our marine glycan isolation and purification services include but are not limited to the following:

• We obtain pure marine polysaccharides by adding organic solvents (e.g., alcohols, ethers, etc.) to the marine polysaccharide solution to cause insoluble precipitation, followed by centrifugation, washing, and drying steps.
• We utilize suitable membranes (e.g. ultrafiltration membranes, reverse osmosis membranes, etc.) to separate the marine glycan solution. By controlling the membrane's retention size and operating parameters (e.g. pressure, flow rate, etc.), impurities and smaller molecular weights are separated, and pure marine polysaccharide is obtained.
• We utilize resins or cellulose matrices with ion exchange functions to adsorb impurity ions on the resin surface, while elution and purification of marine glycans are achieved by adjusting the pH or salt concentration of the solution.
• We pass the marine glycan solution through gel column chromatography. According to the molecular weight and spatial structure of marine glycan, by controlling the solution flow rate and column chromatography conditions, the marine glycan with different molecular weights migrates through the column at different rates to realize pure separation.

In addition to the separation and purification services of marine glycan, we also offer a comprehensive range of services related to marine glycan analysis and research, which include Release, Derivatization, and Characterization services.

Fig.1 Commonly used techniques for the isolation and purification of marine glycans. (CD BioGlyco)

Publication Data

Technology: High-performance liquid chromatography, mass spectrometry, scanning electron microscope, and time-of-flight mass spectrometry

Journal: Chromatography

IF: 1.7

Published: 2015

Results: This article focuses on the development of monolithic column materials for the separation and analysis of glycans, including marine glycans. The article describes several non-traditional methods for preparing boronic acid affinity monolithic columns, including the use of the functional monomer 3-acrylamidophenylboronic acid in in-situ polymerization to prepare monolithic columns, the use of metal organogels as templates for monolithic columns, and the use of molecularly imprinted techniques to prepare monolithic columns. These monolithic column materials were used to enrich and analyze glycoconjugates in biological samples such as glycopeptides and glycoproteins. In addition, the article describes the development of mixed-mode monolithic columns to provide different separation modes, such as ion exchange and hydrophobic modes, for the separation of glycans and glycoproteins.

Fig.2 The challenges in the separation and analysis of glycans. (Alla & Stine, 2015)

Applications

• Marine polysaccharides are used as additives with the functions of thickening, stabilizing emulsification, antioxidant, and antibacterial.
• Marine glycans is applied as carriers or controlled release systems for drugs, enhancing solubility, stability, and bioavailability.
• By varying the surface properties and size of marine glycan nanoparticles, researchers can effectively encapsulate drugs within them for targeted delivery to specific sites in the body.

Highlights

• We retain the bioactivity and physiological functions of marine glycoconjugates through appropriate extraction and purification techniques, which are conducive to further bioactivity research and application development.
• Our extraction and purification technologies have a wide range of application potential for your research in various fields.
• We use state-of-the-art separation and purification methods combined with a variety of analytical techniques to help you quickly and efficiently isolate different types of glycoconjugates from complex marine biological samples.

Frequently Asked Questions

Q1: What are the challenges in the separation and analysis of glycans?

The isolation and analysis of marine glycans face complexity, low extraction efficiency, difficulties in purification and enrichment, complex structural identification, and functional evaluation challenges. These challenges require the integrated use of multiple analytical techniques and methods, as well as the continuous improvement and optimization of existing technical means to effectively obtain high-purity glycan samples and accurately evaluate their structures and functions.

Q2: How to deal with problems during separation and purification?

During the separation and purification process, problems such as low recoveries and the presence of contaminants may be encountered. Our methods to deal with these problems include optimizing the operation steps, selecting suitable separation media, optimizing the elution conditions, etc.

CD BioGlyco is a cutting-edge biotechnology platform that provides innovative marine glycan separation and purification services. We contribute to the development of new marine glycan products and solutions. If you have any specific questions or if there's anything else I can assist you with, feel free to contact us!